A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

The most successful genetically encoded calcium indicators (GECIs) employ an intensity or ratiometric readout. Despite a large calcium-dependent change in fluorescence intensity, the quantification of calcium concentrations with GECIs is problematic, which is further complicated by the sensitivity o...

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Detalles Bibliográficos
Autores principales: van der Linden, Franka H., Mahlandt, Eike K., Arts, Janine J. G., Beumer, Joep, Puschhof, Jens, de Man, Saskia M. A., Chertkova, Anna O., Ponsioen, Bas, Clevers, Hans, van Buul, Jaap D., Postma, Marten, Gadella, Theodorus W. J., Goedhart, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8660884/
https://www.ncbi.nlm.nih.gov/pubmed/34887382
http://dx.doi.org/10.1038/s41467-021-27249-w

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