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Subzero Nonfreezing Hypothermia with Insect Antifreeze Protein Dramatically Improves Survival Rate of Mammalian Cells
Cells for therapeutic use are often preserved at +4 °C, and the storage period is generally limited to 2–3 days. Here, we report that the survival rate (%) of mammalian cells is improved to 10–20 days when they are preserved with a subzero supercooled solution containing the antifreeze protein (AFP)...
Autores principales: | Yamauchi, Akari, Miura, Ai, Kondo, Hidemasa, Arai, Tatsuya, Sasaki, Yuji C., Tsuda, Sakae |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657916/ https://www.ncbi.nlm.nih.gov/pubmed/34884483 http://dx.doi.org/10.3390/ijms222312680 |
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