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Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice
Streptococcus pneumoniae is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of S. pneumoniae and Porphyromonas gingivalis, a periodontopathic bacter...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657795/ https://www.ncbi.nlm.nih.gov/pubmed/34884507 http://dx.doi.org/10.3390/ijms222312704 |
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author | Okabe, Teppei Kamiya, Yosuke Kikuchi, Takeshi Goto, Hisashi Umemura, Masayuki Suzuki, Yuki Sugita, Yoshihiko Naiki, Yoshikazu Hasegawa, Yoshiaki Hayashi, Jun-ichiro Kawamura, Shotaro Sawada, Noritaka Takayanagi, Yuhei Fujimura, Takeki Higuchi, Naoya Mitani, Akio |
author_facet | Okabe, Teppei Kamiya, Yosuke Kikuchi, Takeshi Goto, Hisashi Umemura, Masayuki Suzuki, Yuki Sugita, Yoshihiko Naiki, Yoshikazu Hasegawa, Yoshiaki Hayashi, Jun-ichiro Kawamura, Shotaro Sawada, Noritaka Takayanagi, Yuhei Fujimura, Takeki Higuchi, Naoya Mitani, Akio |
author_sort | Okabe, Teppei |
collection | PubMed |
description | Streptococcus pneumoniae is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of S. pneumoniae and Porphyromonas gingivalis, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether P. gingivalis accelerates pneumococcal infections, we tested the effects of inoculating P. gingivalis culture supernatant (PgSup) into S. pneumoniae-infected mice. Mice were intratracheally injected with S. pneumoniae and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in S. pneumoniae-infected mice and S. pnemoniae and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of S. pneumoniae-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with S. pneumoniae infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by S. pneumoniae, suggesting that virulence factors produced by P. gingivalis are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia. |
format | Online Article Text |
id | pubmed-8657795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86577952021-12-10 Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice Okabe, Teppei Kamiya, Yosuke Kikuchi, Takeshi Goto, Hisashi Umemura, Masayuki Suzuki, Yuki Sugita, Yoshihiko Naiki, Yoshikazu Hasegawa, Yoshiaki Hayashi, Jun-ichiro Kawamura, Shotaro Sawada, Noritaka Takayanagi, Yuhei Fujimura, Takeki Higuchi, Naoya Mitani, Akio Int J Mol Sci Article Streptococcus pneumoniae is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of S. pneumoniae and Porphyromonas gingivalis, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether P. gingivalis accelerates pneumococcal infections, we tested the effects of inoculating P. gingivalis culture supernatant (PgSup) into S. pneumoniae-infected mice. Mice were intratracheally injected with S. pneumoniae and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in S. pneumoniae-infected mice and S. pnemoniae and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of S. pneumoniae-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with S. pneumoniae infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by S. pneumoniae, suggesting that virulence factors produced by P. gingivalis are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia. MDPI 2021-11-24 /pmc/articles/PMC8657795/ /pubmed/34884507 http://dx.doi.org/10.3390/ijms222312704 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Okabe, Teppei Kamiya, Yosuke Kikuchi, Takeshi Goto, Hisashi Umemura, Masayuki Suzuki, Yuki Sugita, Yoshihiko Naiki, Yoshikazu Hasegawa, Yoshiaki Hayashi, Jun-ichiro Kawamura, Shotaro Sawada, Noritaka Takayanagi, Yuhei Fujimura, Takeki Higuchi, Naoya Mitani, Akio Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice |
title | Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice |
title_full | Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice |
title_fullStr | Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice |
title_full_unstemmed | Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice |
title_short | Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice |
title_sort | porphyromonas gingivalis components/secretions synergistically enhance pneumonia caused by streptococcus pneumoniae in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657795/ https://www.ncbi.nlm.nih.gov/pubmed/34884507 http://dx.doi.org/10.3390/ijms222312704 |
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