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An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera

Polycythemia Vera (PV) is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. Chronic inflammation is commonly observed in myeloproliferative neoplasms including PV. The inflammatory network includes the extracellular vesicles (EVs), which play a role in...

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Autores principales: Barone, Monica, Barone, Martina, Ricci, Francesca, Auteri, Giuseppe, Corradi, Giulia, Fabbri, Francesco, Papa, Valentina, Bandini, Erika, Cenacchi, Giovanna, Tazzari, Pier Luigi, Vianelli, Nicola, Turroni, Silvia, Cavo, Michele, Palandri, Francesca, Candela, Marco, Catani, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657945/
https://www.ncbi.nlm.nih.gov/pubmed/34900671
http://dx.doi.org/10.3389/fonc.2021.715217
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author Barone, Monica
Barone, Martina
Ricci, Francesca
Auteri, Giuseppe
Corradi, Giulia
Fabbri, Francesco
Papa, Valentina
Bandini, Erika
Cenacchi, Giovanna
Tazzari, Pier Luigi
Vianelli, Nicola
Turroni, Silvia
Cavo, Michele
Palandri, Francesca
Candela, Marco
Catani, Lucia
author_facet Barone, Monica
Barone, Martina
Ricci, Francesca
Auteri, Giuseppe
Corradi, Giulia
Fabbri, Francesco
Papa, Valentina
Bandini, Erika
Cenacchi, Giovanna
Tazzari, Pier Luigi
Vianelli, Nicola
Turroni, Silvia
Cavo, Michele
Palandri, Francesca
Candela, Marco
Catani, Lucia
author_sort Barone, Monica
collection PubMed
description Polycythemia Vera (PV) is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. Chronic inflammation is commonly observed in myeloproliferative neoplasms including PV. The inflammatory network includes the extracellular vesicles (EVs), which play a role in cell-cell communication. Recent evidence points to circulating microbial components/microbes as potential players in hemopoiesis regulation. To address the role of EVs in PV, here we investigated phenotype and microbial DNA cargo of circulating EVs through multidimensional analysis. Peripheral blood and feces were collected from PV patients (n=38) and healthy donors (n=30). Circulating megakaryocyte (MK)- and platelet (PLT)-derived EVs were analyzed by flow cytometry. After microbial DNA extraction from feces and isolated EVs, the 16S rDNA V3-V4 region was sequenced. We found that the proportion of circulating MK-derived EVs was significantly decreased in PV patients as compared with the healthy donors. By contrast, the proportion of the PLT-derived EVs was increased. Interestingly, PV was also associated with a microbial DNA signature of the isolated EVs with higher diversity and distinct microbial composition than the healthy counterparts. Of note, increased proportion of isolated lipopolysaccharide-associated EVs has been demonstrated in PV patients. Conversely, the gut microbiome profile failed to identify a distinct layout between PV patients and healthy donors. In conclusion, PV is associated with circulating EVs harbouring abnormal phenotype and dysbiosis signature with a potential role in the (inflammatory) pathogenesis of the disease.
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spelling pubmed-86579452021-12-10 An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera Barone, Monica Barone, Martina Ricci, Francesca Auteri, Giuseppe Corradi, Giulia Fabbri, Francesco Papa, Valentina Bandini, Erika Cenacchi, Giovanna Tazzari, Pier Luigi Vianelli, Nicola Turroni, Silvia Cavo, Michele Palandri, Francesca Candela, Marco Catani, Lucia Front Oncol Oncology Polycythemia Vera (PV) is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. Chronic inflammation is commonly observed in myeloproliferative neoplasms including PV. The inflammatory network includes the extracellular vesicles (EVs), which play a role in cell-cell communication. Recent evidence points to circulating microbial components/microbes as potential players in hemopoiesis regulation. To address the role of EVs in PV, here we investigated phenotype and microbial DNA cargo of circulating EVs through multidimensional analysis. Peripheral blood and feces were collected from PV patients (n=38) and healthy donors (n=30). Circulating megakaryocyte (MK)- and platelet (PLT)-derived EVs were analyzed by flow cytometry. After microbial DNA extraction from feces and isolated EVs, the 16S rDNA V3-V4 region was sequenced. We found that the proportion of circulating MK-derived EVs was significantly decreased in PV patients as compared with the healthy donors. By contrast, the proportion of the PLT-derived EVs was increased. Interestingly, PV was also associated with a microbial DNA signature of the isolated EVs with higher diversity and distinct microbial composition than the healthy counterparts. Of note, increased proportion of isolated lipopolysaccharide-associated EVs has been demonstrated in PV patients. Conversely, the gut microbiome profile failed to identify a distinct layout between PV patients and healthy donors. In conclusion, PV is associated with circulating EVs harbouring abnormal phenotype and dysbiosis signature with a potential role in the (inflammatory) pathogenesis of the disease. Frontiers Media S.A. 2021-11-25 /pmc/articles/PMC8657945/ /pubmed/34900671 http://dx.doi.org/10.3389/fonc.2021.715217 Text en Copyright © 2021 Barone, Barone, Ricci, Auteri, Corradi, Fabbri, Papa, Bandini, Cenacchi, Tazzari, Vianelli, Turroni, Cavo, Palandri, Candela and Catani https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Barone, Monica
Barone, Martina
Ricci, Francesca
Auteri, Giuseppe
Corradi, Giulia
Fabbri, Francesco
Papa, Valentina
Bandini, Erika
Cenacchi, Giovanna
Tazzari, Pier Luigi
Vianelli, Nicola
Turroni, Silvia
Cavo, Michele
Palandri, Francesca
Candela, Marco
Catani, Lucia
An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_full An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_fullStr An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_full_unstemmed An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_short An Abnormal Host/Microbiomes Signature of Plasma-Derived Extracellular Vesicles Is Associated to Polycythemia Vera
title_sort abnormal host/microbiomes signature of plasma-derived extracellular vesicles is associated to polycythemia vera
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657945/
https://www.ncbi.nlm.nih.gov/pubmed/34900671
http://dx.doi.org/10.3389/fonc.2021.715217
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