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Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off

Dairy cows at dry-off undergo several management and physiological changes, resulting in alterations in plasma biomarkers of inflammation, oxidative stress, and immune system. High milk yield at the end of lactation exacerbates these responses. The underlying mechanism of these changes has yet to be...

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Autores principales: Cattaneo, Luca, Mezzetti, Matteo, Lopreiato, Vincenzo, Piccioli-Cappelli, Fiorenzo, Trevisi, Erminio, Minuti, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659302/
https://www.ncbi.nlm.nih.gov/pubmed/34882732
http://dx.doi.org/10.1371/journal.pone.0260745
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author Cattaneo, Luca
Mezzetti, Matteo
Lopreiato, Vincenzo
Piccioli-Cappelli, Fiorenzo
Trevisi, Erminio
Minuti, Andrea
author_facet Cattaneo, Luca
Mezzetti, Matteo
Lopreiato, Vincenzo
Piccioli-Cappelli, Fiorenzo
Trevisi, Erminio
Minuti, Andrea
author_sort Cattaneo, Luca
collection PubMed
description Dairy cows at dry-off undergo several management and physiological changes, resulting in alterations in plasma biomarkers of inflammation, oxidative stress, and immune system. High milk yield at the end of lactation exacerbates these responses. The underlying mechanism of these changes has yet to be elucidated. We hypothesized altered leukocyte gene expression after dry-off and different responses in cows with different milk yield. Thirteen Holstein dairy cows were sampled at the turn of dry-off to investigated whole blood leukocyte gene expression and were grouped according to the average milk yield during the last week of lactation: low (< 15 kg/d) and high milk yield (> 15 kg/d). Blood samples were collected in PAXgene tubes (Preanalytix, Hombrechtikon, Switzerland) at -7, 7, and 34 days from dry-off (DFD) to measure mRNA abundance of 37 genes. Normalized gene abundance data were subjected to MIXED model ANOVA (SAS Institute Inc., Cary, NC). Compared with -7 DFD, at 7 DFD RNA abundance of lipoxygenase genes (ALOX5, ALOX15) and myeloperoxidase (MPO) increased, and that of the antioxidant gene (SOD2) decreased. Meanwhile, genes related to recognition and immune mediation (CD16, MYD88, TLR2), migration and cell adhesion (CX3CR1, ITGAL, ITGB2, TLN1), and the antimicrobial gene MMP9 were downregulated at 7 or 34 DFD, whereas the antimicrobial IDO1 gene was upregulated. Compared with low-producing cows, cows with high milk yield at dry-off cows had upregulated expression of the pro-inflammatory cytokines IL8 and IL18 and a greater reduction in transcript abundance of the toll-like receptor (TLR) recognition-related gene TLR2. Overall, the dry-off confirmed to be a phase of intense changes, triggering an inflammatory response and somewhat suppressing leukocyte immune function. In cows with high milk yield during the week before dry-off, the inflammatory response was exacerbated.
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spelling pubmed-86593022021-12-10 Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off Cattaneo, Luca Mezzetti, Matteo Lopreiato, Vincenzo Piccioli-Cappelli, Fiorenzo Trevisi, Erminio Minuti, Andrea PLoS One Research Article Dairy cows at dry-off undergo several management and physiological changes, resulting in alterations in plasma biomarkers of inflammation, oxidative stress, and immune system. High milk yield at the end of lactation exacerbates these responses. The underlying mechanism of these changes has yet to be elucidated. We hypothesized altered leukocyte gene expression after dry-off and different responses in cows with different milk yield. Thirteen Holstein dairy cows were sampled at the turn of dry-off to investigated whole blood leukocyte gene expression and were grouped according to the average milk yield during the last week of lactation: low (< 15 kg/d) and high milk yield (> 15 kg/d). Blood samples were collected in PAXgene tubes (Preanalytix, Hombrechtikon, Switzerland) at -7, 7, and 34 days from dry-off (DFD) to measure mRNA abundance of 37 genes. Normalized gene abundance data were subjected to MIXED model ANOVA (SAS Institute Inc., Cary, NC). Compared with -7 DFD, at 7 DFD RNA abundance of lipoxygenase genes (ALOX5, ALOX15) and myeloperoxidase (MPO) increased, and that of the antioxidant gene (SOD2) decreased. Meanwhile, genes related to recognition and immune mediation (CD16, MYD88, TLR2), migration and cell adhesion (CX3CR1, ITGAL, ITGB2, TLN1), and the antimicrobial gene MMP9 were downregulated at 7 or 34 DFD, whereas the antimicrobial IDO1 gene was upregulated. Compared with low-producing cows, cows with high milk yield at dry-off cows had upregulated expression of the pro-inflammatory cytokines IL8 and IL18 and a greater reduction in transcript abundance of the toll-like receptor (TLR) recognition-related gene TLR2. Overall, the dry-off confirmed to be a phase of intense changes, triggering an inflammatory response and somewhat suppressing leukocyte immune function. In cows with high milk yield during the week before dry-off, the inflammatory response was exacerbated. Public Library of Science 2021-12-09 /pmc/articles/PMC8659302/ /pubmed/34882732 http://dx.doi.org/10.1371/journal.pone.0260745 Text en © 2021 Cattaneo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cattaneo, Luca
Mezzetti, Matteo
Lopreiato, Vincenzo
Piccioli-Cappelli, Fiorenzo
Trevisi, Erminio
Minuti, Andrea
Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off
title Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off
title_full Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off
title_fullStr Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off
title_full_unstemmed Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off
title_short Gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off
title_sort gene network expression of whole blood leukocytes in dairy cows with different milk yield at dry-off
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659302/
https://www.ncbi.nlm.nih.gov/pubmed/34882732
http://dx.doi.org/10.1371/journal.pone.0260745
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