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Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans

Acute cold induces beige adipocyte protein marker expression in human subcutaneous white adipose tissue (SC WAT) from both the cold treated and contralateral leg, and the immune system regulates SC WAT beiging in mice. Cold treatment significantly increased the gene expression of the macrophage mark...

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Autores principales: Finlin, Brian S., Memetimin, Hasiyet, Confides, Amy L., Zhu, Beibei, Westgate, Philip M., Dupont-Versteegden, Esther E., Kern, Philip A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655049/
https://www.ncbi.nlm.nih.gov/pubmed/34880313
http://dx.doi.org/10.1038/s41598-021-03014-3
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author Finlin, Brian S.
Memetimin, Hasiyet
Confides, Amy L.
Zhu, Beibei
Westgate, Philip M.
Dupont-Versteegden, Esther E.
Kern, Philip A.
author_facet Finlin, Brian S.
Memetimin, Hasiyet
Confides, Amy L.
Zhu, Beibei
Westgate, Philip M.
Dupont-Versteegden, Esther E.
Kern, Philip A.
author_sort Finlin, Brian S.
collection PubMed
description Acute cold induces beige adipocyte protein marker expression in human subcutaneous white adipose tissue (SC WAT) from both the cold treated and contralateral leg, and the immune system regulates SC WAT beiging in mice. Cold treatment significantly increased the gene expression of the macrophage markers CD68 and 86 in SC WAT. Therefore, we comprehensively investigated the involvement of macrophages in SC WAT beiging in lean and obese humans by immunohistochemistry. Cold treatment significantly increased CD163/CD68 macrophages in SC WAT from the cold treated and contralateral legs of lean and obese subjects, and had similar effects on CD206/CD68 macrophages, whereas the effects on CD86/CD68 macrophages were inconsistent between lean and obese. However, linear regression analysis did not find significant relationships between the change in macrophage numbers and the change in UCP1 protein abundance. A high percentage of CD163 macrophages in SC WAT expressed UCP1, and these UCP1 expressing CD163 macrophages were significantly increased by cold treatment in SC WAT of lean subjects. In conclusion, our results suggest that CD163 macrophages are involved in some aspect of the tissue remodeling that occurs during SC WAT beiging in humans after cold treatment, but they are likely not direct mediators of the beiging process.
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spelling pubmed-86550492021-12-09 Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans Finlin, Brian S. Memetimin, Hasiyet Confides, Amy L. Zhu, Beibei Westgate, Philip M. Dupont-Versteegden, Esther E. Kern, Philip A. Sci Rep Article Acute cold induces beige adipocyte protein marker expression in human subcutaneous white adipose tissue (SC WAT) from both the cold treated and contralateral leg, and the immune system regulates SC WAT beiging in mice. Cold treatment significantly increased the gene expression of the macrophage markers CD68 and 86 in SC WAT. Therefore, we comprehensively investigated the involvement of macrophages in SC WAT beiging in lean and obese humans by immunohistochemistry. Cold treatment significantly increased CD163/CD68 macrophages in SC WAT from the cold treated and contralateral legs of lean and obese subjects, and had similar effects on CD206/CD68 macrophages, whereas the effects on CD86/CD68 macrophages were inconsistent between lean and obese. However, linear regression analysis did not find significant relationships between the change in macrophage numbers and the change in UCP1 protein abundance. A high percentage of CD163 macrophages in SC WAT expressed UCP1, and these UCP1 expressing CD163 macrophages were significantly increased by cold treatment in SC WAT of lean subjects. In conclusion, our results suggest that CD163 macrophages are involved in some aspect of the tissue remodeling that occurs during SC WAT beiging in humans after cold treatment, but they are likely not direct mediators of the beiging process. Nature Publishing Group UK 2021-12-08 /pmc/articles/PMC8655049/ /pubmed/34880313 http://dx.doi.org/10.1038/s41598-021-03014-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Finlin, Brian S.
Memetimin, Hasiyet
Confides, Amy L.
Zhu, Beibei
Westgate, Philip M.
Dupont-Versteegden, Esther E.
Kern, Philip A.
Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
title Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
title_full Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
title_fullStr Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
title_full_unstemmed Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
title_short Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
title_sort macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655049/
https://www.ncbi.nlm.nih.gov/pubmed/34880313
http://dx.doi.org/10.1038/s41598-021-03014-3
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