Cargando…

AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia

Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Shimin, Yao, Longfeng, Zhang, Wenjuan, Cheng, Zihang, Hu, Can, Liu, Hang, Yan, Yanling, Shi, Anbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655793/
https://www.ncbi.nlm.nih.gov/pubmed/34901019
http://dx.doi.org/10.3389/fcell.2021.774401
_version_ 1784612146334662656
author Wang, Shimin
Yao, Longfeng
Zhang, Wenjuan
Cheng, Zihang
Hu, Can
Liu, Hang
Yan, Yanling
Shi, Anbing
author_facet Wang, Shimin
Yao, Longfeng
Zhang, Wenjuan
Cheng, Zihang
Hu, Can
Liu, Hang
Yan, Yanling
Shi, Anbing
author_sort Wang, Shimin
collection PubMed
description Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF-1 appeared abnormally on the apical membrane. SMAP-1 colocalized with AP-1 on the TGN. The integrity of AP-1 is required for the subcellular presence of SMAP-1. Moreover, we found that the loss of SMAP-1 reduced clathrin-positive structures in the cytosol, suggesting that SMAP-1 has a regulatory role in clathrin assembly on the TGN. Functional experiments showed that overexpressing clathrin effectively alleviated exocytic defects due to the lack of SMAP-1, corroborating the role of SMAP-1 in promoting the assembly of clathrin on the TGN. Together, our results suggested that the AP-1 complex regulates the TGN localization of SMAP-1, promoting clathrin assembly to ensure polarized conventional secretion in C. elegans intestinal epithelia.
format Online
Article
Text
id pubmed-8655793
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86557932021-12-10 AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia Wang, Shimin Yao, Longfeng Zhang, Wenjuan Cheng, Zihang Hu, Can Liu, Hang Yan, Yanling Shi, Anbing Front Cell Dev Biol Cell and Developmental Biology Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF-1 appeared abnormally on the apical membrane. SMAP-1 colocalized with AP-1 on the TGN. The integrity of AP-1 is required for the subcellular presence of SMAP-1. Moreover, we found that the loss of SMAP-1 reduced clathrin-positive structures in the cytosol, suggesting that SMAP-1 has a regulatory role in clathrin assembly on the TGN. Functional experiments showed that overexpressing clathrin effectively alleviated exocytic defects due to the lack of SMAP-1, corroborating the role of SMAP-1 in promoting the assembly of clathrin on the TGN. Together, our results suggested that the AP-1 complex regulates the TGN localization of SMAP-1, promoting clathrin assembly to ensure polarized conventional secretion in C. elegans intestinal epithelia. Frontiers Media S.A. 2021-11-25 /pmc/articles/PMC8655793/ /pubmed/34901019 http://dx.doi.org/10.3389/fcell.2021.774401 Text en Copyright © 2021 Wang, Yao, Zhang, Cheng, Hu, Liu, Yan and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Wang, Shimin
Yao, Longfeng
Zhang, Wenjuan
Cheng, Zihang
Hu, Can
Liu, Hang
Yan, Yanling
Shi, Anbing
AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_full AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_fullStr AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_full_unstemmed AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_short AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_sort ap-1 recruits smap-1/smaps to the trans-golgi network to promote sorting in polarized epithelia
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655793/
https://www.ncbi.nlm.nih.gov/pubmed/34901019
http://dx.doi.org/10.3389/fcell.2021.774401
work_keys_str_mv AT wangshimin ap1recruitssmap1smapstothetransgolginetworktopromotesortinginpolarizedepithelia
AT yaolongfeng ap1recruitssmap1smapstothetransgolginetworktopromotesortinginpolarizedepithelia
AT zhangwenjuan ap1recruitssmap1smapstothetransgolginetworktopromotesortinginpolarizedepithelia
AT chengzihang ap1recruitssmap1smapstothetransgolginetworktopromotesortinginpolarizedepithelia
AT hucan ap1recruitssmap1smapstothetransgolginetworktopromotesortinginpolarizedepithelia
AT liuhang ap1recruitssmap1smapstothetransgolginetworktopromotesortinginpolarizedepithelia
AT yanyanling ap1recruitssmap1smapstothetransgolginetworktopromotesortinginpolarizedepithelia
AT shianbing ap1recruitssmap1smapstothetransgolginetworktopromotesortinginpolarizedepithelia