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Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice

Streptococcus pneumoniae is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of S. pneumoniae and Porphyromonas gingivalis, a periodontopathic bacter...

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Autores principales: Okabe, Teppei, Kamiya, Yosuke, Kikuchi, Takeshi, Goto, Hisashi, Umemura, Masayuki, Suzuki, Yuki, Sugita, Yoshihiko, Naiki, Yoshikazu, Hasegawa, Yoshiaki, Hayashi, Jun-ichiro, Kawamura, Shotaro, Sawada, Noritaka, Takayanagi, Yuhei, Fujimura, Takeki, Higuchi, Naoya, Mitani, Akio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657795/
https://www.ncbi.nlm.nih.gov/pubmed/34884507
http://dx.doi.org/10.3390/ijms222312704
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author Okabe, Teppei
Kamiya, Yosuke
Kikuchi, Takeshi
Goto, Hisashi
Umemura, Masayuki
Suzuki, Yuki
Sugita, Yoshihiko
Naiki, Yoshikazu
Hasegawa, Yoshiaki
Hayashi, Jun-ichiro
Kawamura, Shotaro
Sawada, Noritaka
Takayanagi, Yuhei
Fujimura, Takeki
Higuchi, Naoya
Mitani, Akio
author_facet Okabe, Teppei
Kamiya, Yosuke
Kikuchi, Takeshi
Goto, Hisashi
Umemura, Masayuki
Suzuki, Yuki
Sugita, Yoshihiko
Naiki, Yoshikazu
Hasegawa, Yoshiaki
Hayashi, Jun-ichiro
Kawamura, Shotaro
Sawada, Noritaka
Takayanagi, Yuhei
Fujimura, Takeki
Higuchi, Naoya
Mitani, Akio
author_sort Okabe, Teppei
collection PubMed
description Streptococcus pneumoniae is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of S. pneumoniae and Porphyromonas gingivalis, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether P. gingivalis accelerates pneumococcal infections, we tested the effects of inoculating P. gingivalis culture supernatant (PgSup) into S. pneumoniae-infected mice. Mice were intratracheally injected with S. pneumoniae and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in S. pneumoniae-infected mice and S. pnemoniae and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of S. pneumoniae-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with S. pneumoniae infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by S. pneumoniae, suggesting that virulence factors produced by P. gingivalis are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia.
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spelling pubmed-86577952021-12-10 Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice Okabe, Teppei Kamiya, Yosuke Kikuchi, Takeshi Goto, Hisashi Umemura, Masayuki Suzuki, Yuki Sugita, Yoshihiko Naiki, Yoshikazu Hasegawa, Yoshiaki Hayashi, Jun-ichiro Kawamura, Shotaro Sawada, Noritaka Takayanagi, Yuhei Fujimura, Takeki Higuchi, Naoya Mitani, Akio Int J Mol Sci Article Streptococcus pneumoniae is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of S. pneumoniae and Porphyromonas gingivalis, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether P. gingivalis accelerates pneumococcal infections, we tested the effects of inoculating P. gingivalis culture supernatant (PgSup) into S. pneumoniae-infected mice. Mice were intratracheally injected with S. pneumoniae and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in S. pneumoniae-infected mice and S. pnemoniae and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of S. pneumoniae-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with S. pneumoniae infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by S. pneumoniae, suggesting that virulence factors produced by P. gingivalis are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia. MDPI 2021-11-24 /pmc/articles/PMC8657795/ /pubmed/34884507 http://dx.doi.org/10.3390/ijms222312704 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Okabe, Teppei
Kamiya, Yosuke
Kikuchi, Takeshi
Goto, Hisashi
Umemura, Masayuki
Suzuki, Yuki
Sugita, Yoshihiko
Naiki, Yoshikazu
Hasegawa, Yoshiaki
Hayashi, Jun-ichiro
Kawamura, Shotaro
Sawada, Noritaka
Takayanagi, Yuhei
Fujimura, Takeki
Higuchi, Naoya
Mitani, Akio
Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice
title Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice
title_full Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice
title_fullStr Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice
title_full_unstemmed Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice
title_short Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice
title_sort porphyromonas gingivalis components/secretions synergistically enhance pneumonia caused by streptococcus pneumoniae in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657795/
https://www.ncbi.nlm.nih.gov/pubmed/34884507
http://dx.doi.org/10.3390/ijms222312704
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