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Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro

Neochlorogenic acid (5-Caffeoylquinic acid; 5-CQA), a major phenolic compound isolated from mulberry leaves, possesses anti-oxidative and anti-inflammatory effects. Although it modulates lipid metabolism, the molecular mechanism is unknown. Using an in-vitro model of nonalcoholic fatty liver disease...

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Autores principales: Yu, Meng-Hsun, Hung, Tung-Wei, Wang, Chi-Chih, Wu, Sheng-Wen, Yang, Tzu-Wei, Yang, Ching-Yu, Tseng, Tsui-Hwa, Wang, Chau-Jong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658127/
https://www.ncbi.nlm.nih.gov/pubmed/34884968
http://dx.doi.org/10.3390/ijms222313163
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author Yu, Meng-Hsun
Hung, Tung-Wei
Wang, Chi-Chih
Wu, Sheng-Wen
Yang, Tzu-Wei
Yang, Ching-Yu
Tseng, Tsui-Hwa
Wang, Chau-Jong
author_facet Yu, Meng-Hsun
Hung, Tung-Wei
Wang, Chi-Chih
Wu, Sheng-Wen
Yang, Tzu-Wei
Yang, Ching-Yu
Tseng, Tsui-Hwa
Wang, Chau-Jong
author_sort Yu, Meng-Hsun
collection PubMed
description Neochlorogenic acid (5-Caffeoylquinic acid; 5-CQA), a major phenolic compound isolated from mulberry leaves, possesses anti-oxidative and anti-inflammatory effects. Although it modulates lipid metabolism, the molecular mechanism is unknown. Using an in-vitro model of nonalcoholic fatty liver disease (NAFLD) in which oleic acid (OA) induced lipid accumulation in HepG2 cells, we evaluated the alleviation effect of 5-CQA. We observed that 5-CQA improved OA-induced intracellular lipid accumulation by downregulating sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FASN) expression, which regulates the fatty acid synthesis, as well as SREBP2 and HMG-CoA reductases (HMG-CoR) expressions, which regulate cholesterol synthesis. Treatment with 5-CQA also increased the expression of fatty acid β-oxidation enzymes. Remarkably, 5-CQA attenuated OA-induced miR-34a expression. A transfection assay with an miR-34a mimic or miR-34a inhibitor revealed that miR-34a suppressed Moreover, Sirtuin 1 (SIRT1) expression and inactivated 5’ adenosine monophosphate-activated protein kinase (AMPK). Our results suggest that 5-CQA alleviates lipid accumulation by downregulating miR-34a, leading to activation of the SIRT1/AMPK pathway.
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spelling pubmed-86581272021-12-10 Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro Yu, Meng-Hsun Hung, Tung-Wei Wang, Chi-Chih Wu, Sheng-Wen Yang, Tzu-Wei Yang, Ching-Yu Tseng, Tsui-Hwa Wang, Chau-Jong Int J Mol Sci Article Neochlorogenic acid (5-Caffeoylquinic acid; 5-CQA), a major phenolic compound isolated from mulberry leaves, possesses anti-oxidative and anti-inflammatory effects. Although it modulates lipid metabolism, the molecular mechanism is unknown. Using an in-vitro model of nonalcoholic fatty liver disease (NAFLD) in which oleic acid (OA) induced lipid accumulation in HepG2 cells, we evaluated the alleviation effect of 5-CQA. We observed that 5-CQA improved OA-induced intracellular lipid accumulation by downregulating sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FASN) expression, which regulates the fatty acid synthesis, as well as SREBP2 and HMG-CoA reductases (HMG-CoR) expressions, which regulate cholesterol synthesis. Treatment with 5-CQA also increased the expression of fatty acid β-oxidation enzymes. Remarkably, 5-CQA attenuated OA-induced miR-34a expression. A transfection assay with an miR-34a mimic or miR-34a inhibitor revealed that miR-34a suppressed Moreover, Sirtuin 1 (SIRT1) expression and inactivated 5’ adenosine monophosphate-activated protein kinase (AMPK). Our results suggest that 5-CQA alleviates lipid accumulation by downregulating miR-34a, leading to activation of the SIRT1/AMPK pathway. MDPI 2021-12-06 /pmc/articles/PMC8658127/ /pubmed/34884968 http://dx.doi.org/10.3390/ijms222313163 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Meng-Hsun
Hung, Tung-Wei
Wang, Chi-Chih
Wu, Sheng-Wen
Yang, Tzu-Wei
Yang, Ching-Yu
Tseng, Tsui-Hwa
Wang, Chau-Jong
Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro
title Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro
title_full Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro
title_fullStr Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro
title_full_unstemmed Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro
title_short Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro
title_sort neochlorogenic acid attenuates hepatic lipid accumulation and inflammation via regulating mir-34a in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658127/
https://www.ncbi.nlm.nih.gov/pubmed/34884968
http://dx.doi.org/10.3390/ijms222313163
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