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Flow cytometry-based quantification of targeted knock-in events in human cell lines using a GPI-anchor biosynthesis gene PIGP

Targeted knock-in supported by the CRISPR/Cas systems enables the insertion, deletion, and substitution of genome sequences exactly as designed. Although this technology is considered to have wide range of applications in life sciences, one of its prerequisites for practical use is to improve the ef...

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Detalles Bibliográficos
Autores principales:	Rahman, Md. Lutfur, Hyodo, Toshinori, Hasan, Muhammad Nazmul, Mihara, Yuko, Karnan, Sivasundaram, Ota, Akinobu, Tsuzuki, Shinobu, Hosokawa, Yoshitaka, Konishi, Hiroyuki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Portland Press Ltd. 2021
Materias:	Biochemical Techniques & Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8661509/ https://www.ncbi.nlm.nih.gov/pubmed/34750615 http://dx.doi.org/10.1042/BSR20212231

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