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Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury
Our study investigates the potential of diffusion MRI (dMRI), including diffusion tensor imaging (DTI), fixel-based analysis (FBA) and neurite orientation dispersion and density imaging (NODDI), to detect microstructural tissue abnormalities in rats after mild traumatic brain injury (mTBI). The brai...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662623/ https://www.ncbi.nlm.nih.gov/pubmed/34899158 http://dx.doi.org/10.3389/fnins.2021.746214 |
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author | Chary, Karthik Narvaez, Omar Salo, Raimo A. San Martín Molina, Isabel Tohka, Jussi Aggarwal, Manisha Gröhn, Olli Sierra, Alejandra |
author_facet | Chary, Karthik Narvaez, Omar Salo, Raimo A. San Martín Molina, Isabel Tohka, Jussi Aggarwal, Manisha Gröhn, Olli Sierra, Alejandra |
author_sort | Chary, Karthik |
collection | PubMed |
description | Our study investigates the potential of diffusion MRI (dMRI), including diffusion tensor imaging (DTI), fixel-based analysis (FBA) and neurite orientation dispersion and density imaging (NODDI), to detect microstructural tissue abnormalities in rats after mild traumatic brain injury (mTBI). The brains of sham-operated and mTBI rats 35 days after lateral fluid percussion injury were imaged ex vivo in a 11.7-T scanner. Voxel-based analyses of DTI-, fixel- and NODDI-based metrics detected extensive tissue changes in directly affected brain areas close to the primary injury, and more importantly, also in distal areas connected to primary injury and indirectly affected by the secondary injury mechanisms. Histology revealed ongoing axonal abnormalities and inflammation, 35 days after the injury, in the brain areas highlighted in the group analyses. Fractional anisotropy (FA), fiber density (FD) and fiber density and fiber bundle cross-section (FDC) showed similar pattern of significant areas throughout the brain; however, FA showed more significant voxels in gray matter areas, while FD and FDC in white matter areas, and orientation dispersion index (ODI) in areas most damage based on histology. Region-of-interest (ROI)-based analyses on dMRI maps and histology in selected brain regions revealed that the changes in MRI parameters could be attributed to both alterations in myelinated fiber bundles and increased cellularity. This study demonstrates that the combination of dMRI methods can provide a more complete insight into the microstructural alterations in white and gray matter after mTBI, which may aid diagnosis and prognosis following a mild brain injury. |
format | Online Article Text |
id | pubmed-8662623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86626232021-12-11 Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury Chary, Karthik Narvaez, Omar Salo, Raimo A. San Martín Molina, Isabel Tohka, Jussi Aggarwal, Manisha Gröhn, Olli Sierra, Alejandra Front Neurosci Neuroscience Our study investigates the potential of diffusion MRI (dMRI), including diffusion tensor imaging (DTI), fixel-based analysis (FBA) and neurite orientation dispersion and density imaging (NODDI), to detect microstructural tissue abnormalities in rats after mild traumatic brain injury (mTBI). The brains of sham-operated and mTBI rats 35 days after lateral fluid percussion injury were imaged ex vivo in a 11.7-T scanner. Voxel-based analyses of DTI-, fixel- and NODDI-based metrics detected extensive tissue changes in directly affected brain areas close to the primary injury, and more importantly, also in distal areas connected to primary injury and indirectly affected by the secondary injury mechanisms. Histology revealed ongoing axonal abnormalities and inflammation, 35 days after the injury, in the brain areas highlighted in the group analyses. Fractional anisotropy (FA), fiber density (FD) and fiber density and fiber bundle cross-section (FDC) showed similar pattern of significant areas throughout the brain; however, FA showed more significant voxels in gray matter areas, while FD and FDC in white matter areas, and orientation dispersion index (ODI) in areas most damage based on histology. Region-of-interest (ROI)-based analyses on dMRI maps and histology in selected brain regions revealed that the changes in MRI parameters could be attributed to both alterations in myelinated fiber bundles and increased cellularity. This study demonstrates that the combination of dMRI methods can provide a more complete insight into the microstructural alterations in white and gray matter after mTBI, which may aid diagnosis and prognosis following a mild brain injury. Frontiers Media S.A. 2021-11-26 /pmc/articles/PMC8662623/ /pubmed/34899158 http://dx.doi.org/10.3389/fnins.2021.746214 Text en Copyright © 2021 Chary, Narvaez, Salo, San Martín Molina, Tohka, Aggarwal, Gröhn and Sierra. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Chary, Karthik Narvaez, Omar Salo, Raimo A. San Martín Molina, Isabel Tohka, Jussi Aggarwal, Manisha Gröhn, Olli Sierra, Alejandra Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury |
title | Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury |
title_full | Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury |
title_fullStr | Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury |
title_full_unstemmed | Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury |
title_short | Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury |
title_sort | microstructural tissue changes in a rat model of mild traumatic brain injury |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662623/ https://www.ncbi.nlm.nih.gov/pubmed/34899158 http://dx.doi.org/10.3389/fnins.2021.746214 |
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