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A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis

Flowering time is one of the most important agronomic traits in wheat production. A proper flowering time might contribute to the reduction or avoidance of biotic and abiotic stresses, adjust plant architecture, and affect the yield and quality of grain. In this study, TaTOE1-B1 in wheat produced th...

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Autores principales: Song, Tianqi, Yu, Yang, Zhang, Mingfei, Zhou, Hongwei, Zhang, Shuangxing, Yu, Ming, Zhou, Jianfei, Cheng, Jie, Xiang, Jishan, Yang, Songjie, Zhang, Xiaoke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657464/
https://www.ncbi.nlm.nih.gov/pubmed/34884449
http://dx.doi.org/10.3390/ijms222312645
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author Song, Tianqi
Yu, Yang
Zhang, Mingfei
Zhou, Hongwei
Zhang, Shuangxing
Yu, Ming
Zhou, Jianfei
Cheng, Jie
Xiang, Jishan
Yang, Songjie
Zhang, Xiaoke
author_facet Song, Tianqi
Yu, Yang
Zhang, Mingfei
Zhou, Hongwei
Zhang, Shuangxing
Yu, Ming
Zhou, Jianfei
Cheng, Jie
Xiang, Jishan
Yang, Songjie
Zhang, Xiaoke
author_sort Song, Tianqi
collection PubMed
description Flowering time is one of the most important agronomic traits in wheat production. A proper flowering time might contribute to the reduction or avoidance of biotic and abiotic stresses, adjust plant architecture, and affect the yield and quality of grain. In this study, TaTOE1-B1 in wheat produced three transcripts (TaTOE1-B1-1, TaTOE1-B1-2, and TaTOE1-B1-3) by alternative splicing. Compared to the longest transcript, TaTOE1-B1-1, TaTOE1-B1-3 has a deletion in the sixth exon (1219–1264 bp). Under long-day conditions, the heterologous overexpression of the TaTOE1-B1-3 gene delayed flowering, prolonged the vegetative growth time, and enlarged the vegetative body of Arabidopsis, but that of TaTOE1-B1-1 did not. As typical AP2 family members, TaTOE1-B1-1 and TaTOE1-B1-3 are mainly located in the nucleus and have transcriptional activation activities; the transcriptional activation region of TaTOE1-B1-3 is located in the C-terminal. In TaTOE1-B1-3 overexpression lines, the expression of flowering-related AtFT and AtSOC1 genes is significantly downregulated. In addition, this study confirms the protein–protein interaction between TaTOE1-B1-3 and TaPIFI, which may play an important role in flowering inhibition. These results provide a theoretical basis for the precise regulation of wheat flowering time.
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spelling pubmed-86574642021-12-10 A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis Song, Tianqi Yu, Yang Zhang, Mingfei Zhou, Hongwei Zhang, Shuangxing Yu, Ming Zhou, Jianfei Cheng, Jie Xiang, Jishan Yang, Songjie Zhang, Xiaoke Int J Mol Sci Article Flowering time is one of the most important agronomic traits in wheat production. A proper flowering time might contribute to the reduction or avoidance of biotic and abiotic stresses, adjust plant architecture, and affect the yield and quality of grain. In this study, TaTOE1-B1 in wheat produced three transcripts (TaTOE1-B1-1, TaTOE1-B1-2, and TaTOE1-B1-3) by alternative splicing. Compared to the longest transcript, TaTOE1-B1-1, TaTOE1-B1-3 has a deletion in the sixth exon (1219–1264 bp). Under long-day conditions, the heterologous overexpression of the TaTOE1-B1-3 gene delayed flowering, prolonged the vegetative growth time, and enlarged the vegetative body of Arabidopsis, but that of TaTOE1-B1-1 did not. As typical AP2 family members, TaTOE1-B1-1 and TaTOE1-B1-3 are mainly located in the nucleus and have transcriptional activation activities; the transcriptional activation region of TaTOE1-B1-3 is located in the C-terminal. In TaTOE1-B1-3 overexpression lines, the expression of flowering-related AtFT and AtSOC1 genes is significantly downregulated. In addition, this study confirms the protein–protein interaction between TaTOE1-B1-3 and TaPIFI, which may play an important role in flowering inhibition. These results provide a theoretical basis for the precise regulation of wheat flowering time. MDPI 2021-11-23 /pmc/articles/PMC8657464/ /pubmed/34884449 http://dx.doi.org/10.3390/ijms222312645 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Tianqi
Yu, Yang
Zhang, Mingfei
Zhou, Hongwei
Zhang, Shuangxing
Yu, Ming
Zhou, Jianfei
Cheng, Jie
Xiang, Jishan
Yang, Songjie
Zhang, Xiaoke
A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis
title A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis
title_full A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis
title_fullStr A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis
title_full_unstemmed A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis
title_short A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis
title_sort wheat tatoe1-b1 transcript tatoe1-b1-3 can delay the flowering time of transgenic arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657464/
https://www.ncbi.nlm.nih.gov/pubmed/34884449
http://dx.doi.org/10.3390/ijms222312645
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