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Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients

SIMPLE SUMMARY: Liquid biopsy is an easily accessible and non-invasive method to gain information about tumor diseases. The purpose of our study was to determine the value of extracellular vesicle-derived mRNAs as biomarkers for the diagnosis of gastric cancer and the response to its treatment. In a...

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Autores principales: Rhode, Philipp, Mehdorn, Matthias, Lyros, Orestis, Kahlert, Christoph, Kurth, Thomas, Venus, Tom, Schierle, Katrin, Estrela-Lopis, Irina, Jansen-Winkeln, Boris, Lordick, Florian, Gockel, Ines, Thieme, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8656496/
https://www.ncbi.nlm.nih.gov/pubmed/34885085
http://dx.doi.org/10.3390/cancers13235975
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author Rhode, Philipp
Mehdorn, Matthias
Lyros, Orestis
Kahlert, Christoph
Kurth, Thomas
Venus, Tom
Schierle, Katrin
Estrela-Lopis, Irina
Jansen-Winkeln, Boris
Lordick, Florian
Gockel, Ines
Thieme, René
author_facet Rhode, Philipp
Mehdorn, Matthias
Lyros, Orestis
Kahlert, Christoph
Kurth, Thomas
Venus, Tom
Schierle, Katrin
Estrela-Lopis, Irina
Jansen-Winkeln, Boris
Lordick, Florian
Gockel, Ines
Thieme, René
author_sort Rhode, Philipp
collection PubMed
description SIMPLE SUMMARY: Liquid biopsy is an easily accessible and non-invasive method to gain information about tumor diseases. The purpose of our study was to determine the value of extracellular vesicle-derived mRNAs as biomarkers for the diagnosis of gastric cancer and the response to its treatment. In a cohort of 87 gastric cancer patients and a control group of 14 individuals, we analyzed the absolute RNA concentration from extracellular vesicles (EV) and the relative levels of FASN, PTEN, and CD44 mRNA, and their correlation with clinico-pathological features. These correlated with treatment, tumor grading, and the pathological subtype according to Laurén’s classification. This might reflect their potential as both diagnostic and therapeutic predictors. ABSTRACT: In-depth characterization has introduced new molecular subtypes of gastric cancer (GC). To identify these, new approaches and techniques are required. Liquid biopsies are trendsetting and provide an easy and feasible method to identify and to monitor GC patients. In a prospective cohort of 87 GC patients, extracellular vesicles (EVs) were isolated from 250 µL of plasma. The total RNA was isolated with TRIZOL. The total RNA amount and the relative mRNA levels of CD44, PTEN, and FASN were measured by qRT-PCR. The isolation of EVs and their contained mRNA was possible in all 87 samples investigated. The relative mRNA levels of PTEN were higher in patients already treated by chemotherapy than in chemo-naïve patients. In patients who had undergone neoadjuvant chemotherapy followed by gastrectomy, a decrease in the total RNA amount was observed after neoadjuvant chemotherapy and gastrectomy, while FASN and CD44 mRNA levels decreased only after gastrectomy. The amount of RNA and the relative mRNA levels of FASN and CD44 in EVs were affected more significantly by chemotherapy and gastrectomy than by chemotherapy alone. Therefore, they are a potential biomarker for monitoring treatment response. Future analyses are needed to identify GC-specific key RNAs in EVs, which could be used for the diagnosis of gastric cancer patients in order to determine their molecular subtype and to accompany the therapeutic response.
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spelling pubmed-86564962021-12-10 Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients Rhode, Philipp Mehdorn, Matthias Lyros, Orestis Kahlert, Christoph Kurth, Thomas Venus, Tom Schierle, Katrin Estrela-Lopis, Irina Jansen-Winkeln, Boris Lordick, Florian Gockel, Ines Thieme, René Cancers (Basel) Article SIMPLE SUMMARY: Liquid biopsy is an easily accessible and non-invasive method to gain information about tumor diseases. The purpose of our study was to determine the value of extracellular vesicle-derived mRNAs as biomarkers for the diagnosis of gastric cancer and the response to its treatment. In a cohort of 87 gastric cancer patients and a control group of 14 individuals, we analyzed the absolute RNA concentration from extracellular vesicles (EV) and the relative levels of FASN, PTEN, and CD44 mRNA, and their correlation with clinico-pathological features. These correlated with treatment, tumor grading, and the pathological subtype according to Laurén’s classification. This might reflect their potential as both diagnostic and therapeutic predictors. ABSTRACT: In-depth characterization has introduced new molecular subtypes of gastric cancer (GC). To identify these, new approaches and techniques are required. Liquid biopsies are trendsetting and provide an easy and feasible method to identify and to monitor GC patients. In a prospective cohort of 87 GC patients, extracellular vesicles (EVs) were isolated from 250 µL of plasma. The total RNA was isolated with TRIZOL. The total RNA amount and the relative mRNA levels of CD44, PTEN, and FASN were measured by qRT-PCR. The isolation of EVs and their contained mRNA was possible in all 87 samples investigated. The relative mRNA levels of PTEN were higher in patients already treated by chemotherapy than in chemo-naïve patients. In patients who had undergone neoadjuvant chemotherapy followed by gastrectomy, a decrease in the total RNA amount was observed after neoadjuvant chemotherapy and gastrectomy, while FASN and CD44 mRNA levels decreased only after gastrectomy. The amount of RNA and the relative mRNA levels of FASN and CD44 in EVs were affected more significantly by chemotherapy and gastrectomy than by chemotherapy alone. Therefore, they are a potential biomarker for monitoring treatment response. Future analyses are needed to identify GC-specific key RNAs in EVs, which could be used for the diagnosis of gastric cancer patients in order to determine their molecular subtype and to accompany the therapeutic response. MDPI 2021-11-27 /pmc/articles/PMC8656496/ /pubmed/34885085 http://dx.doi.org/10.3390/cancers13235975 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rhode, Philipp
Mehdorn, Matthias
Lyros, Orestis
Kahlert, Christoph
Kurth, Thomas
Venus, Tom
Schierle, Katrin
Estrela-Lopis, Irina
Jansen-Winkeln, Boris
Lordick, Florian
Gockel, Ines
Thieme, René
Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients
title Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients
title_full Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients
title_fullStr Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients
title_full_unstemmed Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients
title_short Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients
title_sort characterization of total rna, cd44, fasn, and pten mrnas from extracellular vesicles as biomarkers in gastric cancer patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8656496/
https://www.ncbi.nlm.nih.gov/pubmed/34885085
http://dx.doi.org/10.3390/cancers13235975
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