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Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS

In recent years, capillary electrophoresis coupled to mass spectrometry (CE-MS) has been increasingly applied in clinical research especially in the context of chronic and age-associated diseases, such as chronic kidney disease, heart failure and cancer. Biomarkers identified using this technique ar...

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Autores principales: Mavrogeorgis, Emmanouil, Mischak, Harald, Latosinska, Agnieszka, Siwy, Justyna, Jankowski, Vera, Jankowski, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658976/
https://www.ncbi.nlm.nih.gov/pubmed/34885840
http://dx.doi.org/10.3390/molecules26237260
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author Mavrogeorgis, Emmanouil
Mischak, Harald
Latosinska, Agnieszka
Siwy, Justyna
Jankowski, Vera
Jankowski, Joachim
author_facet Mavrogeorgis, Emmanouil
Mischak, Harald
Latosinska, Agnieszka
Siwy, Justyna
Jankowski, Vera
Jankowski, Joachim
author_sort Mavrogeorgis, Emmanouil
collection PubMed
description In recent years, capillary electrophoresis coupled to mass spectrometry (CE-MS) has been increasingly applied in clinical research especially in the context of chronic and age-associated diseases, such as chronic kidney disease, heart failure and cancer. Biomarkers identified using this technique are already used for diagnosis, prognosis and monitoring of these complex diseases, as well as patient stratification in clinical trials. CE-MS allows for a comprehensive assessment of small molecular weight proteins and peptides (<20 kDa) through the combination of the high resolution and reproducibility of CE and the distinct sensitivity of MS, in a high-throughput system. In this study we assessed CE-MS analytical performance with regards to its inter- and intra-day reproducibility, variability and efficiency in peptide detection, along with a characterization of the urinary peptidome content. To this end, CE-MS performance was evaluated based on 72 measurements of a standard urine sample (60 for inter- and 12 for intra-day assessment) analyzed during the second quarter of 2021. Analysis was performed per run, per peptide, as well as at the level of biomarker panels. The obtained datasets showed high correlation between the different runs, low variation of the ten highest average individual log2 signal intensities (coefficient of variation, CV < 10%) and very low variation of biomarker panels applied (CV close to 1%). The findings of the study support the analytical performance of CE-MS, underlining its value for clinical application.
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spelling pubmed-86589762021-12-10 Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS Mavrogeorgis, Emmanouil Mischak, Harald Latosinska, Agnieszka Siwy, Justyna Jankowski, Vera Jankowski, Joachim Molecules Article In recent years, capillary electrophoresis coupled to mass spectrometry (CE-MS) has been increasingly applied in clinical research especially in the context of chronic and age-associated diseases, such as chronic kidney disease, heart failure and cancer. Biomarkers identified using this technique are already used for diagnosis, prognosis and monitoring of these complex diseases, as well as patient stratification in clinical trials. CE-MS allows for a comprehensive assessment of small molecular weight proteins and peptides (<20 kDa) through the combination of the high resolution and reproducibility of CE and the distinct sensitivity of MS, in a high-throughput system. In this study we assessed CE-MS analytical performance with regards to its inter- and intra-day reproducibility, variability and efficiency in peptide detection, along with a characterization of the urinary peptidome content. To this end, CE-MS performance was evaluated based on 72 measurements of a standard urine sample (60 for inter- and 12 for intra-day assessment) analyzed during the second quarter of 2021. Analysis was performed per run, per peptide, as well as at the level of biomarker panels. The obtained datasets showed high correlation between the different runs, low variation of the ten highest average individual log2 signal intensities (coefficient of variation, CV < 10%) and very low variation of biomarker panels applied (CV close to 1%). The findings of the study support the analytical performance of CE-MS, underlining its value for clinical application. MDPI 2021-11-30 /pmc/articles/PMC8658976/ /pubmed/34885840 http://dx.doi.org/10.3390/molecules26237260 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mavrogeorgis, Emmanouil
Mischak, Harald
Latosinska, Agnieszka
Siwy, Justyna
Jankowski, Vera
Jankowski, Joachim
Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS
title Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS
title_full Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS
title_fullStr Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS
title_full_unstemmed Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS
title_short Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS
title_sort reproducibility evaluation of urinary peptide detection using ce-ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658976/
https://www.ncbi.nlm.nih.gov/pubmed/34885840
http://dx.doi.org/10.3390/molecules26237260
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