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Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats
BACKGROUND AND AIM: Fibrin forms in the coagulation process, enhancing local hemostatic properties and promoting wound healing. The study aimed to evaluate the efficacy of bubaline-derived fibrin glue in silk ligature-induced periodontitis rats. MATERIALS AND METHODS: Bubaline blood–derived fibrin g...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654744/ https://www.ncbi.nlm.nih.gov/pubmed/34903915 http://dx.doi.org/10.14202/vetworld.2021.2602-2612 |
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author | Banyatworakul, Poranee Pirarat, Nopadon Sirisawadi, Sujin Osathanon, Thanaphum Kalpravidh, Chanin |
author_facet | Banyatworakul, Poranee Pirarat, Nopadon Sirisawadi, Sujin Osathanon, Thanaphum Kalpravidh, Chanin |
author_sort | Banyatworakul, Poranee |
collection | PubMed |
description | BACKGROUND AND AIM: Fibrin forms in the coagulation process, enhancing local hemostatic properties and promoting wound healing. The study aimed to evaluate the efficacy of bubaline-derived fibrin glue in silk ligature-induced periodontitis rats. MATERIALS AND METHODS: Bubaline blood–derived fibrin glue was prepared using cryoprecipitation and cryocentrifugation. Periodontitis was induced in rats by placing 5-0 silk ligatures around the mandibular first molars. The animals were divided into two groups: (1) Non-treatment and (2) bubaline fibrin glue–treated groups. Plaque, gingival inflammation, and mobility index were scored on days 1, 7, and 14 after intervention. Histological examinations were performed. The mRNA expression of inflammatory cytokines and growth factors was evaluated using a real-time polymerase chain reaction. Ligature-induced periodontitis was confirmed by the increase in inflammatory cell infiltration as well as histological bone and attachment loss. RESULTS: Compared to the non-treatment group, bubaline fibrin glue application reduced mononuclear cell infiltration into periodontal tissues corresponding to the reduction of collagen destruction. On days 7 and 14 after intervention, the inflammatory score and histological attachment loss were significantly lower in the bubaline fibrin glue–treated group than in the non-treatment group. A significant reduction in histological bone loss was observed in the treated group on day 7. Bubaline fibrin glue application led to a significant reduction of Tnfa and Il1b mRNA levels, while an increased expression of Pdgfa, Tgfb1, and Il10 was observed compared with the control. CONCLUSION: Bubaline fibrin glue could be beneficial in periodontitis treatment aiming to reduce inflammation and delay the progression of periodontal disease. |
format | Online Article Text |
id | pubmed-8654744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-86547442021-12-12 Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats Banyatworakul, Poranee Pirarat, Nopadon Sirisawadi, Sujin Osathanon, Thanaphum Kalpravidh, Chanin Vet World Research Article BACKGROUND AND AIM: Fibrin forms in the coagulation process, enhancing local hemostatic properties and promoting wound healing. The study aimed to evaluate the efficacy of bubaline-derived fibrin glue in silk ligature-induced periodontitis rats. MATERIALS AND METHODS: Bubaline blood–derived fibrin glue was prepared using cryoprecipitation and cryocentrifugation. Periodontitis was induced in rats by placing 5-0 silk ligatures around the mandibular first molars. The animals were divided into two groups: (1) Non-treatment and (2) bubaline fibrin glue–treated groups. Plaque, gingival inflammation, and mobility index were scored on days 1, 7, and 14 after intervention. Histological examinations were performed. The mRNA expression of inflammatory cytokines and growth factors was evaluated using a real-time polymerase chain reaction. Ligature-induced periodontitis was confirmed by the increase in inflammatory cell infiltration as well as histological bone and attachment loss. RESULTS: Compared to the non-treatment group, bubaline fibrin glue application reduced mononuclear cell infiltration into periodontal tissues corresponding to the reduction of collagen destruction. On days 7 and 14 after intervention, the inflammatory score and histological attachment loss were significantly lower in the bubaline fibrin glue–treated group than in the non-treatment group. A significant reduction in histological bone loss was observed in the treated group on day 7. Bubaline fibrin glue application led to a significant reduction of Tnfa and Il1b mRNA levels, while an increased expression of Pdgfa, Tgfb1, and Il10 was observed compared with the control. CONCLUSION: Bubaline fibrin glue could be beneficial in periodontitis treatment aiming to reduce inflammation and delay the progression of periodontal disease. Veterinary World 2021-10 2021-10-06 /pmc/articles/PMC8654744/ /pubmed/34903915 http://dx.doi.org/10.14202/vetworld.2021.2602-2612 Text en Copyright: © Banyatworakul, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Banyatworakul, Poranee Pirarat, Nopadon Sirisawadi, Sujin Osathanon, Thanaphum Kalpravidh, Chanin Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats |
title | Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats |
title_full | Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats |
title_fullStr | Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats |
title_full_unstemmed | Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats |
title_short | Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats |
title_sort | efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in wistar rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654744/ https://www.ncbi.nlm.nih.gov/pubmed/34903915 http://dx.doi.org/10.14202/vetworld.2021.2602-2612 |
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