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Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation
Acupuncture treatment is based on acupoint stimulation; however, the biological basis is not understood. We stimulated one acupoint with catgut embedding for 8 weeks and then used isobaric tags for relative and absolute quantitation to screen proteins with altered expression in adjacent acupoints of...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659396/ https://www.ncbi.nlm.nih.gov/pubmed/34909734 http://dx.doi.org/10.1016/j.xjidi.2021.100038 |
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author | Wang, Ting Zhu, Geng Qin, Liyue Wang, Qian She, Chen Xu, Dongsheng Hu, Weiwei Luo, Kenghuo Lei, Ying Gong, Yanling Ghosh, Arijit Ma, Dongni Ding, Chun-Lei Wang, Bu-Yi Guo, Yang Ma, Shou-Shan Hattori, Michihiro Takagi, Yutaka Ara, Katsutoshi Higuchi, Kazuhiko Li, Xingwang He, Lin Bai, Wanzhu Ishida, Koichi Li, Sheng-Tian |
author_facet | Wang, Ting Zhu, Geng Qin, Liyue Wang, Qian She, Chen Xu, Dongsheng Hu, Weiwei Luo, Kenghuo Lei, Ying Gong, Yanling Ghosh, Arijit Ma, Dongni Ding, Chun-Lei Wang, Bu-Yi Guo, Yang Ma, Shou-Shan Hattori, Michihiro Takagi, Yutaka Ara, Katsutoshi Higuchi, Kazuhiko Li, Xingwang He, Lin Bai, Wanzhu Ishida, Koichi Li, Sheng-Tian |
author_sort | Wang, Ting |
collection | PubMed |
description | Acupuncture treatment is based on acupoint stimulation; however, the biological basis is not understood. We stimulated one acupoint with catgut embedding for 8 weeks and then used isobaric tags for relative and absolute quantitation to screen proteins with altered expression in adjacent acupoints of Sprague Dawley rats. We found that kininogen expression was significantly upregulated in the stimulated and the nonstimulated adjacent acupoints along the same meridian. The enhanced kininogen expression was meridian dependent and was most apparent among small vessels in the subcutaneous layer. Enhanced signals of nitric oxide synthases, cGMP-dependent protein kinase, and myosin light chain were also observed at the nonstimulated adjacent acupoints along the same meridian. These findings uncover biological changes at acupoints and suggest the critical role of the kininogen–nitric oxide signaling pathway in acupoint activation. |
format | Online Article Text |
id | pubmed-8659396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-86593962021-12-13 Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation Wang, Ting Zhu, Geng Qin, Liyue Wang, Qian She, Chen Xu, Dongsheng Hu, Weiwei Luo, Kenghuo Lei, Ying Gong, Yanling Ghosh, Arijit Ma, Dongni Ding, Chun-Lei Wang, Bu-Yi Guo, Yang Ma, Shou-Shan Hattori, Michihiro Takagi, Yutaka Ara, Katsutoshi Higuchi, Kazuhiko Li, Xingwang He, Lin Bai, Wanzhu Ishida, Koichi Li, Sheng-Tian JID Innov Original Article Acupuncture treatment is based on acupoint stimulation; however, the biological basis is not understood. We stimulated one acupoint with catgut embedding for 8 weeks and then used isobaric tags for relative and absolute quantitation to screen proteins with altered expression in adjacent acupoints of Sprague Dawley rats. We found that kininogen expression was significantly upregulated in the stimulated and the nonstimulated adjacent acupoints along the same meridian. The enhanced kininogen expression was meridian dependent and was most apparent among small vessels in the subcutaneous layer. Enhanced signals of nitric oxide synthases, cGMP-dependent protein kinase, and myosin light chain were also observed at the nonstimulated adjacent acupoints along the same meridian. These findings uncover biological changes at acupoints and suggest the critical role of the kininogen–nitric oxide signaling pathway in acupoint activation. Elsevier 2021-06-24 /pmc/articles/PMC8659396/ /pubmed/34909734 http://dx.doi.org/10.1016/j.xjidi.2021.100038 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Wang, Ting Zhu, Geng Qin, Liyue Wang, Qian She, Chen Xu, Dongsheng Hu, Weiwei Luo, Kenghuo Lei, Ying Gong, Yanling Ghosh, Arijit Ma, Dongni Ding, Chun-Lei Wang, Bu-Yi Guo, Yang Ma, Shou-Shan Hattori, Michihiro Takagi, Yutaka Ara, Katsutoshi Higuchi, Kazuhiko Li, Xingwang He, Lin Bai, Wanzhu Ishida, Koichi Li, Sheng-Tian Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation |
title | Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation |
title_full | Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation |
title_fullStr | Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation |
title_full_unstemmed | Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation |
title_short | Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation |
title_sort | kininogen–nitric oxide signaling at nearby nonexcited acupoints after long-term stimulation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659396/ https://www.ncbi.nlm.nih.gov/pubmed/34909734 http://dx.doi.org/10.1016/j.xjidi.2021.100038 |
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