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Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato
Hydrogen sulfide (H(2)S), a novel gasotransmitter in both mammals and plants, plays important roles in plant development and stress responses. Leaf senescence represents the final stage of leaf development. The role of H(2)S-producing enzyme L-cysteine desulfhydrase in regulating tomato leaf senesce...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658025/ https://www.ncbi.nlm.nih.gov/pubmed/34884883 http://dx.doi.org/10.3390/ijms222313078 |
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author | Hu, Kangdi Peng, Xiangjun Yao, Gaifang Zhou, Zhilin Yang, Feng Li, Wanjie Zhao, Yuqi Li, Yanhong Han, Zhuo Chen, Xiaoyan Zhang, Hua |
author_facet | Hu, Kangdi Peng, Xiangjun Yao, Gaifang Zhou, Zhilin Yang, Feng Li, Wanjie Zhao, Yuqi Li, Yanhong Han, Zhuo Chen, Xiaoyan Zhang, Hua |
author_sort | Hu, Kangdi |
collection | PubMed |
description | Hydrogen sulfide (H(2)S), a novel gasotransmitter in both mammals and plants, plays important roles in plant development and stress responses. Leaf senescence represents the final stage of leaf development. The role of H(2)S-producing enzyme L-cysteine desulfhydrase in regulating tomato leaf senescence is still unknown. In the present study, the effect of an L-cysteine desulfhydrase LCD1 on leaf senescence in tomato was explored by physiological analysis. LCD1 mutation caused earlier leaf senescence, whereas LCD1 overexpression significantly delayed leaf senescence compared with the wild type in 10-week tomato seedlings. Moreover, LCD1 overexpression was found to delay dark-induced senescence in detached tomato leaves, and the lcd1 mutant showed accelerated senescence. An increasing trend of H(2)S production was observed in leaves during storage in darkness, while LCD1 deletion reduced H(2)S production and LCD1 overexpression produced more H(2)S compared with the wild-type control. Further investigations showed that LCD1 overexpression delayed dark-triggered chlorophyll degradation and reactive oxygen species (ROS) accumulation in detached tomato leaves, and the increase in the expression of chlorophyll degradation genes NYC1, PAO, PPH, SGR1, and senescence-associated genes (SAGs) during senescence was attenuated by LCD1 overexpression, whereas lcd1 mutants showed enhanced senescence-related parameters. Moreover, a correlation analysis indicated that chlorophyll content was negatively correlated with H(2)O(2) and malondialdehyde (MDA) content, and also negatively correlated with the expression of chlorophyll degradation-related genes and SAGs. Therefore, these findings increase our understanding of the physiological functions of the H(2)S-generating enzyme LCD1 in regulating leaf senescence in tomato. |
format | Online Article Text |
id | pubmed-8658025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86580252021-12-10 Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato Hu, Kangdi Peng, Xiangjun Yao, Gaifang Zhou, Zhilin Yang, Feng Li, Wanjie Zhao, Yuqi Li, Yanhong Han, Zhuo Chen, Xiaoyan Zhang, Hua Int J Mol Sci Article Hydrogen sulfide (H(2)S), a novel gasotransmitter in both mammals and plants, plays important roles in plant development and stress responses. Leaf senescence represents the final stage of leaf development. The role of H(2)S-producing enzyme L-cysteine desulfhydrase in regulating tomato leaf senescence is still unknown. In the present study, the effect of an L-cysteine desulfhydrase LCD1 on leaf senescence in tomato was explored by physiological analysis. LCD1 mutation caused earlier leaf senescence, whereas LCD1 overexpression significantly delayed leaf senescence compared with the wild type in 10-week tomato seedlings. Moreover, LCD1 overexpression was found to delay dark-induced senescence in detached tomato leaves, and the lcd1 mutant showed accelerated senescence. An increasing trend of H(2)S production was observed in leaves during storage in darkness, while LCD1 deletion reduced H(2)S production and LCD1 overexpression produced more H(2)S compared with the wild-type control. Further investigations showed that LCD1 overexpression delayed dark-triggered chlorophyll degradation and reactive oxygen species (ROS) accumulation in detached tomato leaves, and the increase in the expression of chlorophyll degradation genes NYC1, PAO, PPH, SGR1, and senescence-associated genes (SAGs) during senescence was attenuated by LCD1 overexpression, whereas lcd1 mutants showed enhanced senescence-related parameters. Moreover, a correlation analysis indicated that chlorophyll content was negatively correlated with H(2)O(2) and malondialdehyde (MDA) content, and also negatively correlated with the expression of chlorophyll degradation-related genes and SAGs. Therefore, these findings increase our understanding of the physiological functions of the H(2)S-generating enzyme LCD1 in regulating leaf senescence in tomato. MDPI 2021-12-03 /pmc/articles/PMC8658025/ /pubmed/34884883 http://dx.doi.org/10.3390/ijms222313078 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hu, Kangdi Peng, Xiangjun Yao, Gaifang Zhou, Zhilin Yang, Feng Li, Wanjie Zhao, Yuqi Li, Yanhong Han, Zhuo Chen, Xiaoyan Zhang, Hua Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato |
title | Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato |
title_full | Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato |
title_fullStr | Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato |
title_full_unstemmed | Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato |
title_short | Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato |
title_sort | roles of a cysteine desulfhydrase lcd1 in regulating leaf senescence in tomato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658025/ https://www.ncbi.nlm.nih.gov/pubmed/34884883 http://dx.doi.org/10.3390/ijms222313078 |
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