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Dimensional study of prostate cancer using stereological tools

This study analyzes the dimensional changes of the glands from prostate cancer by applying stereology to estimate the variations in volume, length, surface, and cellular densities of tumor acini. Normal and tumor acini were visualized using immunohistochemistry for cytokeratin18. On immunostained se...

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Detalles Bibliográficos
Autores principales: Santamaría, Luis, Ingelmo, Ildefonso, Teba, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655212/
https://www.ncbi.nlm.nih.gov/pubmed/34355401
http://dx.doi.org/10.1111/joa.13524
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author Santamaría, Luis
Ingelmo, Ildefonso
Teba, Fernando
author_facet Santamaría, Luis
Ingelmo, Ildefonso
Teba, Fernando
author_sort Santamaría, Luis
collection PubMed
description This study analyzes the dimensional changes of the glands from prostate cancer by applying stereology to estimate the variations in volume, length, surface, and cellular densities of tumor acini. Normal and tumor acini were visualized using immunohistochemistry for cytokeratin18. On immunostained sections, parameters related to the dimensions and cell population of prostate acini were measured. The immunohistochemical expression of proliferative cell nuclear antigen was also measured to correlate the quantitative changes estimated with the proliferative activity of the epithelium. The average cell volume in normal and tumor epithelium was estimated using the method of the nucleator. The relative size of the acini was similar in the carcinoma compared with the normal prostate. Within the acini, the fraction of acinar volume occupied by the epithelium was significantly higher in cancer than in the nontumor prostate. Conversely, the glandular lumen of the cancer acini is lower than in the normal acini. The significant increase of acinar length density in the carcinoma indicates that the glandular tree's growth in the carcinoma is higher and with more branches than in the case of nonneoplastic glands. The basal surface density is higher in the carcinoma than in the controls. The number of epithelial cells per unit length of acini was significantly decreased in the neoplastic glands. This "dilution" of the cell population along the cancer acinus can be explained by the significant increase in the tumor cell's mean cell volume.
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spelling pubmed-86552122021-12-20 Dimensional study of prostate cancer using stereological tools Santamaría, Luis Ingelmo, Ildefonso Teba, Fernando J Anat Original Papers This study analyzes the dimensional changes of the glands from prostate cancer by applying stereology to estimate the variations in volume, length, surface, and cellular densities of tumor acini. Normal and tumor acini were visualized using immunohistochemistry for cytokeratin18. On immunostained sections, parameters related to the dimensions and cell population of prostate acini were measured. The immunohistochemical expression of proliferative cell nuclear antigen was also measured to correlate the quantitative changes estimated with the proliferative activity of the epithelium. The average cell volume in normal and tumor epithelium was estimated using the method of the nucleator. The relative size of the acini was similar in the carcinoma compared with the normal prostate. Within the acini, the fraction of acinar volume occupied by the epithelium was significantly higher in cancer than in the nontumor prostate. Conversely, the glandular lumen of the cancer acini is lower than in the normal acini. The significant increase of acinar length density in the carcinoma indicates that the glandular tree's growth in the carcinoma is higher and with more branches than in the case of nonneoplastic glands. The basal surface density is higher in the carcinoma than in the controls. The number of epithelial cells per unit length of acini was significantly decreased in the neoplastic glands. This "dilution" of the cell population along the cancer acinus can be explained by the significant increase in the tumor cell's mean cell volume. John Wiley and Sons Inc. 2021-08-05 2022-01 /pmc/articles/PMC8655212/ /pubmed/34355401 http://dx.doi.org/10.1111/joa.13524 Text en © 2021 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Papers
Santamaría, Luis
Ingelmo, Ildefonso
Teba, Fernando
Dimensional study of prostate cancer using stereological tools
title Dimensional study of prostate cancer using stereological tools
title_full Dimensional study of prostate cancer using stereological tools
title_fullStr Dimensional study of prostate cancer using stereological tools
title_full_unstemmed Dimensional study of prostate cancer using stereological tools
title_short Dimensional study of prostate cancer using stereological tools
title_sort dimensional study of prostate cancer using stereological tools
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655212/
https://www.ncbi.nlm.nih.gov/pubmed/34355401
http://dx.doi.org/10.1111/joa.13524
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