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Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model

Babesiosis is one of the most common infections in free-living animals and is rapidly becoming significant among human zoonoses. Cases of acute renal failure in humans caused by Babesia spp. have been described in the literature. The kidneys are characterised by intense blood flow through the blood...

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Autores principales: Albertyńska, Marta, Okła, Hubert, Jasik, Krzysztof, Urbańska-Jasik, Danuta, Pol, Przemysław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654915/
https://www.ncbi.nlm.nih.gov/pubmed/34880327
http://dx.doi.org/10.1038/s41598-021-03079-0
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author Albertyńska, Marta
Okła, Hubert
Jasik, Krzysztof
Urbańska-Jasik, Danuta
Pol, Przemysław
author_facet Albertyńska, Marta
Okła, Hubert
Jasik, Krzysztof
Urbańska-Jasik, Danuta
Pol, Przemysław
author_sort Albertyńska, Marta
collection PubMed
description Babesiosis is one of the most common infections in free-living animals and is rapidly becoming significant among human zoonoses. Cases of acute renal failure in humans caused by Babesia spp. have been described in the literature. The kidneys are characterised by intense blood flow through the blood vessels, which increases the likelihood of contact with the intra-erythrocyte parasite. The aim of this study was to observe the influence of B. microti (ATCC 30221) on renal epithelial cells in vitro cultured (NRK-52E line) and Wistar rats’ kidney. Both NRK-52E cells and rats’ kidney sections were analysed by light microscopy, transmission electron microscopy (TEM) and fluorescence in situ hybridization (FISH). Necrotic changes in renal epithelial cells have been observed in vitro and in vivo. In many cross-sections through the rats’ kidney, adhesion of blood cells to the vascular endothelium, accumulation of erythrocytes and emboli were demonstrated. In NRK-52E culture, elements with a distinctly doubled cell membrane resembling B. microti were found inside the cytoplasm and adjacent to the cell layer. The study indicates a chemotactic tendency for B. microti to adhere to the renal tubules' epithelium, a possibility of piroplasms entering the renal epithelial cells, their proliferation within the cytoplasm and emboli formation.
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spelling pubmed-86549152021-12-09 Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model Albertyńska, Marta Okła, Hubert Jasik, Krzysztof Urbańska-Jasik, Danuta Pol, Przemysław Sci Rep Article Babesiosis is one of the most common infections in free-living animals and is rapidly becoming significant among human zoonoses. Cases of acute renal failure in humans caused by Babesia spp. have been described in the literature. The kidneys are characterised by intense blood flow through the blood vessels, which increases the likelihood of contact with the intra-erythrocyte parasite. The aim of this study was to observe the influence of B. microti (ATCC 30221) on renal epithelial cells in vitro cultured (NRK-52E line) and Wistar rats’ kidney. Both NRK-52E cells and rats’ kidney sections were analysed by light microscopy, transmission electron microscopy (TEM) and fluorescence in situ hybridization (FISH). Necrotic changes in renal epithelial cells have been observed in vitro and in vivo. In many cross-sections through the rats’ kidney, adhesion of blood cells to the vascular endothelium, accumulation of erythrocytes and emboli were demonstrated. In NRK-52E culture, elements with a distinctly doubled cell membrane resembling B. microti were found inside the cytoplasm and adjacent to the cell layer. The study indicates a chemotactic tendency for B. microti to adhere to the renal tubules' epithelium, a possibility of piroplasms entering the renal epithelial cells, their proliferation within the cytoplasm and emboli formation. Nature Publishing Group UK 2021-12-08 /pmc/articles/PMC8654915/ /pubmed/34880327 http://dx.doi.org/10.1038/s41598-021-03079-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Albertyńska, Marta
Okła, Hubert
Jasik, Krzysztof
Urbańska-Jasik, Danuta
Pol, Przemysław
Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model
title Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model
title_full Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model
title_fullStr Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model
title_full_unstemmed Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model
title_short Interactions between Babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model
title_sort interactions between babesia microti merozoites and rat kidney cells in a short-term in vitro culture and animal model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654915/
https://www.ncbi.nlm.nih.gov/pubmed/34880327
http://dx.doi.org/10.1038/s41598-021-03079-0
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