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High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma

Background: Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. MFH often relapses locally after the curettage is related to the residual cancer stem cells (CSCs). Currently, the dysregulation of microRNA (miRNA) has been found to be clo...

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Autores principales: Li, Dejian, Zhao, Kai, Zhao, Ziwen, Jiang, Bo, Gong, Xianxu, Zhang, Yan, Guo, Yingqi, Xiao, Han, Wang, Ye, Liu, Hui, Yi, Chengqing, Gu, Wenguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8656228/
https://www.ncbi.nlm.nih.gov/pubmed/34900997
http://dx.doi.org/10.3389/fcell.2021.751833
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author Li, Dejian
Zhao, Kai
Zhao, Ziwen
Jiang, Bo
Gong, Xianxu
Zhang, Yan
Guo, Yingqi
Xiao, Han
Wang, Ye
Liu, Hui
Yi, Chengqing
Gu, Wenguang
author_facet Li, Dejian
Zhao, Kai
Zhao, Ziwen
Jiang, Bo
Gong, Xianxu
Zhang, Yan
Guo, Yingqi
Xiao, Han
Wang, Ye
Liu, Hui
Yi, Chengqing
Gu, Wenguang
author_sort Li, Dejian
collection PubMed
description Background: Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. MFH often relapses locally after the curettage is related to the residual cancer stem cells (CSCs). Currently, the dysregulation of microRNA (miRNA) has been found to be closely related to the recurrence of CSCs. However, whether dysregulations of miRNAs exist in MFH, CSCs remained unknown. Methods: In this study, miRNAs in MFH CSCs and MFH common cells were examined by gene probe. Then, target genes and their functions involved in the signal pathway were predicted by the relevant database. Finally, the miRNAs’ target regulatory network was constructed. Furthermore, the miRNAs and target genes were identified by quantitative polymerase chain reaction, whereas miRNA analogs and antagonists were transfected in tumor cells to investigate cell proliferation ability further. Results: Results showed that a total of 47 miRNAs were found, including 16 that were upregulated and 31 that were downregulated. The screened differential miRNA showed a different expression in the cell resistant strains compared with the control group. Quantitative polymerase chain reaction analysis confirmed that the relative abundance of seven miRNAs and four target genes varied significantly. The encouraging issue is that we found Hsa-miR-206 significantly inhibited MFH proliferative activity. Conclusion: Hsa-miR-206 played a key role in regulating MFH CSC properties that might be a representative marker and target for the diagnosis and treatment of MFH in the future.
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spelling pubmed-86562282021-12-10 High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma Li, Dejian Zhao, Kai Zhao, Ziwen Jiang, Bo Gong, Xianxu Zhang, Yan Guo, Yingqi Xiao, Han Wang, Ye Liu, Hui Yi, Chengqing Gu, Wenguang Front Cell Dev Biol Cell and Developmental Biology Background: Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. MFH often relapses locally after the curettage is related to the residual cancer stem cells (CSCs). Currently, the dysregulation of microRNA (miRNA) has been found to be closely related to the recurrence of CSCs. However, whether dysregulations of miRNAs exist in MFH, CSCs remained unknown. Methods: In this study, miRNAs in MFH CSCs and MFH common cells were examined by gene probe. Then, target genes and their functions involved in the signal pathway were predicted by the relevant database. Finally, the miRNAs’ target regulatory network was constructed. Furthermore, the miRNAs and target genes were identified by quantitative polymerase chain reaction, whereas miRNA analogs and antagonists were transfected in tumor cells to investigate cell proliferation ability further. Results: Results showed that a total of 47 miRNAs were found, including 16 that were upregulated and 31 that were downregulated. The screened differential miRNA showed a different expression in the cell resistant strains compared with the control group. Quantitative polymerase chain reaction analysis confirmed that the relative abundance of seven miRNAs and four target genes varied significantly. The encouraging issue is that we found Hsa-miR-206 significantly inhibited MFH proliferative activity. Conclusion: Hsa-miR-206 played a key role in regulating MFH CSC properties that might be a representative marker and target for the diagnosis and treatment of MFH in the future. Frontiers Media S.A. 2021-11-25 /pmc/articles/PMC8656228/ /pubmed/34900997 http://dx.doi.org/10.3389/fcell.2021.751833 Text en Copyright © 2021 Li, Zhao, Zhao, Jiang, Gong, Zhang, Guo, Xiao, Wang, Liu, Yi and Gu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Li, Dejian
Zhao, Kai
Zhao, Ziwen
Jiang, Bo
Gong, Xianxu
Zhang, Yan
Guo, Yingqi
Xiao, Han
Wang, Ye
Liu, Hui
Yi, Chengqing
Gu, Wenguang
High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_full High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_fullStr High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_full_unstemmed High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_short High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_sort high expression microrna-206 inhibits the growth of tumor cells in human malignant fibrous histiocytoma
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8656228/
https://www.ncbi.nlm.nih.gov/pubmed/34900997
http://dx.doi.org/10.3389/fcell.2021.751833
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