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Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry

Osteoarthritis is a common multifactorial chronic disease that occurs in articular cartilage, subchondral bone, and periarticular tissue. The pathogenesis of OA is still unclear. To investigate the differences in serum metabolites between OA and the control group, liquid chromatography/mass spectrom...

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Autores principales: Zhao, Jingtong, Liu, Meng, Shi, Tongfei, Gao, Mohan, Lv, Yuqian, Zhao, Yawei, Li, Jing, Zhang, Ming, Zhang, Hansi, Guan, Fengying, He, Kan, Chen, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658788/
https://www.ncbi.nlm.nih.gov/pubmed/34885759
http://dx.doi.org/10.3390/molecules26237181
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author Zhao, Jingtong
Liu, Meng
Shi, Tongfei
Gao, Mohan
Lv, Yuqian
Zhao, Yawei
Li, Jing
Zhang, Ming
Zhang, Hansi
Guan, Fengying
He, Kan
Chen, Li
author_facet Zhao, Jingtong
Liu, Meng
Shi, Tongfei
Gao, Mohan
Lv, Yuqian
Zhao, Yawei
Li, Jing
Zhang, Ming
Zhang, Hansi
Guan, Fengying
He, Kan
Chen, Li
author_sort Zhao, Jingtong
collection PubMed
description Osteoarthritis is a common multifactorial chronic disease that occurs in articular cartilage, subchondral bone, and periarticular tissue. The pathogenesis of OA is still unclear. To investigate the differences in serum metabolites between OA and the control group, liquid chromatography/mass spectrometry (LC/MS)-based metabolomics was used. To reveal the pathogenesis of OA, 12 SD male rats were randomly divided into control and OA groups using collagenase to induce OA for modeling, and serum was collected 7 days after modeling for testing. The OA group was distinguished from the control group by principal component analysis and orthogonal partial least squares-discriminant analysis, and six biomarkers were finally identified. These biomarkers were metabolized through tryptophan metabolism, glutamate metabolism, nitrogen metabolism, spermidine metabolism, and fatty acid metabolism pathways. The study identified metabolites that may be altered in OA, suggesting a role in OA through relevant metabolic pathways. Metabolomics, as an important tool for studying disease mechanisms, provides useful information for studying the metabolic mechanisms of OA.
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spelling pubmed-86587882021-12-10 Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry Zhao, Jingtong Liu, Meng Shi, Tongfei Gao, Mohan Lv, Yuqian Zhao, Yawei Li, Jing Zhang, Ming Zhang, Hansi Guan, Fengying He, Kan Chen, Li Molecules Article Osteoarthritis is a common multifactorial chronic disease that occurs in articular cartilage, subchondral bone, and periarticular tissue. The pathogenesis of OA is still unclear. To investigate the differences in serum metabolites between OA and the control group, liquid chromatography/mass spectrometry (LC/MS)-based metabolomics was used. To reveal the pathogenesis of OA, 12 SD male rats were randomly divided into control and OA groups using collagenase to induce OA for modeling, and serum was collected 7 days after modeling for testing. The OA group was distinguished from the control group by principal component analysis and orthogonal partial least squares-discriminant analysis, and six biomarkers were finally identified. These biomarkers were metabolized through tryptophan metabolism, glutamate metabolism, nitrogen metabolism, spermidine metabolism, and fatty acid metabolism pathways. The study identified metabolites that may be altered in OA, suggesting a role in OA through relevant metabolic pathways. Metabolomics, as an important tool for studying disease mechanisms, provides useful information for studying the metabolic mechanisms of OA. MDPI 2021-11-26 /pmc/articles/PMC8658788/ /pubmed/34885759 http://dx.doi.org/10.3390/molecules26237181 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Jingtong
Liu, Meng
Shi, Tongfei
Gao, Mohan
Lv, Yuqian
Zhao, Yawei
Li, Jing
Zhang, Ming
Zhang, Hansi
Guan, Fengying
He, Kan
Chen, Li
Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry
title Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry
title_full Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry
title_fullStr Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry
title_full_unstemmed Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry
title_short Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry
title_sort analysis of serum metabolomics in rats with osteoarthritis by mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658788/
https://www.ncbi.nlm.nih.gov/pubmed/34885759
http://dx.doi.org/10.3390/molecules26237181
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