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Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies

Zn(II) complexes bearing tris[3-(2-pyridyl)-pyrazolyl] borate (Tp(py)) ligand (1–3) was synthesized and examined by spectroscopic and analytical tools. Mononuclear [Tp(py)ZnCl] (1) has a Zn(II) centre with one arm (pyrazolyl-pyridyl) dangling outside the coordination sphere which is a novel finding...

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Autores principales: Narwane, Manmath, Dorairaj, Dorothy Priyanka, Chang, Yu-Lun, Karvembu, Ramasamy, Huang, Yu-Han, Chang, Hsueh-Wei, Hsu, Sodio C. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659194/
https://www.ncbi.nlm.nih.gov/pubmed/34885924
http://dx.doi.org/10.3390/molecules26237341
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author Narwane, Manmath
Dorairaj, Dorothy Priyanka
Chang, Yu-Lun
Karvembu, Ramasamy
Huang, Yu-Han
Chang, Hsueh-Wei
Hsu, Sodio C. N.
author_facet Narwane, Manmath
Dorairaj, Dorothy Priyanka
Chang, Yu-Lun
Karvembu, Ramasamy
Huang, Yu-Han
Chang, Hsueh-Wei
Hsu, Sodio C. N.
author_sort Narwane, Manmath
collection PubMed
description Zn(II) complexes bearing tris[3-(2-pyridyl)-pyrazolyl] borate (Tp(py)) ligand (1–3) was synthesized and examined by spectroscopic and analytical tools. Mononuclear [Tp(py)ZnCl] (1) has a Zn(II) centre with one arm (pyrazolyl-pyridyl) dangling outside the coordination sphere which is a novel finding in Tp(py)Zn(II) chemistry. In complex [Tp(py)Zn(H(2)O)][BF(4)] (2) hydrogen bonding interaction of aqua moiety stabilizes the dangling arm. In addition, solution state behaviour of complex 1 confirms the tridentate binding mode and reactivity studies show the exogenous axial substituents used to form the [Tp(py)ZnN(3)] (3). The complexes (1–3) were tested for their ability to bind with Calf thymus (CT) DNA and Bovine serum albumin (BSA) wherein they revealed to exhibit good binding constant values with both the biomolecules in the order of 10(4)–10(5) M(−1). The intercalative binding mode with CT DNA was confirmed from the UV-Visible absorption, viscosity, and ethidium bromide (EB) DNA displacement studies. Further, the complexes were tested for in vitro cytotoxic ability on four triple-negative breast cancer (TNBC) cell lines (MDA-MB-231, MDA-MB-468, HCC1937, and Hs 578T). All three complexes (1–3) exhibited good IC(50) values (6.81 to 16.87 μM for 24 h as seen from the MTS assay) results which indicated that these complexes were found to be potential anticancer agents against the TNBC cells.
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spelling pubmed-86591942021-12-10 Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies Narwane, Manmath Dorairaj, Dorothy Priyanka Chang, Yu-Lun Karvembu, Ramasamy Huang, Yu-Han Chang, Hsueh-Wei Hsu, Sodio C. N. Molecules Article Zn(II) complexes bearing tris[3-(2-pyridyl)-pyrazolyl] borate (Tp(py)) ligand (1–3) was synthesized and examined by spectroscopic and analytical tools. Mononuclear [Tp(py)ZnCl] (1) has a Zn(II) centre with one arm (pyrazolyl-pyridyl) dangling outside the coordination sphere which is a novel finding in Tp(py)Zn(II) chemistry. In complex [Tp(py)Zn(H(2)O)][BF(4)] (2) hydrogen bonding interaction of aqua moiety stabilizes the dangling arm. In addition, solution state behaviour of complex 1 confirms the tridentate binding mode and reactivity studies show the exogenous axial substituents used to form the [Tp(py)ZnN(3)] (3). The complexes (1–3) were tested for their ability to bind with Calf thymus (CT) DNA and Bovine serum albumin (BSA) wherein they revealed to exhibit good binding constant values with both the biomolecules in the order of 10(4)–10(5) M(−1). The intercalative binding mode with CT DNA was confirmed from the UV-Visible absorption, viscosity, and ethidium bromide (EB) DNA displacement studies. Further, the complexes were tested for in vitro cytotoxic ability on four triple-negative breast cancer (TNBC) cell lines (MDA-MB-231, MDA-MB-468, HCC1937, and Hs 578T). All three complexes (1–3) exhibited good IC(50) values (6.81 to 16.87 μM for 24 h as seen from the MTS assay) results which indicated that these complexes were found to be potential anticancer agents against the TNBC cells. MDPI 2021-12-03 /pmc/articles/PMC8659194/ /pubmed/34885924 http://dx.doi.org/10.3390/molecules26237341 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Narwane, Manmath
Dorairaj, Dorothy Priyanka
Chang, Yu-Lun
Karvembu, Ramasamy
Huang, Yu-Han
Chang, Hsueh-Wei
Hsu, Sodio C. N.
Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies
title Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies
title_full Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies
title_fullStr Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies
title_full_unstemmed Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies
title_short Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies
title_sort tris-(2-pyridyl)-pyrazolyl borate zinc(ii) complexes: synthesis, dna/protein binding and in vitro cytotoxicity studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659194/
https://www.ncbi.nlm.nih.gov/pubmed/34885924
http://dx.doi.org/10.3390/molecules26237341
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