Cargando…
Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry
Osteoarthritis is a common multifactorial chronic disease that occurs in articular cartilage, subchondral bone, and periarticular tissue. The pathogenesis of OA is still unclear. To investigate the differences in serum metabolites between OA and the control group, liquid chromatography/mass spectrom...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658788/ https://www.ncbi.nlm.nih.gov/pubmed/34885759 http://dx.doi.org/10.3390/molecules26237181 |
_version_ | 1784612810202808320 |
---|---|
author | Zhao, Jingtong Liu, Meng Shi, Tongfei Gao, Mohan Lv, Yuqian Zhao, Yawei Li, Jing Zhang, Ming Zhang, Hansi Guan, Fengying He, Kan Chen, Li |
author_facet | Zhao, Jingtong Liu, Meng Shi, Tongfei Gao, Mohan Lv, Yuqian Zhao, Yawei Li, Jing Zhang, Ming Zhang, Hansi Guan, Fengying He, Kan Chen, Li |
author_sort | Zhao, Jingtong |
collection | PubMed |
description | Osteoarthritis is a common multifactorial chronic disease that occurs in articular cartilage, subchondral bone, and periarticular tissue. The pathogenesis of OA is still unclear. To investigate the differences in serum metabolites between OA and the control group, liquid chromatography/mass spectrometry (LC/MS)-based metabolomics was used. To reveal the pathogenesis of OA, 12 SD male rats were randomly divided into control and OA groups using collagenase to induce OA for modeling, and serum was collected 7 days after modeling for testing. The OA group was distinguished from the control group by principal component analysis and orthogonal partial least squares-discriminant analysis, and six biomarkers were finally identified. These biomarkers were metabolized through tryptophan metabolism, glutamate metabolism, nitrogen metabolism, spermidine metabolism, and fatty acid metabolism pathways. The study identified metabolites that may be altered in OA, suggesting a role in OA through relevant metabolic pathways. Metabolomics, as an important tool for studying disease mechanisms, provides useful information for studying the metabolic mechanisms of OA. |
format | Online Article Text |
id | pubmed-8658788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86587882021-12-10 Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry Zhao, Jingtong Liu, Meng Shi, Tongfei Gao, Mohan Lv, Yuqian Zhao, Yawei Li, Jing Zhang, Ming Zhang, Hansi Guan, Fengying He, Kan Chen, Li Molecules Article Osteoarthritis is a common multifactorial chronic disease that occurs in articular cartilage, subchondral bone, and periarticular tissue. The pathogenesis of OA is still unclear. To investigate the differences in serum metabolites between OA and the control group, liquid chromatography/mass spectrometry (LC/MS)-based metabolomics was used. To reveal the pathogenesis of OA, 12 SD male rats were randomly divided into control and OA groups using collagenase to induce OA for modeling, and serum was collected 7 days after modeling for testing. The OA group was distinguished from the control group by principal component analysis and orthogonal partial least squares-discriminant analysis, and six biomarkers were finally identified. These biomarkers were metabolized through tryptophan metabolism, glutamate metabolism, nitrogen metabolism, spermidine metabolism, and fatty acid metabolism pathways. The study identified metabolites that may be altered in OA, suggesting a role in OA through relevant metabolic pathways. Metabolomics, as an important tool for studying disease mechanisms, provides useful information for studying the metabolic mechanisms of OA. MDPI 2021-11-26 /pmc/articles/PMC8658788/ /pubmed/34885759 http://dx.doi.org/10.3390/molecules26237181 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Jingtong Liu, Meng Shi, Tongfei Gao, Mohan Lv, Yuqian Zhao, Yawei Li, Jing Zhang, Ming Zhang, Hansi Guan, Fengying He, Kan Chen, Li Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry |
title | Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry |
title_full | Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry |
title_fullStr | Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry |
title_full_unstemmed | Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry |
title_short | Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry |
title_sort | analysis of serum metabolomics in rats with osteoarthritis by mass spectrometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658788/ https://www.ncbi.nlm.nih.gov/pubmed/34885759 http://dx.doi.org/10.3390/molecules26237181 |
work_keys_str_mv | AT zhaojingtong analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT liumeng analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT shitongfei analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT gaomohan analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT lvyuqian analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT zhaoyawei analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT lijing analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT zhangming analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT zhanghansi analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT guanfengying analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT hekan analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry AT chenli analysisofserummetabolomicsinratswithosteoarthritisbymassspectrometry |