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Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco
Cytochrome c3 (uranyl reductase) from Desulfovibrio vulgaris can reduce uranium in bacterial cells and in cell-free systems. This gene was introduced in tobacco under control of the RbcS promoter, and the resulting transgenic plants accumulated uranium when grown on a uranyl ion containing medium. T...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657950/ https://www.ncbi.nlm.nih.gov/pubmed/34884428 http://dx.doi.org/10.3390/ijms222312622 |
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author | Beliaev, Denis V. Tereshonok, Dmitry V. Lunkova, Nina F. Baranova, Ekaterina N. Osipova, Ekaterina S. Lisovskii, Stepan V. Raldugina, Galina N. Kuznetsov, Vladimir V. |
author_facet | Beliaev, Denis V. Tereshonok, Dmitry V. Lunkova, Nina F. Baranova, Ekaterina N. Osipova, Ekaterina S. Lisovskii, Stepan V. Raldugina, Galina N. Kuznetsov, Vladimir V. |
author_sort | Beliaev, Denis V. |
collection | PubMed |
description | Cytochrome c3 (uranyl reductase) from Desulfovibrio vulgaris can reduce uranium in bacterial cells and in cell-free systems. This gene was introduced in tobacco under control of the RbcS promoter, and the resulting transgenic plants accumulated uranium when grown on a uranyl ion containing medium. The uptaken uranium was detected by EM in chloroplasts. In the presence of uranyl ions in sublethal concentration, the transgenic plants grew phenotypically normal while the control plants’ development was impaired. The data on uranium oxidation state in the transgenic plants and the possible uses of uranium hyperaccumulation by plants for environmental cleanup are discussed. |
format | Online Article Text |
id | pubmed-8657950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86579502021-12-10 Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco Beliaev, Denis V. Tereshonok, Dmitry V. Lunkova, Nina F. Baranova, Ekaterina N. Osipova, Ekaterina S. Lisovskii, Stepan V. Raldugina, Galina N. Kuznetsov, Vladimir V. Int J Mol Sci Article Cytochrome c3 (uranyl reductase) from Desulfovibrio vulgaris can reduce uranium in bacterial cells and in cell-free systems. This gene was introduced in tobacco under control of the RbcS promoter, and the resulting transgenic plants accumulated uranium when grown on a uranyl ion containing medium. The uptaken uranium was detected by EM in chloroplasts. In the presence of uranyl ions in sublethal concentration, the transgenic plants grew phenotypically normal while the control plants’ development was impaired. The data on uranium oxidation state in the transgenic plants and the possible uses of uranium hyperaccumulation by plants for environmental cleanup are discussed. MDPI 2021-11-23 /pmc/articles/PMC8657950/ /pubmed/34884428 http://dx.doi.org/10.3390/ijms222312622 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Beliaev, Denis V. Tereshonok, Dmitry V. Lunkova, Nina F. Baranova, Ekaterina N. Osipova, Ekaterina S. Lisovskii, Stepan V. Raldugina, Galina N. Kuznetsov, Vladimir V. Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco |
title | Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco |
title_full | Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco |
title_fullStr | Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco |
title_full_unstemmed | Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco |
title_short | Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco |
title_sort | expression of cytochrome c3 from desulfovibrio vulgaris in plant leaves enhances uranium uptake and tolerance of tobacco |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657950/ https://www.ncbi.nlm.nih.gov/pubmed/34884428 http://dx.doi.org/10.3390/ijms222312622 |
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