Robust, fiducial-free drift correction for super-resolution imaging

We describe a robust, fiducial-free method of drift correction for use in single molecule localization-based super-resolution methods. The method combines periodic 3D registration of the sample using brightfield images with a fast post-processing algorithm that corrects residual registration errors...

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Detalles Bibliográficos
Autores principales: Wester, Michael J., Schodt, David J., Mazloom-Farsibaf, Hanieh, Fazel, Mohamadreza, Pallikkuth, Sandeep, Lidke, Keith A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655078/
https://www.ncbi.nlm.nih.gov/pubmed/34880301
http://dx.doi.org/10.1038/s41598-021-02850-7
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author Wester, Michael J.
Schodt, David J.
Mazloom-Farsibaf, Hanieh
Fazel, Mohamadreza
Pallikkuth, Sandeep
Lidke, Keith A.
author_facet Wester, Michael J.
Schodt, David J.
Mazloom-Farsibaf, Hanieh
Fazel, Mohamadreza
Pallikkuth, Sandeep
Lidke, Keith A.
author_sort Wester, Michael J.
collection PubMed
description We describe a robust, fiducial-free method of drift correction for use in single molecule localization-based super-resolution methods. The method combines periodic 3D registration of the sample using brightfield images with a fast post-processing algorithm that corrects residual registration errors and drift between registration events. The method is robust to low numbers of collected localizations, requires no specialized hardware, and provides stability and drift correction for an indefinite time period.
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spelling pubmed-86550782021-12-13 Robust, fiducial-free drift correction for super-resolution imaging Wester, Michael J. Schodt, David J. Mazloom-Farsibaf, Hanieh Fazel, Mohamadreza Pallikkuth, Sandeep Lidke, Keith A. Sci Rep Article We describe a robust, fiducial-free method of drift correction for use in single molecule localization-based super-resolution methods. The method combines periodic 3D registration of the sample using brightfield images with a fast post-processing algorithm that corrects residual registration errors and drift between registration events. The method is robust to low numbers of collected localizations, requires no specialized hardware, and provides stability and drift correction for an indefinite time period. Nature Publishing Group UK 2021-12-08 /pmc/articles/PMC8655078/ /pubmed/34880301 http://dx.doi.org/10.1038/s41598-021-02850-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wester, Michael J.
Schodt, David J.
Mazloom-Farsibaf, Hanieh
Fazel, Mohamadreza
Pallikkuth, Sandeep
Lidke, Keith A.
Robust, fiducial-free drift correction for super-resolution imaging
title Robust, fiducial-free drift correction for super-resolution imaging
title_full Robust, fiducial-free drift correction for super-resolution imaging
title_fullStr Robust, fiducial-free drift correction for super-resolution imaging
title_full_unstemmed Robust, fiducial-free drift correction for super-resolution imaging
title_short Robust, fiducial-free drift correction for super-resolution imaging
title_sort robust, fiducial-free drift correction for super-resolution imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655078/
https://www.ncbi.nlm.nih.gov/pubmed/34880301
http://dx.doi.org/10.1038/s41598-021-02850-7
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