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Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose

One of the ways to enhance the yield of bacterial cellulose (BC) is by using dynamic aeration and different-type bioreactors because the microbial producers are strict aerobes. But in this case, the BC quality tends to worsen. Here we have combined static culture with aeration in the biosynthesis of...

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Autores principales: Shavyrkina, Nadezhda A., Skiba, Ekaterina A., Kazantseva, Anastasia E., Gladysheva, Evgenia K., Budaeva, Vera V., Bychin, Nikolay V., Gismatulina, Yulia A., Kashcheyeva, Ekaterina I., Mironova, Galina F., Korchagina, Anna A., Pavlov, Igor N., Sakovich, Gennady V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659626/
https://www.ncbi.nlm.nih.gov/pubmed/34883747
http://dx.doi.org/10.3390/polym13234241
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author Shavyrkina, Nadezhda A.
Skiba, Ekaterina A.
Kazantseva, Anastasia E.
Gladysheva, Evgenia K.
Budaeva, Vera V.
Bychin, Nikolay V.
Gismatulina, Yulia A.
Kashcheyeva, Ekaterina I.
Mironova, Galina F.
Korchagina, Anna A.
Pavlov, Igor N.
Sakovich, Gennady V.
author_facet Shavyrkina, Nadezhda A.
Skiba, Ekaterina A.
Kazantseva, Anastasia E.
Gladysheva, Evgenia K.
Budaeva, Vera V.
Bychin, Nikolay V.
Gismatulina, Yulia A.
Kashcheyeva, Ekaterina I.
Mironova, Galina F.
Korchagina, Anna A.
Pavlov, Igor N.
Sakovich, Gennady V.
author_sort Shavyrkina, Nadezhda A.
collection PubMed
description One of the ways to enhance the yield of bacterial cellulose (BC) is by using dynamic aeration and different-type bioreactors because the microbial producers are strict aerobes. But in this case, the BC quality tends to worsen. Here we have combined static culture with aeration in the biosynthesis of BC by symbiotic Medusomyces gisevii Sa-12 for the first time. A new aeration method by feeding the air onto the growth medium surface is proposed herein. The culture was performed in a Binder-400 climate chamber. The study found that the air feed at a rate of 6.3 L/min allows a 25% increase in the BC yield. Moreover, this aeration mode resulted in BC samples of stable quality. The thermogravimetric and X-ray structural characteristics were retained: the crystallinity index in reflection and transmission geometries were 89% and 92%, respectively, and the allomorph Iα content was 94%. Slight decreases in the degree of polymerization (by 12.0% compared to the control―no aeration) and elastic modulus (by 12.6%) are not critical. Thus, the simple aeration by feeding the air onto the culture medium surface has turned out to be an excellent alternative to dynamic aeration. Usually, when the cultivation conditions, including the aeration ones, are changed, characteristics of the resultant BC are altered either, due to the sensitivity of individual microbial strains. In our case, the stable parameters of BC samples under variable aeration conditions are explained by the concomitant factors: the new efficient aeration method and the highly adaptive microbial producer―symbiotic Medusomyces gisevii Sa-12.
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spelling pubmed-86596262021-12-10 Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose Shavyrkina, Nadezhda A. Skiba, Ekaterina A. Kazantseva, Anastasia E. Gladysheva, Evgenia K. Budaeva, Vera V. Bychin, Nikolay V. Gismatulina, Yulia A. Kashcheyeva, Ekaterina I. Mironova, Galina F. Korchagina, Anna A. Pavlov, Igor N. Sakovich, Gennady V. Polymers (Basel) Article One of the ways to enhance the yield of bacterial cellulose (BC) is by using dynamic aeration and different-type bioreactors because the microbial producers are strict aerobes. But in this case, the BC quality tends to worsen. Here we have combined static culture with aeration in the biosynthesis of BC by symbiotic Medusomyces gisevii Sa-12 for the first time. A new aeration method by feeding the air onto the growth medium surface is proposed herein. The culture was performed in a Binder-400 climate chamber. The study found that the air feed at a rate of 6.3 L/min allows a 25% increase in the BC yield. Moreover, this aeration mode resulted in BC samples of stable quality. The thermogravimetric and X-ray structural characteristics were retained: the crystallinity index in reflection and transmission geometries were 89% and 92%, respectively, and the allomorph Iα content was 94%. Slight decreases in the degree of polymerization (by 12.0% compared to the control―no aeration) and elastic modulus (by 12.6%) are not critical. Thus, the simple aeration by feeding the air onto the culture medium surface has turned out to be an excellent alternative to dynamic aeration. Usually, when the cultivation conditions, including the aeration ones, are changed, characteristics of the resultant BC are altered either, due to the sensitivity of individual microbial strains. In our case, the stable parameters of BC samples under variable aeration conditions are explained by the concomitant factors: the new efficient aeration method and the highly adaptive microbial producer―symbiotic Medusomyces gisevii Sa-12. MDPI 2021-12-03 /pmc/articles/PMC8659626/ /pubmed/34883747 http://dx.doi.org/10.3390/polym13234241 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shavyrkina, Nadezhda A.
Skiba, Ekaterina A.
Kazantseva, Anastasia E.
Gladysheva, Evgenia K.
Budaeva, Vera V.
Bychin, Nikolay V.
Gismatulina, Yulia A.
Kashcheyeva, Ekaterina I.
Mironova, Galina F.
Korchagina, Anna A.
Pavlov, Igor N.
Sakovich, Gennady V.
Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose
title Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose
title_full Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose
title_fullStr Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose
title_full_unstemmed Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose
title_short Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose
title_sort static culture combined with aeration in biosynthesis of bacterial cellulose
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659626/
https://www.ncbi.nlm.nih.gov/pubmed/34883747
http://dx.doi.org/10.3390/polym13234241
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