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The Role of Pectobacterium atrosepticum Exopolysaccharides in Plant–Pathogen Interactions

The phytopathogenic bacterium Pectobacterium atrosepticum (Pba), one of the members of the soft rot Pectobacteriaceae, forms biofilm-like structures known as bacterial emboli when colonizing the primary xylem vessels of the host plants. The initial extracellular matrix of the bacterial emboli is com...

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Autores principales: Islamov, Bakhtiyar, Petrova, Olga, Mikshina, Polina, Kadyirov, Aidar, Vorob’ev, Vladimir, Gogolev, Yuri, Gorshkov, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657720/
https://www.ncbi.nlm.nih.gov/pubmed/34884586
http://dx.doi.org/10.3390/ijms222312781
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author Islamov, Bakhtiyar
Petrova, Olga
Mikshina, Polina
Kadyirov, Aidar
Vorob’ev, Vladimir
Gogolev, Yuri
Gorshkov, Vladimir
author_facet Islamov, Bakhtiyar
Petrova, Olga
Mikshina, Polina
Kadyirov, Aidar
Vorob’ev, Vladimir
Gogolev, Yuri
Gorshkov, Vladimir
author_sort Islamov, Bakhtiyar
collection PubMed
description The phytopathogenic bacterium Pectobacterium atrosepticum (Pba), one of the members of the soft rot Pectobacteriaceae, forms biofilm-like structures known as bacterial emboli when colonizing the primary xylem vessels of the host plants. The initial extracellular matrix of the bacterial emboli is composed of the host plant’s pectic polysaccharides, which are gradually substituted by the Pba-produced exopolysaccharides (Pba EPS) as the bacterial emboli “mature”. No information about the properties of Pba EPS and their possible roles in Pba-plant interactions has so far been obtained. We have shown that Pba EPS possess physical properties that can promote the maintenance of the structural integrity of bacterial emboli. These polymers increase the viscosity of liquids and form large supramolecular aggregates. The formation of Pba EPS aggregates is provided (at least partly) by the acetyl groups of the Pba EPS molecules. Besides, Pba EPS scavenge reactive oxygen species (ROS), the accumulation of which is known to be associated with the formation of bacterial emboli. In addition, Pba EPS act as suppressors of the quantitative immunity of plants, repressing PAMP-induced reactions; this property is partly lost in the deacetylated form of Pba EPS. Overall, our study shows that Pba EPS play structural, protective, and immunosuppressive roles during Pba–plant interactions and thus should be considered as virulence factors of these bacteria.
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spelling pubmed-86577202021-12-10 The Role of Pectobacterium atrosepticum Exopolysaccharides in Plant–Pathogen Interactions Islamov, Bakhtiyar Petrova, Olga Mikshina, Polina Kadyirov, Aidar Vorob’ev, Vladimir Gogolev, Yuri Gorshkov, Vladimir Int J Mol Sci Article The phytopathogenic bacterium Pectobacterium atrosepticum (Pba), one of the members of the soft rot Pectobacteriaceae, forms biofilm-like structures known as bacterial emboli when colonizing the primary xylem vessels of the host plants. The initial extracellular matrix of the bacterial emboli is composed of the host plant’s pectic polysaccharides, which are gradually substituted by the Pba-produced exopolysaccharides (Pba EPS) as the bacterial emboli “mature”. No information about the properties of Pba EPS and their possible roles in Pba-plant interactions has so far been obtained. We have shown that Pba EPS possess physical properties that can promote the maintenance of the structural integrity of bacterial emboli. These polymers increase the viscosity of liquids and form large supramolecular aggregates. The formation of Pba EPS aggregates is provided (at least partly) by the acetyl groups of the Pba EPS molecules. Besides, Pba EPS scavenge reactive oxygen species (ROS), the accumulation of which is known to be associated with the formation of bacterial emboli. In addition, Pba EPS act as suppressors of the quantitative immunity of plants, repressing PAMP-induced reactions; this property is partly lost in the deacetylated form of Pba EPS. Overall, our study shows that Pba EPS play structural, protective, and immunosuppressive roles during Pba–plant interactions and thus should be considered as virulence factors of these bacteria. MDPI 2021-11-26 /pmc/articles/PMC8657720/ /pubmed/34884586 http://dx.doi.org/10.3390/ijms222312781 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Islamov, Bakhtiyar
Petrova, Olga
Mikshina, Polina
Kadyirov, Aidar
Vorob’ev, Vladimir
Gogolev, Yuri
Gorshkov, Vladimir
The Role of Pectobacterium atrosepticum Exopolysaccharides in Plant–Pathogen Interactions
title The Role of Pectobacterium atrosepticum Exopolysaccharides in Plant–Pathogen Interactions
title_full The Role of Pectobacterium atrosepticum Exopolysaccharides in Plant–Pathogen Interactions
title_fullStr The Role of Pectobacterium atrosepticum Exopolysaccharides in Plant–Pathogen Interactions
title_full_unstemmed The Role of Pectobacterium atrosepticum Exopolysaccharides in Plant–Pathogen Interactions
title_short The Role of Pectobacterium atrosepticum Exopolysaccharides in Plant–Pathogen Interactions
title_sort role of pectobacterium atrosepticum exopolysaccharides in plant–pathogen interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657720/
https://www.ncbi.nlm.nih.gov/pubmed/34884586
http://dx.doi.org/10.3390/ijms222312781
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