Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay

DNA cyclization assay together with single-molecule FRET was employed to monitor protein-mediated bending of a short dsDNA (~ 100 bp). This method provides a simple and easy way to monitor the structural change of DNA in real-time without necessitating prior knowledge of the molecular structures for...

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Autores principales: Lee, Jae Jin, Kim, Sung Hyun, Lee, Keon Ah, Chuon, Kimleng, Jung, Kwang-Hwan, Kim, Doseok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8660804/
https://www.ncbi.nlm.nih.gov/pubmed/34887464
http://dx.doi.org/10.1038/s41598-021-03148-4
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author Lee, Jae Jin
Kim, Sung Hyun
Lee, Keon Ah
Chuon, Kimleng
Jung, Kwang-Hwan
Kim, Doseok
author_facet Lee, Jae Jin
Kim, Sung Hyun
Lee, Keon Ah
Chuon, Kimleng
Jung, Kwang-Hwan
Kim, Doseok
author_sort Lee, Jae Jin
collection PubMed
description DNA cyclization assay together with single-molecule FRET was employed to monitor protein-mediated bending of a short dsDNA (~ 100 bp). This method provides a simple and easy way to monitor the structural change of DNA in real-time without necessitating prior knowledge of the molecular structures for the optimal dye-labeling. This assay was applied to study how Anabaena sensory rhodopsin transducer (ASRT) facilitates loop formation of DNA as a possible mechanism for gene regulation. The ASRT-induced DNA looping was maximized at 50 mM of Na(+), while Mg(2+) also played an essential role in the loop formation.
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spelling pubmed-86608042021-12-13 Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay Lee, Jae Jin Kim, Sung Hyun Lee, Keon Ah Chuon, Kimleng Jung, Kwang-Hwan Kim, Doseok Sci Rep Article DNA cyclization assay together with single-molecule FRET was employed to monitor protein-mediated bending of a short dsDNA (~ 100 bp). This method provides a simple and easy way to monitor the structural change of DNA in real-time without necessitating prior knowledge of the molecular structures for the optimal dye-labeling. This assay was applied to study how Anabaena sensory rhodopsin transducer (ASRT) facilitates loop formation of DNA as a possible mechanism for gene regulation. The ASRT-induced DNA looping was maximized at 50 mM of Na(+), while Mg(2+) also played an essential role in the loop formation. Nature Publishing Group UK 2021-12-09 /pmc/articles/PMC8660804/ /pubmed/34887464 http://dx.doi.org/10.1038/s41598-021-03148-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lee, Jae Jin
Kim, Sung Hyun
Lee, Keon Ah
Chuon, Kimleng
Jung, Kwang-Hwan
Kim, Doseok
Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay
title Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay
title_full Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay
title_fullStr Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay
title_full_unstemmed Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay
title_short Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay
title_sort kinetics of dna looping by anabaena sensory rhodopsin transducer (asrt) by using dna cyclization assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8660804/
https://www.ncbi.nlm.nih.gov/pubmed/34887464
http://dx.doi.org/10.1038/s41598-021-03148-4
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