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Bioinformatics analysis of tumor-educated platelet microRNAs in patients with hepatocellular carcinoma

Background: Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies that seriously threaten global health. The primary reason for its grim prognosis is the lack of sensitive tools for early diagnosis. The purpose of the present study was to apply bioinformatics analysis to explore t...

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Autores principales: Zhu, Beibei, Gu, Shanshan, Wu, Xiaoting, He, Wenyong, Zhou, Hongke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8661502/
https://www.ncbi.nlm.nih.gov/pubmed/34806748
http://dx.doi.org/10.1042/BSR20211420
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author Zhu, Beibei
Gu, Shanshan
Wu, Xiaoting
He, Wenyong
Zhou, Hongke
author_facet Zhu, Beibei
Gu, Shanshan
Wu, Xiaoting
He, Wenyong
Zhou, Hongke
author_sort Zhu, Beibei
collection PubMed
description Background: Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies that seriously threaten global health. The primary reason for its grim prognosis is the lack of sensitive tools for early diagnosis. The purpose of the present study was to apply bioinformatics analysis to explore tumor-educated platelet (TEP) microRNA (miRNA) expression and its potential diagnostic utility in HCC. Methods: Twenty-five HCC patients and 25 healthy controls were included. RNA sequencing was utilized to screen miRNA alterations in platelets derived from HCC patients (n=5) and controls (n=5). Gene set enrichment analysis was performed to analyze the targeted mRNAs of differentially expressed miRNAs by using the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, aiming at main functions and pathways, respectively. We then verified the selected platelet miRNAs in another cohort by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) amplification. Results: A total of 250 differentially expressed miRNAs were identified, among which 111 were down-regulated and 139 were up-regulated. The functional enrichment analysis of differentially expressed miRNAs suggested that their target genes were involved primarily in pathways related to HCC. Expression levels of miR-495-3p and miR-1293 were further validated by qRT-PCR, which yielded results consistent with the sequencing analysis. The area under the receiver operating characteristic (ROC) curve of miR-495-3p and miR-1293 as diagnostic tests for HCC were 0.76 and 0.78, respectively. Conclusion: TEP miRNAs such as miR-495-3p and miR-1293 were differentially expressed in HCC patients, and may be involved in the pathophysiology of HCC.
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spelling pubmed-86615022021-12-21 Bioinformatics analysis of tumor-educated platelet microRNAs in patients with hepatocellular carcinoma Zhu, Beibei Gu, Shanshan Wu, Xiaoting He, Wenyong Zhou, Hongke Biosci Rep Bioinformatics Background: Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies that seriously threaten global health. The primary reason for its grim prognosis is the lack of sensitive tools for early diagnosis. The purpose of the present study was to apply bioinformatics analysis to explore tumor-educated platelet (TEP) microRNA (miRNA) expression and its potential diagnostic utility in HCC. Methods: Twenty-five HCC patients and 25 healthy controls were included. RNA sequencing was utilized to screen miRNA alterations in platelets derived from HCC patients (n=5) and controls (n=5). Gene set enrichment analysis was performed to analyze the targeted mRNAs of differentially expressed miRNAs by using the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, aiming at main functions and pathways, respectively. We then verified the selected platelet miRNAs in another cohort by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) amplification. Results: A total of 250 differentially expressed miRNAs were identified, among which 111 were down-regulated and 139 were up-regulated. The functional enrichment analysis of differentially expressed miRNAs suggested that their target genes were involved primarily in pathways related to HCC. Expression levels of miR-495-3p and miR-1293 were further validated by qRT-PCR, which yielded results consistent with the sequencing analysis. The area under the receiver operating characteristic (ROC) curve of miR-495-3p and miR-1293 as diagnostic tests for HCC were 0.76 and 0.78, respectively. Conclusion: TEP miRNAs such as miR-495-3p and miR-1293 were differentially expressed in HCC patients, and may be involved in the pathophysiology of HCC. Portland Press Ltd. 2021-12-08 /pmc/articles/PMC8661502/ /pubmed/34806748 http://dx.doi.org/10.1042/BSR20211420 Text en © 2021 The Author(s). https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Bioinformatics
Zhu, Beibei
Gu, Shanshan
Wu, Xiaoting
He, Wenyong
Zhou, Hongke
Bioinformatics analysis of tumor-educated platelet microRNAs in patients with hepatocellular carcinoma
title Bioinformatics analysis of tumor-educated platelet microRNAs in patients with hepatocellular carcinoma
title_full Bioinformatics analysis of tumor-educated platelet microRNAs in patients with hepatocellular carcinoma
title_fullStr Bioinformatics analysis of tumor-educated platelet microRNAs in patients with hepatocellular carcinoma
title_full_unstemmed Bioinformatics analysis of tumor-educated platelet microRNAs in patients with hepatocellular carcinoma
title_short Bioinformatics analysis of tumor-educated platelet microRNAs in patients with hepatocellular carcinoma
title_sort bioinformatics analysis of tumor-educated platelet micrornas in patients with hepatocellular carcinoma
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8661502/
https://www.ncbi.nlm.nih.gov/pubmed/34806748
http://dx.doi.org/10.1042/BSR20211420
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