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Pathotyping of Newcastle Disease Virus: a Novel Single BsaHI Digestion Method of Detection and Differentiation of Avirulent Strains (Lentogenic and Mesogenic Vaccine Strains) from Virulent Virus

We provide a novel single restriction enzyme (RE; BsaHI) digestion approach for detecting distinct pathotypes of Newcastle disease virus (NDV). After scanning 4,000 F gene nucleotide sequences in the NCBI database, we discovered a single RE (BsaHI) digestion site in the cleavage site. APMV-I “F gene...

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Detalles Bibliográficos
Autores principales: Desingu, Perumal Arumugam, Singh, Shambhu Dayal, Dhama, Kuldeep, Vinodhkumar, Obli Rajendran, Nagarajan, K., Singh, Rajendra, Malik, Yashpal Singh, Singh, Raj Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8653816/
https://www.ncbi.nlm.nih.gov/pubmed/34878298
http://dx.doi.org/10.1128/spectrum.00989-21

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