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Single-cell analysis identifies a key role for Hhip in murine coronal suture development
Craniofacial development depends on formation and maintenance of sutures between bones of the skull. In sutures, growth occurs at osteogenic fronts along the edge of each bone, and suture mesenchyme separates adjacent bones. Here, we perform single-cell RNA-seq analysis of the embryonic, wild type m...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655033/ https://www.ncbi.nlm.nih.gov/pubmed/34880220 http://dx.doi.org/10.1038/s41467-021-27402-5 |
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author | Holmes, Greg Gonzalez-Reiche, Ana S. Saturne, Madrikha Motch Perrine, Susan M. Zhou, Xianxiao Borges, Ana C. Shewale, Bhavana Richtsmeier, Joan T. Zhang, Bin van Bakel, Harm Jabs, Ethylin Wang |
author_facet | Holmes, Greg Gonzalez-Reiche, Ana S. Saturne, Madrikha Motch Perrine, Susan M. Zhou, Xianxiao Borges, Ana C. Shewale, Bhavana Richtsmeier, Joan T. Zhang, Bin van Bakel, Harm Jabs, Ethylin Wang |
author_sort | Holmes, Greg |
collection | PubMed |
description | Craniofacial development depends on formation and maintenance of sutures between bones of the skull. In sutures, growth occurs at osteogenic fronts along the edge of each bone, and suture mesenchyme separates adjacent bones. Here, we perform single-cell RNA-seq analysis of the embryonic, wild type murine coronal suture to define its population structure. Seven populations at E16.5 and nine at E18.5 comprise the suture mesenchyme, osteogenic cells, and associated populations. Expression of Hhip, an inhibitor of hedgehog signaling, marks a mesenchymal population distinct from those of other neurocranial sutures. Tracing of the neonatal Hhip-expressing population shows that descendant cells persist in the coronal suture and contribute to calvarial bone growth. In Hhip(−/−) coronal sutures at E18.5, the osteogenic fronts are closely apposed and the suture mesenchyme is depleted with increased hedgehog signaling compared to those of the wild type. Collectively, these data demonstrate that Hhip is required for normal coronal suture development. |
format | Online Article Text |
id | pubmed-8655033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86550332021-12-27 Single-cell analysis identifies a key role for Hhip in murine coronal suture development Holmes, Greg Gonzalez-Reiche, Ana S. Saturne, Madrikha Motch Perrine, Susan M. Zhou, Xianxiao Borges, Ana C. Shewale, Bhavana Richtsmeier, Joan T. Zhang, Bin van Bakel, Harm Jabs, Ethylin Wang Nat Commun Article Craniofacial development depends on formation and maintenance of sutures between bones of the skull. In sutures, growth occurs at osteogenic fronts along the edge of each bone, and suture mesenchyme separates adjacent bones. Here, we perform single-cell RNA-seq analysis of the embryonic, wild type murine coronal suture to define its population structure. Seven populations at E16.5 and nine at E18.5 comprise the suture mesenchyme, osteogenic cells, and associated populations. Expression of Hhip, an inhibitor of hedgehog signaling, marks a mesenchymal population distinct from those of other neurocranial sutures. Tracing of the neonatal Hhip-expressing population shows that descendant cells persist in the coronal suture and contribute to calvarial bone growth. In Hhip(−/−) coronal sutures at E18.5, the osteogenic fronts are closely apposed and the suture mesenchyme is depleted with increased hedgehog signaling compared to those of the wild type. Collectively, these data demonstrate that Hhip is required for normal coronal suture development. Nature Publishing Group UK 2021-12-08 /pmc/articles/PMC8655033/ /pubmed/34880220 http://dx.doi.org/10.1038/s41467-021-27402-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Holmes, Greg Gonzalez-Reiche, Ana S. Saturne, Madrikha Motch Perrine, Susan M. Zhou, Xianxiao Borges, Ana C. Shewale, Bhavana Richtsmeier, Joan T. Zhang, Bin van Bakel, Harm Jabs, Ethylin Wang Single-cell analysis identifies a key role for Hhip in murine coronal suture development |
title | Single-cell analysis identifies a key role for Hhip in murine coronal suture development |
title_full | Single-cell analysis identifies a key role for Hhip in murine coronal suture development |
title_fullStr | Single-cell analysis identifies a key role for Hhip in murine coronal suture development |
title_full_unstemmed | Single-cell analysis identifies a key role for Hhip in murine coronal suture development |
title_short | Single-cell analysis identifies a key role for Hhip in murine coronal suture development |
title_sort | single-cell analysis identifies a key role for hhip in murine coronal suture development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655033/ https://www.ncbi.nlm.nih.gov/pubmed/34880220 http://dx.doi.org/10.1038/s41467-021-27402-5 |
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