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Streptavidin Coverage on Biotinylated Surfaces

[Image: see text] Biosensors and other biological platform technologies require the functionalization of their surface with receptors to enhance affinity and selectivity. Control over the functionalization density is required to tune the platform’s properties. Streptavidin (SAv) monolayers are widel...

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Autores principales: Hamming, P. H. Erik, Huskens, Jurriaan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662640/
https://www.ncbi.nlm.nih.gov/pubmed/34813287
http://dx.doi.org/10.1021/acsami.1c16446
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author Hamming, P. H. Erik
Huskens, Jurriaan
author_facet Hamming, P. H. Erik
Huskens, Jurriaan
author_sort Hamming, P. H. Erik
collection PubMed
description [Image: see text] Biosensors and other biological platform technologies require the functionalization of their surface with receptors to enhance affinity and selectivity. Control over the functionalization density is required to tune the platform’s properties. Streptavidin (SAv) monolayers are widely used to immobilize biotinylated proteins, receptors, and DNA. The SAv density on a surface can be varied easily, but the predictability is dependent on the method by which the SAv is immobilized. In this study we show a method to quantitatively predict the SAv coverage on biotinylated surfaces. The method is validated by measuring the SAv coverage on supported lipid bilayers with a range of biotin contents and two different main phase lipids and by using quartz crystal microbalance and localized surface plasmon resonance. We explore a predictive model of the biotin-dependent SAv coverage without any fit parameters. Model and data allow to predict the SAv coverage based on the biotin coverage, in both the low- and high-density regimes. This is of special importance in applications with multivalent binding where control over surface receptor density is required, but a direct measurement is not possible.
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spelling pubmed-86626402021-12-10 Streptavidin Coverage on Biotinylated Surfaces Hamming, P. H. Erik Huskens, Jurriaan ACS Appl Mater Interfaces [Image: see text] Biosensors and other biological platform technologies require the functionalization of their surface with receptors to enhance affinity and selectivity. Control over the functionalization density is required to tune the platform’s properties. Streptavidin (SAv) monolayers are widely used to immobilize biotinylated proteins, receptors, and DNA. The SAv density on a surface can be varied easily, but the predictability is dependent on the method by which the SAv is immobilized. In this study we show a method to quantitatively predict the SAv coverage on biotinylated surfaces. The method is validated by measuring the SAv coverage on supported lipid bilayers with a range of biotin contents and two different main phase lipids and by using quartz crystal microbalance and localized surface plasmon resonance. We explore a predictive model of the biotin-dependent SAv coverage without any fit parameters. Model and data allow to predict the SAv coverage based on the biotin coverage, in both the low- and high-density regimes. This is of special importance in applications with multivalent binding where control over surface receptor density is required, but a direct measurement is not possible. American Chemical Society 2021-11-23 2021-12-08 /pmc/articles/PMC8662640/ /pubmed/34813287 http://dx.doi.org/10.1021/acsami.1c16446 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Hamming, P. H. Erik
Huskens, Jurriaan
Streptavidin Coverage on Biotinylated Surfaces
title Streptavidin Coverage on Biotinylated Surfaces
title_full Streptavidin Coverage on Biotinylated Surfaces
title_fullStr Streptavidin Coverage on Biotinylated Surfaces
title_full_unstemmed Streptavidin Coverage on Biotinylated Surfaces
title_short Streptavidin Coverage on Biotinylated Surfaces
title_sort streptavidin coverage on biotinylated surfaces
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662640/
https://www.ncbi.nlm.nih.gov/pubmed/34813287
http://dx.doi.org/10.1021/acsami.1c16446
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