Cargando…
Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins
Sperm peroxiredoxins (PRDXs) are moonlighting proteins which, in addition to their antioxidant activity, also act as redox signal transducers through PRDX-induced oxidative post-translational modifications of proteins (oxPTMs). Despite extensive knowledge on the antioxidant activity of PRDXs, the me...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657687/ https://www.ncbi.nlm.nih.gov/pubmed/34884692 http://dx.doi.org/10.3390/ijms222312888 |
_version_ | 1784612558385184768 |
---|---|
author | Mostek-Majewska, Agnieszka Janta, Anna Majewska, Anna Ciereszko, Andrzej |
author_facet | Mostek-Majewska, Agnieszka Janta, Anna Majewska, Anna Ciereszko, Andrzej |
author_sort | Mostek-Majewska, Agnieszka |
collection | PubMed |
description | Sperm peroxiredoxins (PRDXs) are moonlighting proteins which, in addition to their antioxidant activity, also act as redox signal transducers through PRDX-induced oxidative post-translational modifications of proteins (oxPTMs). Despite extensive knowledge on the antioxidant activity of PRDXs, the mechanisms related to PRDX-mediated oxPTMs are poorly understood. The present study aimed to investigate the effect of bull sperm 2-Cys PRDX inhibition by Conoidin A on changes in oxPTM levels under control and oxidative stress conditions. The results showed that a group of sperm mitochondrial (LDHAL6B, CS, ACO2, SDHA, ACAPM) and actin cytoskeleton proteins (CAPZB, ALDOA, CCIN) is oxidized due to the action of 2-Cys PRDXs under control conditions. In turn, under oxidative stress conditions, 2-Cys PRDX activity seems to be focused on antioxidant function protecting glycolytic, TCA pathway, and respiratory chain enzymes; chaperones; and sperm axonemal tubulins from oxidative damage. Interestingly, the inhibition of PRDX resulted in oxidation of a group of rate-limiting glycolytic proteins, which is known to trigger the switching of glucose metabolism from glycolysis to pentose phosphate pathway (PPP). The obtained results are expected to broaden the knowledge of the potential role of bull sperm 2-Cys in both redox signal transmission and antioxidant activity. |
format | Online Article Text |
id | pubmed-8657687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86576872021-12-10 Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins Mostek-Majewska, Agnieszka Janta, Anna Majewska, Anna Ciereszko, Andrzej Int J Mol Sci Article Sperm peroxiredoxins (PRDXs) are moonlighting proteins which, in addition to their antioxidant activity, also act as redox signal transducers through PRDX-induced oxidative post-translational modifications of proteins (oxPTMs). Despite extensive knowledge on the antioxidant activity of PRDXs, the mechanisms related to PRDX-mediated oxPTMs are poorly understood. The present study aimed to investigate the effect of bull sperm 2-Cys PRDX inhibition by Conoidin A on changes in oxPTM levels under control and oxidative stress conditions. The results showed that a group of sperm mitochondrial (LDHAL6B, CS, ACO2, SDHA, ACAPM) and actin cytoskeleton proteins (CAPZB, ALDOA, CCIN) is oxidized due to the action of 2-Cys PRDXs under control conditions. In turn, under oxidative stress conditions, 2-Cys PRDX activity seems to be focused on antioxidant function protecting glycolytic, TCA pathway, and respiratory chain enzymes; chaperones; and sperm axonemal tubulins from oxidative damage. Interestingly, the inhibition of PRDX resulted in oxidation of a group of rate-limiting glycolytic proteins, which is known to trigger the switching of glucose metabolism from glycolysis to pentose phosphate pathway (PPP). The obtained results are expected to broaden the knowledge of the potential role of bull sperm 2-Cys in both redox signal transmission and antioxidant activity. MDPI 2021-11-28 /pmc/articles/PMC8657687/ /pubmed/34884692 http://dx.doi.org/10.3390/ijms222312888 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mostek-Majewska, Agnieszka Janta, Anna Majewska, Anna Ciereszko, Andrzej Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins |
title | Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins |
title_full | Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins |
title_fullStr | Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins |
title_full_unstemmed | Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins |
title_short | Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins |
title_sort | effect of 2-cys peroxiredoxins inhibition on redox modifications of bull sperm proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657687/ https://www.ncbi.nlm.nih.gov/pubmed/34884692 http://dx.doi.org/10.3390/ijms222312888 |
work_keys_str_mv | AT mostekmajewskaagnieszka effectof2cysperoxiredoxinsinhibitiononredoxmodificationsofbullspermproteins AT jantaanna effectof2cysperoxiredoxinsinhibitiononredoxmodificationsofbullspermproteins AT majewskaanna effectof2cysperoxiredoxinsinhibitiononredoxmodificationsofbullspermproteins AT ciereszkoandrzej effectof2cysperoxiredoxinsinhibitiononredoxmodificationsofbullspermproteins |