Squarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications

[Image: see text] Hydrogels are useful platforms as three-dimensional (3D) scaffolds for cell culture, drug-release systems, and regenerative medicine applications. Here, we propose a novel chemical cross-linking approach by the use of 3,4-diethoxy-3-cyclobutene-1,2-dione or diethyl squarate for the...

Descripción completa

Detalles Bibliográficos
Autores principales: Stucchi, Simone, Colombo, Danilo, Guizzardi, Roberto, D’Aloia, Alessia, Collini, Maddalena, Bouzin, Margaux, Costa, Barbara, Ceriani, Michela, Natalello, Antonino, Pallavicini, Piersandro, Cipolla, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655982/
https://www.ncbi.nlm.nih.gov/pubmed/34806889
http://dx.doi.org/10.1021/acs.langmuir.1c02080
_version_ 1784612189563256832
author Stucchi, Simone
Colombo, Danilo
Guizzardi, Roberto
D’Aloia, Alessia
Collini, Maddalena
Bouzin, Margaux
Costa, Barbara
Ceriani, Michela
Natalello, Antonino
Pallavicini, Piersandro
Cipolla, Laura
author_facet Stucchi, Simone
Colombo, Danilo
Guizzardi, Roberto
D’Aloia, Alessia
Collini, Maddalena
Bouzin, Margaux
Costa, Barbara
Ceriani, Michela
Natalello, Antonino
Pallavicini, Piersandro
Cipolla, Laura
author_sort Stucchi, Simone
collection PubMed
description [Image: see text] Hydrogels are useful platforms as three-dimensional (3D) scaffolds for cell culture, drug-release systems, and regenerative medicine applications. Here, we propose a novel chemical cross-linking approach by the use of 3,4-diethoxy-3-cyclobutene-1,2-dione or diethyl squarate for the preparation of 5 and 10% w/v gelatin-based hydrogels. Hydrogels showed good swelling properties, and the 5% gelatin-based hydrogel proved suitable as a 3D cell culture scaffold for the chondrocyte cell line C28/I2. In addition, diffusion properties of different sized molecules inside the hydrogel were determined.
format Online
Article
Text
id pubmed-8655982
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-86559822021-12-09 Squarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications Stucchi, Simone Colombo, Danilo Guizzardi, Roberto D’Aloia, Alessia Collini, Maddalena Bouzin, Margaux Costa, Barbara Ceriani, Michela Natalello, Antonino Pallavicini, Piersandro Cipolla, Laura Langmuir [Image: see text] Hydrogels are useful platforms as three-dimensional (3D) scaffolds for cell culture, drug-release systems, and regenerative medicine applications. Here, we propose a novel chemical cross-linking approach by the use of 3,4-diethoxy-3-cyclobutene-1,2-dione or diethyl squarate for the preparation of 5 and 10% w/v gelatin-based hydrogels. Hydrogels showed good swelling properties, and the 5% gelatin-based hydrogel proved suitable as a 3D cell culture scaffold for the chondrocyte cell line C28/I2. In addition, diffusion properties of different sized molecules inside the hydrogel were determined. American Chemical Society 2021-11-22 2021-12-07 /pmc/articles/PMC8655982/ /pubmed/34806889 http://dx.doi.org/10.1021/acs.langmuir.1c02080 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Stucchi, Simone
Colombo, Danilo
Guizzardi, Roberto
D’Aloia, Alessia
Collini, Maddalena
Bouzin, Margaux
Costa, Barbara
Ceriani, Michela
Natalello, Antonino
Pallavicini, Piersandro
Cipolla, Laura
Squarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications
title Squarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications
title_full Squarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications
title_fullStr Squarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications
title_full_unstemmed Squarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications
title_short Squarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications
title_sort squarate cross-linked gelatin hydrogels as three-dimensional scaffolds for biomedical applications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655982/
https://www.ncbi.nlm.nih.gov/pubmed/34806889
http://dx.doi.org/10.1021/acs.langmuir.1c02080
work_keys_str_mv AT stucchisimone squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT colombodanilo squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT guizzardiroberto squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT daloiaalessia squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT collinimaddalena squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT bouzinmargaux squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT costabarbara squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT cerianimichela squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT natalelloantonino squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT pallavicinipiersandro squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications
AT cipollalaura squaratecrosslinkedgelatinhydrogelsasthreedimensionalscaffoldsforbiomedicalapplications

Ejemplares similares