Cargando…
A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse
Antimicrobial resistance (AMR) is an increasing and urgent issue for human health worldwide, as it leads to the reduction of available antibiotics to treat bacterial infections, in turn increasing hospital stays and lethality. Therefore, the study and genomic surveillance of bacterial carriers of re...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654192/ https://www.ncbi.nlm.nih.gov/pubmed/34899623 http://dx.doi.org/10.3389/fmicb.2021.732324 |
_version_ | 1784611813950750720 |
---|---|
author | Janssen, Luis de Almeida, Felipe Marques Damasceno, Thais Amanda Silva Baptista, Rodrigo de Paula Pappas, Georgios Joannis de Campos, Tatiana Amabile Martins, Vicente de Paulo |
author_facet | Janssen, Luis de Almeida, Felipe Marques Damasceno, Thais Amanda Silva Baptista, Rodrigo de Paula Pappas, Georgios Joannis de Campos, Tatiana Amabile Martins, Vicente de Paulo |
author_sort | Janssen, Luis |
collection | PubMed |
description | Antimicrobial resistance (AMR) is an increasing and urgent issue for human health worldwide, as it leads to the reduction of available antibiotics to treat bacterial infections, in turn increasing hospital stays and lethality. Therefore, the study and genomic surveillance of bacterial carriers of resistance in and outside of clinical settings is of utter importance. A colony of multidrug resistant (MDR) bacteria identified as Klebsiella spp., by 16S rDNA amplicon sequencing, has been isolated from an urban lake in Brazil, during a drug-degrading bacterial prospection. Genomic analyses revealed the bacteria as Klebsiella pneumoniae species. Furthermore, the in silico Multilocus Sequence Typing (MLST) identified the genome as a new sequence type, ST5236. The search for antimicrobial resistance genes (ARGs) detected the presence of genes against beta-lactams, fosfomycin, acriflavine and efflux pumps, as well as genes for heavy metal resistance. Of particular note, an extended-spectrum beta-lactamase gene (blaCTX-M-15) has been detected in close proximity to siphoviridae genes, while a carbapenemase gene (KPC-2) has been found in an extrachromosomal contig, within a novel non-Tn4401 genetic element (NTE(KPC)). An extrachromosomal contig found in the V3 isolate is identical to a contig of a K. pneumoniae isolate from a nearby hospital, which indicates a putative gene flow from the hospital network into Paranoá lake. The discovery of a MDR isolate in this lake is worrisome, as the region has recently undergone periods of water scarcity causing the lake, which receives treated wastewater effluent, and is already used for recreational purposes, to be used as an environmental buffer for drinking water reuse. Altogether, our results indicate an underrepresentation of environmental K. pneumoniae among available genomes, which may hamper the understanding of the population dynamics of the species in the environment and its consequences in the spread of ARGs and virulence genes. |
format | Online Article Text |
id | pubmed-8654192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86541922021-12-09 A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse Janssen, Luis de Almeida, Felipe Marques Damasceno, Thais Amanda Silva Baptista, Rodrigo de Paula Pappas, Georgios Joannis de Campos, Tatiana Amabile Martins, Vicente de Paulo Front Microbiol Microbiology Antimicrobial resistance (AMR) is an increasing and urgent issue for human health worldwide, as it leads to the reduction of available antibiotics to treat bacterial infections, in turn increasing hospital stays and lethality. Therefore, the study and genomic surveillance of bacterial carriers of resistance in and outside of clinical settings is of utter importance. A colony of multidrug resistant (MDR) bacteria identified as Klebsiella spp., by 16S rDNA amplicon sequencing, has been isolated from an urban lake in Brazil, during a drug-degrading bacterial prospection. Genomic analyses revealed the bacteria as Klebsiella pneumoniae species. Furthermore, the in silico Multilocus Sequence Typing (MLST) identified the genome as a new sequence type, ST5236. The search for antimicrobial resistance genes (ARGs) detected the presence of genes against beta-lactams, fosfomycin, acriflavine and efflux pumps, as well as genes for heavy metal resistance. Of particular note, an extended-spectrum beta-lactamase gene (blaCTX-M-15) has been detected in close proximity to siphoviridae genes, while a carbapenemase gene (KPC-2) has been found in an extrachromosomal contig, within a novel non-Tn4401 genetic element (NTE(KPC)). An extrachromosomal contig found in the V3 isolate is identical to a contig of a K. pneumoniae isolate from a nearby hospital, which indicates a putative gene flow from the hospital network into Paranoá lake. The discovery of a MDR isolate in this lake is worrisome, as the region has recently undergone periods of water scarcity causing the lake, which receives treated wastewater effluent, and is already used for recreational purposes, to be used as an environmental buffer for drinking water reuse. Altogether, our results indicate an underrepresentation of environmental K. pneumoniae among available genomes, which may hamper the understanding of the population dynamics of the species in the environment and its consequences in the spread of ARGs and virulence genes. Frontiers Media S.A. 2021-11-24 /pmc/articles/PMC8654192/ /pubmed/34899623 http://dx.doi.org/10.3389/fmicb.2021.732324 Text en Copyright © 2021 Janssen, de Almeida, Damasceno, Baptista, Pappas, de Campos and Martins. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Janssen, Luis de Almeida, Felipe Marques Damasceno, Thais Amanda Silva Baptista, Rodrigo de Paula Pappas, Georgios Joannis de Campos, Tatiana Amabile Martins, Vicente de Paulo A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse |
title | A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse |
title_full | A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse |
title_fullStr | A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse |
title_full_unstemmed | A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse |
title_short | A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse |
title_sort | novel multidrug resistant, non-tn4401 genetic element-bearing, strain of klebsiella pneumoniae isolated from an urban lake with drinking and recreational water reuse |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654192/ https://www.ncbi.nlm.nih.gov/pubmed/34899623 http://dx.doi.org/10.3389/fmicb.2021.732324 |
work_keys_str_mv | AT janssenluis anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT dealmeidafelipemarques anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT damascenothaisamandasilva anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT baptistarodrigodepaula anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT pappasgeorgiosjoannis anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT decampostatianaamabile anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT martinsvicentedepaulo anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT janssenluis novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT dealmeidafelipemarques novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT damascenothaisamandasilva novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT baptistarodrigodepaula novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT pappasgeorgiosjoannis novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT decampostatianaamabile novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT martinsvicentedepaulo novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse |