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RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer

RNA N6-methyladenosine (m6A) methylation is known to be the most popular RNA modification in animals. Many research reports have elaborated on the effects of m6A regulators in medical practice, such as diagnosis, prognosis, and treatment. M6A modification has evident impacts on many aspects of RNA m...

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Detalles Bibliográficos
Autores principales: Su, Hao, Wang, Yutao, Li, Hongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8661905/
https://www.ncbi.nlm.nih.gov/pubmed/34899855
http://dx.doi.org/10.3389/fgene.2021.768041
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author Su, Hao
Wang, Yutao
Li, Hongjun
author_facet Su, Hao
Wang, Yutao
Li, Hongjun
author_sort Su, Hao
collection PubMed
description RNA N6-methyladenosine (m6A) methylation is known to be the most popular RNA modification in animals. Many research reports have elaborated on the effects of m6A regulators in medical practice, such as diagnosis, prognosis, and treatment. M6A modification has evident impacts on many aspects of RNA metabolism, just like RNA splicing, processing, translation, and stability. M6A also has a magnificent role in numerous types of cancers. We analyzed the prostate cancer datasets, from The Cancer Genome Atlas (TCGA) database, for every recognized m6A regulator in their gene expression, DNA methylation status and copy number variations (CNVs). We also systematically analyzed the relationship between different m6A regulators and the prognosis of prostate cancer. The results illustrated considerable differences in the expression of various m6A regulators between the prostate and normal cancer samples. At the same time, there were evident differences in the expression of various m6A regulators in prostate cancers with different Gleason scores. Subsequently, we determined CBLL1, FTO, YTHDC1, HNRNPA2B1 as crucial m6A regulators of prostate cancer. Premised on the expression of CBLL1, we also identified potential therapeutic agents for prostate cancer, and knockdown of FTO prominently inhibited prostate cells migration and invasion in vitro experiment.
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spelling pubmed-86619052021-12-11 RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer Su, Hao Wang, Yutao Li, Hongjun Front Genet Genetics RNA N6-methyladenosine (m6A) methylation is known to be the most popular RNA modification in animals. Many research reports have elaborated on the effects of m6A regulators in medical practice, such as diagnosis, prognosis, and treatment. M6A modification has evident impacts on many aspects of RNA metabolism, just like RNA splicing, processing, translation, and stability. M6A also has a magnificent role in numerous types of cancers. We analyzed the prostate cancer datasets, from The Cancer Genome Atlas (TCGA) database, for every recognized m6A regulator in their gene expression, DNA methylation status and copy number variations (CNVs). We also systematically analyzed the relationship between different m6A regulators and the prognosis of prostate cancer. The results illustrated considerable differences in the expression of various m6A regulators between the prostate and normal cancer samples. At the same time, there were evident differences in the expression of various m6A regulators in prostate cancers with different Gleason scores. Subsequently, we determined CBLL1, FTO, YTHDC1, HNRNPA2B1 as crucial m6A regulators of prostate cancer. Premised on the expression of CBLL1, we also identified potential therapeutic agents for prostate cancer, and knockdown of FTO prominently inhibited prostate cells migration and invasion in vitro experiment. Frontiers Media S.A. 2021-11-26 /pmc/articles/PMC8661905/ /pubmed/34899855 http://dx.doi.org/10.3389/fgene.2021.768041 Text en Copyright © 2021 Su, Wang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Su, Hao
Wang, Yutao
Li, Hongjun
RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer
title RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer
title_full RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer
title_fullStr RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer
title_full_unstemmed RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer
title_short RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer
title_sort rna m6a methylation regulators multi-omics analysis in prostate cancer
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8661905/
https://www.ncbi.nlm.nih.gov/pubmed/34899855
http://dx.doi.org/10.3389/fgene.2021.768041
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