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Ionic-Liquid-Stabilized TiO(2) Nanostructures: A Platform for Detection of Hydrogen Peroxide
[Image: see text] Hydrogen peroxide (H(2)O(2)) acts as a signaling molecule to direct different biological processes. However, its excess amount results in oxidative stress, which causes the onset of different types of cancers. TiO(2) nanostructure was synthesized by a facile hydrothermal method. Th...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655897/ https://www.ncbi.nlm.nih.gov/pubmed/34901624 http://dx.doi.org/10.1021/acsomega.1c04548 |
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author | Nishan, Umar Haq, Shams Ul Rahim, Abdur Asad, Muhammad Badshah, Amir Ali Shah, Azhar-ul-Haq Iqbal, Anwar Muhammad, Nawshad |
author_facet | Nishan, Umar Haq, Shams Ul Rahim, Abdur Asad, Muhammad Badshah, Amir Ali Shah, Azhar-ul-Haq Iqbal, Anwar Muhammad, Nawshad |
author_sort | Nishan, Umar |
collection | PubMed |
description | [Image: see text] Hydrogen peroxide (H(2)O(2)) acts as a signaling molecule to direct different biological processes. However, its excess amount results in oxidative stress, which causes the onset of different types of cancers. TiO(2) nanostructure was synthesized by a facile hydrothermal method. The prepared material was characterized by FTIR spectroscopy, XRD, SEM, EDX, TGA, and Raman spectroscopy, which confirmed the formation of nanostructured material. Subsequently, the prepared nanoparticles (NPs) were capped with 1-H-3-methylimidazolium acetate ionic liquid (IL) to achieve its deagglomeration and functionalization. A new colorimetric sensing probe was prepared for the detection of H(2)O(2) based on ionic liquid-capped TiO(2) nanoparticles (TiO(2)/IL) and 3,3′,5,5′-tetramethylbenzidine (TMB) dye, which acts as an oxidative chromogenic substrate. H(2)O(2) reacts with TMB, in the presence of ionic liquid-coated TiO(2) NPs, to form a blue-green product. The color was visualized with the naked eye, and the colorimetric change was confirmed by a UV–vis spectrophotometer. To obtain the best response of the synthesized sensor, different parameters (time, pH, concentrations, loading of nanomaterials) were optimized. It showed a low limit of detection 8.61 × 10(–8) M, a high sensitivity of 2.86 × 10(–7) M, and a wide linear range of 1 × 10(–9)–3.6 × 10(–7) M, with a regression coefficient (R(2)) value of 0.999. The proposed sensor showed a short incubation time of 4 min. The sensing probe did not show any interference from the coexisting species. The TiO(2)/IL sensor was effectively used for finding H(2)O(2) in the urine samples of cancer patients. |
format | Online Article Text |
id | pubmed-8655897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-86558972021-12-10 Ionic-Liquid-Stabilized TiO(2) Nanostructures: A Platform for Detection of Hydrogen Peroxide Nishan, Umar Haq, Shams Ul Rahim, Abdur Asad, Muhammad Badshah, Amir Ali Shah, Azhar-ul-Haq Iqbal, Anwar Muhammad, Nawshad ACS Omega [Image: see text] Hydrogen peroxide (H(2)O(2)) acts as a signaling molecule to direct different biological processes. However, its excess amount results in oxidative stress, which causes the onset of different types of cancers. TiO(2) nanostructure was synthesized by a facile hydrothermal method. The prepared material was characterized by FTIR spectroscopy, XRD, SEM, EDX, TGA, and Raman spectroscopy, which confirmed the formation of nanostructured material. Subsequently, the prepared nanoparticles (NPs) were capped with 1-H-3-methylimidazolium acetate ionic liquid (IL) to achieve its deagglomeration and functionalization. A new colorimetric sensing probe was prepared for the detection of H(2)O(2) based on ionic liquid-capped TiO(2) nanoparticles (TiO(2)/IL) and 3,3′,5,5′-tetramethylbenzidine (TMB) dye, which acts as an oxidative chromogenic substrate. H(2)O(2) reacts with TMB, in the presence of ionic liquid-coated TiO(2) NPs, to form a blue-green product. The color was visualized with the naked eye, and the colorimetric change was confirmed by a UV–vis spectrophotometer. To obtain the best response of the synthesized sensor, different parameters (time, pH, concentrations, loading of nanomaterials) were optimized. It showed a low limit of detection 8.61 × 10(–8) M, a high sensitivity of 2.86 × 10(–7) M, and a wide linear range of 1 × 10(–9)–3.6 × 10(–7) M, with a regression coefficient (R(2)) value of 0.999. The proposed sensor showed a short incubation time of 4 min. The sensing probe did not show any interference from the coexisting species. The TiO(2)/IL sensor was effectively used for finding H(2)O(2) in the urine samples of cancer patients. American Chemical Society 2021-11-22 /pmc/articles/PMC8655897/ /pubmed/34901624 http://dx.doi.org/10.1021/acsomega.1c04548 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Nishan, Umar Haq, Shams Ul Rahim, Abdur Asad, Muhammad Badshah, Amir Ali Shah, Azhar-ul-Haq Iqbal, Anwar Muhammad, Nawshad Ionic-Liquid-Stabilized TiO(2) Nanostructures: A Platform for Detection of Hydrogen Peroxide |
title | Ionic-Liquid-Stabilized TiO(2) Nanostructures:
A Platform for Detection of Hydrogen Peroxide |
title_full | Ionic-Liquid-Stabilized TiO(2) Nanostructures:
A Platform for Detection of Hydrogen Peroxide |
title_fullStr | Ionic-Liquid-Stabilized TiO(2) Nanostructures:
A Platform for Detection of Hydrogen Peroxide |
title_full_unstemmed | Ionic-Liquid-Stabilized TiO(2) Nanostructures:
A Platform for Detection of Hydrogen Peroxide |
title_short | Ionic-Liquid-Stabilized TiO(2) Nanostructures:
A Platform for Detection of Hydrogen Peroxide |
title_sort | ionic-liquid-stabilized tio(2) nanostructures:
a platform for detection of hydrogen peroxide |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655897/ https://www.ncbi.nlm.nih.gov/pubmed/34901624 http://dx.doi.org/10.1021/acsomega.1c04548 |
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