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First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand

BACKGROUND AND AIM: Anaplasma platys is a blood parasite that infects platelets, causing thrombocytopenia. Rhipicephalus sanguineus ticks are believed to transmit A. platys. To identify A. platys, nested polymerase chain reaction (PCR) has proven to be an effective diagnostic tool. In this study, th...

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Autores principales: Sarker, Biethee Rani, Mitpasa, Thongphet, Macotpet, Arayaporn, Bupata, Pattara-Anong, Sangmaneedet, Somboon, Taweenan, Weerapol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654768/
https://www.ncbi.nlm.nih.gov/pubmed/34903916
http://dx.doi.org/10.14202/vetworld.2021.2613-2619
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author Sarker, Biethee Rani
Mitpasa, Thongphet
Macotpet, Arayaporn
Bupata, Pattara-Anong
Sangmaneedet, Somboon
Taweenan, Weerapol
author_facet Sarker, Biethee Rani
Mitpasa, Thongphet
Macotpet, Arayaporn
Bupata, Pattara-Anong
Sangmaneedet, Somboon
Taweenan, Weerapol
author_sort Sarker, Biethee Rani
collection PubMed
description BACKGROUND AND AIM: Anaplasma platys is a blood parasite that infects platelets, causing thrombocytopenia. Rhipicephalus sanguineus ticks are believed to transmit A. platys. To identify A. platys, nested polymerase chain reaction (PCR) has proven to be an effective diagnostic tool. In this study, the molecular prevalence of A. platys infection in dogs was investigated for the 1(st) time in the Khon Kaen region of Thailand. The association between risk factors and A. platys infection was also evaluated. MATERIALS AND METHODS: A total of 130 blood samples were collected from dogs in Khon Kaen, Thailand. DNA from the samples was extracted and nested PCR was applied for molecular analysis. Platelet count and packed cell volume (PCV) levels were measured. Platelet counts were categorized into four grades: Non-thrombocytopenia (platelets >200,000 cells/μL), mild thrombocytopenia (platelets 150,000-200,000 cells/μL), moderate thrombocytopenia (platelets 100,000-150,000 cells/μL), and severe thrombocytopenia (platelets <100,000 cells/μL). Four categories for PCV levels of >37%, 30-37%, 20-29%, and <20% were defined as no anemia, mild anemia, moderate anemia, and severe anemia, respectively. DNA sequencing was analyzed using BTSeq™ (Barcode-Tagged Sequencing; CELEMICS, Seoul, South Korea) for similarity index. RESULTS: Among the 130 samples, 9 (6.9%) were positive for A. platys infection. There was an association between low platelet count and infection (p<0.05). PCV level was also associated with A. platys infection (p<0.05). DNA sequencing results of the nine positive samples showed similarity to known sequences of A. platys with 99.36-100% nucleotide identity. These results suggested low genetic diversity in A. platys infecting dogs in the Khon Kaen area. CONCLUSION: By amplifying 16S rRNA, A. platys infection was detected in the blood of Thai dogs. Further work should be performed to identify risk factors potentially associated with A. platys infection in dogs in Khon Kaen. Other related factors should also be considered, such as location and breeding, as well as the environmental characteristics of each locality. In addition, sampling a larger number of animals may reveal predictors for the positivity of A. platys in dogs in this region.
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spelling pubmed-86547682021-12-12 First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand Sarker, Biethee Rani Mitpasa, Thongphet Macotpet, Arayaporn Bupata, Pattara-Anong Sangmaneedet, Somboon Taweenan, Weerapol Vet World Research Article BACKGROUND AND AIM: Anaplasma platys is a blood parasite that infects platelets, causing thrombocytopenia. Rhipicephalus sanguineus ticks are believed to transmit A. platys. To identify A. platys, nested polymerase chain reaction (PCR) has proven to be an effective diagnostic tool. In this study, the molecular prevalence of A. platys infection in dogs was investigated for the 1(st) time in the Khon Kaen region of Thailand. The association between risk factors and A. platys infection was also evaluated. MATERIALS AND METHODS: A total of 130 blood samples were collected from dogs in Khon Kaen, Thailand. DNA from the samples was extracted and nested PCR was applied for molecular analysis. Platelet count and packed cell volume (PCV) levels were measured. Platelet counts were categorized into four grades: Non-thrombocytopenia (platelets >200,000 cells/μL), mild thrombocytopenia (platelets 150,000-200,000 cells/μL), moderate thrombocytopenia (platelets 100,000-150,000 cells/μL), and severe thrombocytopenia (platelets <100,000 cells/μL). Four categories for PCV levels of >37%, 30-37%, 20-29%, and <20% were defined as no anemia, mild anemia, moderate anemia, and severe anemia, respectively. DNA sequencing was analyzed using BTSeq™ (Barcode-Tagged Sequencing; CELEMICS, Seoul, South Korea) for similarity index. RESULTS: Among the 130 samples, 9 (6.9%) were positive for A. platys infection. There was an association between low platelet count and infection (p<0.05). PCV level was also associated with A. platys infection (p<0.05). DNA sequencing results of the nine positive samples showed similarity to known sequences of A. platys with 99.36-100% nucleotide identity. These results suggested low genetic diversity in A. platys infecting dogs in the Khon Kaen area. CONCLUSION: By amplifying 16S rRNA, A. platys infection was detected in the blood of Thai dogs. Further work should be performed to identify risk factors potentially associated with A. platys infection in dogs in Khon Kaen. Other related factors should also be considered, such as location and breeding, as well as the environmental characteristics of each locality. In addition, sampling a larger number of animals may reveal predictors for the positivity of A. platys in dogs in this region. Veterinary World 2021-10 2021-10-06 /pmc/articles/PMC8654768/ /pubmed/34903916 http://dx.doi.org/10.14202/vetworld.2021.2613-2619 Text en Copyright: © Sarker, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Sarker, Biethee Rani
Mitpasa, Thongphet
Macotpet, Arayaporn
Bupata, Pattara-Anong
Sangmaneedet, Somboon
Taweenan, Weerapol
First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand
title First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand
title_full First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand
title_fullStr First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand
title_full_unstemmed First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand
title_short First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand
title_sort first report on molecular prevalence and identification of anaplasma platys in dogs in khon kaen, thailand
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654768/
https://www.ncbi.nlm.nih.gov/pubmed/34903916
http://dx.doi.org/10.14202/vetworld.2021.2613-2619
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