Cargando…
A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis
Flowering time is one of the most important agronomic traits in wheat production. A proper flowering time might contribute to the reduction or avoidance of biotic and abiotic stresses, adjust plant architecture, and affect the yield and quality of grain. In this study, TaTOE1-B1 in wheat produced th...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657464/ https://www.ncbi.nlm.nih.gov/pubmed/34884449 http://dx.doi.org/10.3390/ijms222312645 |
_version_ | 1784612509644226560 |
---|---|
author | Song, Tianqi Yu, Yang Zhang, Mingfei Zhou, Hongwei Zhang, Shuangxing Yu, Ming Zhou, Jianfei Cheng, Jie Xiang, Jishan Yang, Songjie Zhang, Xiaoke |
author_facet | Song, Tianqi Yu, Yang Zhang, Mingfei Zhou, Hongwei Zhang, Shuangxing Yu, Ming Zhou, Jianfei Cheng, Jie Xiang, Jishan Yang, Songjie Zhang, Xiaoke |
author_sort | Song, Tianqi |
collection | PubMed |
description | Flowering time is one of the most important agronomic traits in wheat production. A proper flowering time might contribute to the reduction or avoidance of biotic and abiotic stresses, adjust plant architecture, and affect the yield and quality of grain. In this study, TaTOE1-B1 in wheat produced three transcripts (TaTOE1-B1-1, TaTOE1-B1-2, and TaTOE1-B1-3) by alternative splicing. Compared to the longest transcript, TaTOE1-B1-1, TaTOE1-B1-3 has a deletion in the sixth exon (1219–1264 bp). Under long-day conditions, the heterologous overexpression of the TaTOE1-B1-3 gene delayed flowering, prolonged the vegetative growth time, and enlarged the vegetative body of Arabidopsis, but that of TaTOE1-B1-1 did not. As typical AP2 family members, TaTOE1-B1-1 and TaTOE1-B1-3 are mainly located in the nucleus and have transcriptional activation activities; the transcriptional activation region of TaTOE1-B1-3 is located in the C-terminal. In TaTOE1-B1-3 overexpression lines, the expression of flowering-related AtFT and AtSOC1 genes is significantly downregulated. In addition, this study confirms the protein–protein interaction between TaTOE1-B1-3 and TaPIFI, which may play an important role in flowering inhibition. These results provide a theoretical basis for the precise regulation of wheat flowering time. |
format | Online Article Text |
id | pubmed-8657464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86574642021-12-10 A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis Song, Tianqi Yu, Yang Zhang, Mingfei Zhou, Hongwei Zhang, Shuangxing Yu, Ming Zhou, Jianfei Cheng, Jie Xiang, Jishan Yang, Songjie Zhang, Xiaoke Int J Mol Sci Article Flowering time is one of the most important agronomic traits in wheat production. A proper flowering time might contribute to the reduction or avoidance of biotic and abiotic stresses, adjust plant architecture, and affect the yield and quality of grain. In this study, TaTOE1-B1 in wheat produced three transcripts (TaTOE1-B1-1, TaTOE1-B1-2, and TaTOE1-B1-3) by alternative splicing. Compared to the longest transcript, TaTOE1-B1-1, TaTOE1-B1-3 has a deletion in the sixth exon (1219–1264 bp). Under long-day conditions, the heterologous overexpression of the TaTOE1-B1-3 gene delayed flowering, prolonged the vegetative growth time, and enlarged the vegetative body of Arabidopsis, but that of TaTOE1-B1-1 did not. As typical AP2 family members, TaTOE1-B1-1 and TaTOE1-B1-3 are mainly located in the nucleus and have transcriptional activation activities; the transcriptional activation region of TaTOE1-B1-3 is located in the C-terminal. In TaTOE1-B1-3 overexpression lines, the expression of flowering-related AtFT and AtSOC1 genes is significantly downregulated. In addition, this study confirms the protein–protein interaction between TaTOE1-B1-3 and TaPIFI, which may play an important role in flowering inhibition. These results provide a theoretical basis for the precise regulation of wheat flowering time. MDPI 2021-11-23 /pmc/articles/PMC8657464/ /pubmed/34884449 http://dx.doi.org/10.3390/ijms222312645 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Song, Tianqi Yu, Yang Zhang, Mingfei Zhou, Hongwei Zhang, Shuangxing Yu, Ming Zhou, Jianfei Cheng, Jie Xiang, Jishan Yang, Songjie Zhang, Xiaoke A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis |
title | A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis |
title_full | A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis |
title_fullStr | A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis |
title_full_unstemmed | A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis |
title_short | A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis |
title_sort | wheat tatoe1-b1 transcript tatoe1-b1-3 can delay the flowering time of transgenic arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657464/ https://www.ncbi.nlm.nih.gov/pubmed/34884449 http://dx.doi.org/10.3390/ijms222312645 |
work_keys_str_mv | AT songtianqi awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT yuyang awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhangmingfei awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhouhongwei awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhangshuangxing awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT yuming awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhoujianfei awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT chengjie awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT xiangjishan awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT yangsongjie awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhangxiaoke awheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT songtianqi wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT yuyang wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhangmingfei wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhouhongwei wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhangshuangxing wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT yuming wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhoujianfei wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT chengjie wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT xiangjishan wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT yangsongjie wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis AT zhangxiaoke wheattatoe1b1transcripttatoe1b13candelaythefloweringtimeoftransgenicarabidopsis |