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Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
Acute cold induces beige adipocyte protein marker expression in human subcutaneous white adipose tissue (SC WAT) from both the cold treated and contralateral leg, and the immune system regulates SC WAT beiging in mice. Cold treatment significantly increased the gene expression of the macrophage mark...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655049/ https://www.ncbi.nlm.nih.gov/pubmed/34880313 http://dx.doi.org/10.1038/s41598-021-03014-3 |
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author | Finlin, Brian S. Memetimin, Hasiyet Confides, Amy L. Zhu, Beibei Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. |
author_facet | Finlin, Brian S. Memetimin, Hasiyet Confides, Amy L. Zhu, Beibei Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. |
author_sort | Finlin, Brian S. |
collection | PubMed |
description | Acute cold induces beige adipocyte protein marker expression in human subcutaneous white adipose tissue (SC WAT) from both the cold treated and contralateral leg, and the immune system regulates SC WAT beiging in mice. Cold treatment significantly increased the gene expression of the macrophage markers CD68 and 86 in SC WAT. Therefore, we comprehensively investigated the involvement of macrophages in SC WAT beiging in lean and obese humans by immunohistochemistry. Cold treatment significantly increased CD163/CD68 macrophages in SC WAT from the cold treated and contralateral legs of lean and obese subjects, and had similar effects on CD206/CD68 macrophages, whereas the effects on CD86/CD68 macrophages were inconsistent between lean and obese. However, linear regression analysis did not find significant relationships between the change in macrophage numbers and the change in UCP1 protein abundance. A high percentage of CD163 macrophages in SC WAT expressed UCP1, and these UCP1 expressing CD163 macrophages were significantly increased by cold treatment in SC WAT of lean subjects. In conclusion, our results suggest that CD163 macrophages are involved in some aspect of the tissue remodeling that occurs during SC WAT beiging in humans after cold treatment, but they are likely not direct mediators of the beiging process. |
format | Online Article Text |
id | pubmed-8655049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86550492021-12-09 Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans Finlin, Brian S. Memetimin, Hasiyet Confides, Amy L. Zhu, Beibei Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. Sci Rep Article Acute cold induces beige adipocyte protein marker expression in human subcutaneous white adipose tissue (SC WAT) from both the cold treated and contralateral leg, and the immune system regulates SC WAT beiging in mice. Cold treatment significantly increased the gene expression of the macrophage markers CD68 and 86 in SC WAT. Therefore, we comprehensively investigated the involvement of macrophages in SC WAT beiging in lean and obese humans by immunohistochemistry. Cold treatment significantly increased CD163/CD68 macrophages in SC WAT from the cold treated and contralateral legs of lean and obese subjects, and had similar effects on CD206/CD68 macrophages, whereas the effects on CD86/CD68 macrophages were inconsistent between lean and obese. However, linear regression analysis did not find significant relationships between the change in macrophage numbers and the change in UCP1 protein abundance. A high percentage of CD163 macrophages in SC WAT expressed UCP1, and these UCP1 expressing CD163 macrophages were significantly increased by cold treatment in SC WAT of lean subjects. In conclusion, our results suggest that CD163 macrophages are involved in some aspect of the tissue remodeling that occurs during SC WAT beiging in humans after cold treatment, but they are likely not direct mediators of the beiging process. Nature Publishing Group UK 2021-12-08 /pmc/articles/PMC8655049/ /pubmed/34880313 http://dx.doi.org/10.1038/s41598-021-03014-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Finlin, Brian S. Memetimin, Hasiyet Confides, Amy L. Zhu, Beibei Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans |
title | Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans |
title_full | Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans |
title_fullStr | Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans |
title_full_unstemmed | Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans |
title_short | Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans |
title_sort | macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655049/ https://www.ncbi.nlm.nih.gov/pubmed/34880313 http://dx.doi.org/10.1038/s41598-021-03014-3 |
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