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Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce

The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great...

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Autores principales: Darqui, Flavia Soledad, Radonic, Laura Mabel, Beracochea, Valeria Cecilia, Hopp, H. Esteban, López Bilbao, Marisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662702/
https://www.ncbi.nlm.nih.gov/pubmed/34899788
http://dx.doi.org/10.3389/fpls.2021.767459
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author Darqui, Flavia Soledad
Radonic, Laura Mabel
Beracochea, Valeria Cecilia
Hopp, H. Esteban
López Bilbao, Marisa
author_facet Darqui, Flavia Soledad
Radonic, Laura Mabel
Beracochea, Valeria Cecilia
Hopp, H. Esteban
López Bilbao, Marisa
author_sort Darqui, Flavia Soledad
collection PubMed
description The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.
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spelling pubmed-86627022021-12-11 Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce Darqui, Flavia Soledad Radonic, Laura Mabel Beracochea, Valeria Cecilia Hopp, H. Esteban López Bilbao, Marisa Front Plant Sci Plant Science The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species. Frontiers Media S.A. 2021-11-26 /pmc/articles/PMC8662702/ /pubmed/34899788 http://dx.doi.org/10.3389/fpls.2021.767459 Text en Copyright © 2021 Darqui, Radonic, Beracochea, Hopp and López Bilbao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Darqui, Flavia Soledad
Radonic, Laura Mabel
Beracochea, Valeria Cecilia
Hopp, H. Esteban
López Bilbao, Marisa
Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_full Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_fullStr Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_full_unstemmed Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_short Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_sort peculiarities of the transformation of asteraceae family species: the cases of sunflower and lettuce
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662702/
https://www.ncbi.nlm.nih.gov/pubmed/34899788
http://dx.doi.org/10.3389/fpls.2021.767459
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