Cargando…

Reducing Phenanthrene Contamination in Trifolium repens L. With Root-Associated Phenanthrene-Degrading Bacterium Diaphorobacter sp. Phe15

Some root-associated bacteria could degrade polycyclic aromatic hydrocarbons (PAHs) in contaminated soil; however, their dynamic distribution and performance on root surface and in inner plant tissues are still unclear. In this study, greenhouse container experiments were conducted by inoculating th...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Hui, Gu, Yujun, Liu, Xiangyu, Liu, Juan, Waigi, Michael Gatheru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8660855/
https://www.ncbi.nlm.nih.gov/pubmed/34899673
http://dx.doi.org/10.3389/fmicb.2021.792698
_version_ 1784613280194494464
author Zhao, Hui
Gu, Yujun
Liu, Xiangyu
Liu, Juan
Waigi, Michael Gatheru
author_facet Zhao, Hui
Gu, Yujun
Liu, Xiangyu
Liu, Juan
Waigi, Michael Gatheru
author_sort Zhao, Hui
collection PubMed
description Some root-associated bacteria could degrade polycyclic aromatic hydrocarbons (PAHs) in contaminated soil; however, their dynamic distribution and performance on root surface and in inner plant tissues are still unclear. In this study, greenhouse container experiments were conducted by inoculating the phenanthrene-degrading bacterium Diaphorobacter sp. Phe15, which was isolated from root surfaces of healthy plants contaminated with PAHs, with the white clover (Trifolium repens L.) via root irrigation or seed soaking. The dynamic colonization, distribution, and performance of Phe15 in white clover were investigated. Strain Phe15 could efficiently degrade phenanthrene in shaking flasks and produce IAA and siderophore. After cultivation for 30, 40, and 50 days, it could colonize the root surface of white clover by forming aggregates and enter its inner tissues via root irrigation or seed soaking. The number of strain Phe15 colonized on the white clover root surfaces was the highest, reaching 6.03 Log CFU⋅g(–1) FW, followed by that in the roots and the least in the shoots. Colonization of Phe15 significantly reduced the contents of phenanthrene in white clover; the contents of phenanthrene in Phe15-inoculated plants roots and shoots were reduced by 29.92–43.16 and 41.36–51.29%, respectively, compared with the Phe15-free treatment. The Phe15 colonization also significantly enhanced the phenanthrene removal from rhizosphere soil. The colonization and performance of strain Phe15 in white clove inoculated via root inoculation were better than seed soaking. This study provides the technical support and the resource of strains for reducing the plant PAH pollution in PAH-contaminated areas.
format Online
Article
Text
id pubmed-8660855
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86608552021-12-11 Reducing Phenanthrene Contamination in Trifolium repens L. With Root-Associated Phenanthrene-Degrading Bacterium Diaphorobacter sp. Phe15 Zhao, Hui Gu, Yujun Liu, Xiangyu Liu, Juan Waigi, Michael Gatheru Front Microbiol Microbiology Some root-associated bacteria could degrade polycyclic aromatic hydrocarbons (PAHs) in contaminated soil; however, their dynamic distribution and performance on root surface and in inner plant tissues are still unclear. In this study, greenhouse container experiments were conducted by inoculating the phenanthrene-degrading bacterium Diaphorobacter sp. Phe15, which was isolated from root surfaces of healthy plants contaminated with PAHs, with the white clover (Trifolium repens L.) via root irrigation or seed soaking. The dynamic colonization, distribution, and performance of Phe15 in white clover were investigated. Strain Phe15 could efficiently degrade phenanthrene in shaking flasks and produce IAA and siderophore. After cultivation for 30, 40, and 50 days, it could colonize the root surface of white clover by forming aggregates and enter its inner tissues via root irrigation or seed soaking. The number of strain Phe15 colonized on the white clover root surfaces was the highest, reaching 6.03 Log CFU⋅g(–1) FW, followed by that in the roots and the least in the shoots. Colonization of Phe15 significantly reduced the contents of phenanthrene in white clover; the contents of phenanthrene in Phe15-inoculated plants roots and shoots were reduced by 29.92–43.16 and 41.36–51.29%, respectively, compared with the Phe15-free treatment. The Phe15 colonization also significantly enhanced the phenanthrene removal from rhizosphere soil. The colonization and performance of strain Phe15 in white clove inoculated via root inoculation were better than seed soaking. This study provides the technical support and the resource of strains for reducing the plant PAH pollution in PAH-contaminated areas. Frontiers Media S.A. 2021-11-26 /pmc/articles/PMC8660855/ /pubmed/34899673 http://dx.doi.org/10.3389/fmicb.2021.792698 Text en Copyright © 2021 Zhao, Gu, Liu, Liu and Waigi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhao, Hui
Gu, Yujun
Liu, Xiangyu
Liu, Juan
Waigi, Michael Gatheru
Reducing Phenanthrene Contamination in Trifolium repens L. With Root-Associated Phenanthrene-Degrading Bacterium Diaphorobacter sp. Phe15
title Reducing Phenanthrene Contamination in Trifolium repens L. With Root-Associated Phenanthrene-Degrading Bacterium Diaphorobacter sp. Phe15
title_full Reducing Phenanthrene Contamination in Trifolium repens L. With Root-Associated Phenanthrene-Degrading Bacterium Diaphorobacter sp. Phe15
title_fullStr Reducing Phenanthrene Contamination in Trifolium repens L. With Root-Associated Phenanthrene-Degrading Bacterium Diaphorobacter sp. Phe15
title_full_unstemmed Reducing Phenanthrene Contamination in Trifolium repens L. With Root-Associated Phenanthrene-Degrading Bacterium Diaphorobacter sp. Phe15
title_short Reducing Phenanthrene Contamination in Trifolium repens L. With Root-Associated Phenanthrene-Degrading Bacterium Diaphorobacter sp. Phe15
title_sort reducing phenanthrene contamination in trifolium repens l. with root-associated phenanthrene-degrading bacterium diaphorobacter sp. phe15
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8660855/
https://www.ncbi.nlm.nih.gov/pubmed/34899673
http://dx.doi.org/10.3389/fmicb.2021.792698
work_keys_str_mv AT zhaohui reducingphenanthrenecontaminationintrifoliumrepenslwithrootassociatedphenanthrenedegradingbacteriumdiaphorobacterspphe15
AT guyujun reducingphenanthrenecontaminationintrifoliumrepenslwithrootassociatedphenanthrenedegradingbacteriumdiaphorobacterspphe15
AT liuxiangyu reducingphenanthrenecontaminationintrifoliumrepenslwithrootassociatedphenanthrenedegradingbacteriumdiaphorobacterspphe15
AT liujuan reducingphenanthrenecontaminationintrifoliumrepenslwithrootassociatedphenanthrenedegradingbacteriumdiaphorobacterspphe15
AT waigimichaelgatheru reducingphenanthrenecontaminationintrifoliumrepenslwithrootassociatedphenanthrenedegradingbacteriumdiaphorobacterspphe15