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Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius

Purpose of the study: Marine fungi of Mauritius have been poorly studied. There are numerous reports on the bioactive secondary metabolites that are produced by fungi around the world. Yet, research on the molecular characterisation and the pharmaceutical potential of marine fungi in Mauritius is ra...

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Autores principales: Wong Chin, Jessica Mélanie, Puchooa, Daneshwar, Bahorun, Theeshan, Jeewon, Rajesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654394/
https://www.ncbi.nlm.nih.gov/pubmed/34900379
http://dx.doi.org/10.1080/21501203.2021.1895347
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author Wong Chin, Jessica Mélanie
Puchooa, Daneshwar
Bahorun, Theeshan
Jeewon, Rajesh
author_facet Wong Chin, Jessica Mélanie
Puchooa, Daneshwar
Bahorun, Theeshan
Jeewon, Rajesh
author_sort Wong Chin, Jessica Mélanie
collection PubMed
description Purpose of the study: Marine fungi of Mauritius have been poorly studied. There are numerous reports on the bioactive secondary metabolites that are produced by fungi around the world. Yet, research on the molecular characterisation and the pharmaceutical potential of marine fungi in Mauritius is rather scanty. Method: The samples, which consisted of three sponges Haliclona sp., Iotrochota sp. and Biemna sp. and two brown algae Turbinaria conoides and Sargassum portierianum, were collected in the North of Mauritius during winter. No sporulating structures were observed from the fungal cultures making morphological analysis impossible. The molecular characterisation of the selected isolates was carried out by the amplification of the ITS regions and phylogenetic analysis. The antimicrobial properties were then determined using the disc diffusion and the minimum inhibitory concentration (MIC) assay. Results: Genus level identification was made from molecular data and for some isolates, species-level identification was even possible. Twelve fungi that showed the best antimicrobial properties were identified as Peniophora sp., Aspergillus cristatus, Acremonium sp., Cordyceps memorabilis, Aspergillus ochraceus, Biscogniauxia sp., Aspergillus keratitidis, Exserohilum rostratum, Chromocleista sp., Nigrospora oryzae, Aspergillus flavipes and Mycosphaerella. The lowest MIC result of 0.0098 mg/mL was obtained with Chromocleista sp. mycelium extract against Staphylococcus aureus. The MIC of the mycelium extracts was lower than the broth extracts for most isolates indicating that the antimicrobial compounds are not secreted. Conclusion: Marine fungi from the Mauritian waters have immense potential in the search for natural products against antibiotic-resistant bacteria.
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spelling pubmed-86543942021-12-09 Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius Wong Chin, Jessica Mélanie Puchooa, Daneshwar Bahorun, Theeshan Jeewon, Rajesh Mycology Research Article Purpose of the study: Marine fungi of Mauritius have been poorly studied. There are numerous reports on the bioactive secondary metabolites that are produced by fungi around the world. Yet, research on the molecular characterisation and the pharmaceutical potential of marine fungi in Mauritius is rather scanty. Method: The samples, which consisted of three sponges Haliclona sp., Iotrochota sp. and Biemna sp. and two brown algae Turbinaria conoides and Sargassum portierianum, were collected in the North of Mauritius during winter. No sporulating structures were observed from the fungal cultures making morphological analysis impossible. The molecular characterisation of the selected isolates was carried out by the amplification of the ITS regions and phylogenetic analysis. The antimicrobial properties were then determined using the disc diffusion and the minimum inhibitory concentration (MIC) assay. Results: Genus level identification was made from molecular data and for some isolates, species-level identification was even possible. Twelve fungi that showed the best antimicrobial properties were identified as Peniophora sp., Aspergillus cristatus, Acremonium sp., Cordyceps memorabilis, Aspergillus ochraceus, Biscogniauxia sp., Aspergillus keratitidis, Exserohilum rostratum, Chromocleista sp., Nigrospora oryzae, Aspergillus flavipes and Mycosphaerella. The lowest MIC result of 0.0098 mg/mL was obtained with Chromocleista sp. mycelium extract against Staphylococcus aureus. The MIC of the mycelium extracts was lower than the broth extracts for most isolates indicating that the antimicrobial compounds are not secreted. Conclusion: Marine fungi from the Mauritian waters have immense potential in the search for natural products against antibiotic-resistant bacteria. Taylor & Francis 2021-04-22 /pmc/articles/PMC8654394/ /pubmed/34900379 http://dx.doi.org/10.1080/21501203.2021.1895347 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wong Chin, Jessica Mélanie
Puchooa, Daneshwar
Bahorun, Theeshan
Jeewon, Rajesh
Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius
title Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius
title_full Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius
title_fullStr Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius
title_full_unstemmed Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius
title_short Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius
title_sort antimicrobial properties of marine fungi from sponges and brown algae of mauritius
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654394/
https://www.ncbi.nlm.nih.gov/pubmed/34900379
http://dx.doi.org/10.1080/21501203.2021.1895347
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