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Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh
BACKGROUND AND AIM: Necrotic enteritis (NE) is one of the most prevalent diseases in broiler poultry caused by Clostridium perfringens connected with significant economic losses. A cross-sectional survey was conducted in Mymensingh district of Bangladesh to assess the prevalence of C. perfringens th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654751/ https://www.ncbi.nlm.nih.gov/pubmed/34903943 http://dx.doi.org/10.14202/vetworld.2021.2809-2816 |
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author | Tresha, Arunima Oarin Arif, Mohammad Islam, Sk Shaheenur Haque, A. K. M. Ziaul Rahman, Md. Tanvir Kabir, S. M. Lutful |
author_facet | Tresha, Arunima Oarin Arif, Mohammad Islam, Sk Shaheenur Haque, A. K. M. Ziaul Rahman, Md. Tanvir Kabir, S. M. Lutful |
author_sort | Tresha, Arunima Oarin |
collection | PubMed |
description | BACKGROUND AND AIM: Necrotic enteritis (NE) is one of the most prevalent diseases in broiler poultry caused by Clostridium perfringens connected with significant economic losses. A cross-sectional survey was conducted in Mymensingh district of Bangladesh to assess the prevalence of C. perfringens through toxinotyping molecular assay and confirm the risk factors for NE, including antimicrobial-resistant (AMR) status of the isolates. MATERIALS AND METHODS: We included 40 small-scale commercial broiler farms randomly selected from two subdistricts of Mymensingh district of Bangladesh. As an individual sample, 240 cloacal swabs, and as a pooled sample, 40 drinking water, 40 workers’ hand washing, 40 litter swab, and 40 feed samples were collected and evaluated by culture, biochemical, and molecular assays. A pretested semi-structured interview questionnaire was employed to capture flock-level data on risk factors from the farm owners. The flock-level data on risk factors were assessed through univariable and multivariable logistic regression analyses with p<0.05 was considered statistically significant. RESULTS: Overall flock-level prevalence of C. perfringens was estimated to be 10.3% (95% confidence interval [CI] 7.5-13.6%). Litter swab (pooled) was found to be highly contaminated with C. perfringens (25.0%, 95% CI: 12.7-41.2%) followed by the cloacal swab (10.4%, 95% CI: 6.9-15.0%) and feed sample (5.0%, 95% CI: 0.6-16.9%). History of coccidia infection (Adjusted odds ratio =33.01, 95% CI: 2.14-507.59, p=0.01) was significantly associated with flock-level C. perfringens infection status. In this study, 78.1% isolates were found as multidrug-resistant as they demonstrated resistance to 3-5 antimicrobial agents. CONCLUSION: Evidence-based control options need to be taken through the uses of prebiotics and probiotics, biosecurity, and hygienic measurement, including control of coccidia infection, is needed to lessen the NE infection and AMR related to this pathogen in small-scale commercial broiler poultry. |
format | Online Article Text |
id | pubmed-8654751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-86547512021-12-12 Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh Tresha, Arunima Oarin Arif, Mohammad Islam, Sk Shaheenur Haque, A. K. M. Ziaul Rahman, Md. Tanvir Kabir, S. M. Lutful Vet World Research Article BACKGROUND AND AIM: Necrotic enteritis (NE) is one of the most prevalent diseases in broiler poultry caused by Clostridium perfringens connected with significant economic losses. A cross-sectional survey was conducted in Mymensingh district of Bangladesh to assess the prevalence of C. perfringens through toxinotyping molecular assay and confirm the risk factors for NE, including antimicrobial-resistant (AMR) status of the isolates. MATERIALS AND METHODS: We included 40 small-scale commercial broiler farms randomly selected from two subdistricts of Mymensingh district of Bangladesh. As an individual sample, 240 cloacal swabs, and as a pooled sample, 40 drinking water, 40 workers’ hand washing, 40 litter swab, and 40 feed samples were collected and evaluated by culture, biochemical, and molecular assays. A pretested semi-structured interview questionnaire was employed to capture flock-level data on risk factors from the farm owners. The flock-level data on risk factors were assessed through univariable and multivariable logistic regression analyses with p<0.05 was considered statistically significant. RESULTS: Overall flock-level prevalence of C. perfringens was estimated to be 10.3% (95% confidence interval [CI] 7.5-13.6%). Litter swab (pooled) was found to be highly contaminated with C. perfringens (25.0%, 95% CI: 12.7-41.2%) followed by the cloacal swab (10.4%, 95% CI: 6.9-15.0%) and feed sample (5.0%, 95% CI: 0.6-16.9%). History of coccidia infection (Adjusted odds ratio =33.01, 95% CI: 2.14-507.59, p=0.01) was significantly associated with flock-level C. perfringens infection status. In this study, 78.1% isolates were found as multidrug-resistant as they demonstrated resistance to 3-5 antimicrobial agents. CONCLUSION: Evidence-based control options need to be taken through the uses of prebiotics and probiotics, biosecurity, and hygienic measurement, including control of coccidia infection, is needed to lessen the NE infection and AMR related to this pathogen in small-scale commercial broiler poultry. Veterinary World 2021-10 2021-10-29 /pmc/articles/PMC8654751/ /pubmed/34903943 http://dx.doi.org/10.14202/vetworld.2021.2809-2816 Text en Copyright: © Tresha, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Tresha, Arunima Oarin Arif, Mohammad Islam, Sk Shaheenur Haque, A. K. M. Ziaul Rahman, Md. Tanvir Kabir, S. M. Lutful Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh |
title | Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh |
title_full | Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh |
title_fullStr | Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh |
title_full_unstemmed | Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh |
title_short | Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh |
title_sort | investigation of clostridium perfringens in small-scale commercial broiler flocks in mymensingh district of bangladesh |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654751/ https://www.ncbi.nlm.nih.gov/pubmed/34903943 http://dx.doi.org/10.14202/vetworld.2021.2809-2816 |
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