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Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting

Background: Nanometer-sized membrane-surrounded vesicles from different parts of plants including fruits are gaining increasing attention due to their anti-inflammatory and anticancer effects demonstrated by in vitro and in vivo studies, and as nanovectors for molecular delivery of exogenous substan...

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Autores principales: Stanly, Christopher, Kim, Hyoseon, Antonucci, Giuseppe, Fiume, Immacolata, Guescini, Michele, Kim, Kwang Pyo, Ciardiello, Maria Antonietta, Giangrieco, Ivana, Mari, Adriano, Pocsfalvi, Gabriella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662998/
https://www.ncbi.nlm.nih.gov/pubmed/34900959
http://dx.doi.org/10.3389/fbioe.2021.760730
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author Stanly, Christopher
Kim, Hyoseon
Antonucci, Giuseppe
Fiume, Immacolata
Guescini, Michele
Kim, Kwang Pyo
Ciardiello, Maria Antonietta
Giangrieco, Ivana
Mari, Adriano
Pocsfalvi, Gabriella
author_facet Stanly, Christopher
Kim, Hyoseon
Antonucci, Giuseppe
Fiume, Immacolata
Guescini, Michele
Kim, Kwang Pyo
Ciardiello, Maria Antonietta
Giangrieco, Ivana
Mari, Adriano
Pocsfalvi, Gabriella
author_sort Stanly, Christopher
collection PubMed
description Background: Nanometer-sized membrane-surrounded vesicles from different parts of plants including fruits are gaining increasing attention due to their anti-inflammatory and anticancer effects demonstrated by in vitro and in vivo studies, and as nanovectors for molecular delivery of exogenous substances. These nanomaterials are very complex and contain a diverse arsenal of bioactive molecules, such as nucleic acids, proteins, and lipids. Our knowledge about the transport of allergens in vesicles isolated from plant food is limited today. Methods: Here, to investigate the allergenicity of strawberry-derived microvesicles (MVs), nanovesicles (NVs), and subpopulations of NV, we have set up a multidisciplinary approach. The strategy combines proteomics-based protein identification, immunological investigations, bioinformatics, and data mining to gain biological insights useful to evaluate the presence of potential allergens and the immunoglobulin E (IgE) inhibitory activity of vesicle preparations. Results: Immunological test showed that several proteins of strawberry-derived vesicles compete for IgE binding with allergens spotted on the FABER biochip. This includes the known strawberry allergens Fra a 1, Fra a 3, and Fra a 4, and also other IgE-binding proteins not yet described as allergens in this food, such as gibberellin-regulated proteins, 2S albumin, pectate lyase, and trypsin inhibitors. Proteomics identified homologous sequences of the three strawberry allergens and their isoforms in total protein extract (TPE) but only Fra a 1 and Fra a 4 in the vesicle samples. Label-free quantitative proteomic analysis revealed no significant enrichment of these proteins in strawberry vesicles with respect to TPE. Conclusion: Immunological tests and bioinformatics analysis of proteomics data sets revealed that MVs and NVs isolated from strawberries can carry functional allergens their isoforms as well as proteins potentially allergenic based on their structural features. This should be considered when these new nanomaterials are used for human nutraceutical or biomedical applications.
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spelling pubmed-86629982021-12-11 Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting Stanly, Christopher Kim, Hyoseon Antonucci, Giuseppe Fiume, Immacolata Guescini, Michele Kim, Kwang Pyo Ciardiello, Maria Antonietta Giangrieco, Ivana Mari, Adriano Pocsfalvi, Gabriella Front Bioeng Biotechnol Bioengineering and Biotechnology Background: Nanometer-sized membrane-surrounded vesicles from different parts of plants including fruits are gaining increasing attention due to their anti-inflammatory and anticancer effects demonstrated by in vitro and in vivo studies, and as nanovectors for molecular delivery of exogenous substances. These nanomaterials are very complex and contain a diverse arsenal of bioactive molecules, such as nucleic acids, proteins, and lipids. Our knowledge about the transport of allergens in vesicles isolated from plant food is limited today. Methods: Here, to investigate the allergenicity of strawberry-derived microvesicles (MVs), nanovesicles (NVs), and subpopulations of NV, we have set up a multidisciplinary approach. The strategy combines proteomics-based protein identification, immunological investigations, bioinformatics, and data mining to gain biological insights useful to evaluate the presence of potential allergens and the immunoglobulin E (IgE) inhibitory activity of vesicle preparations. Results: Immunological test showed that several proteins of strawberry-derived vesicles compete for IgE binding with allergens spotted on the FABER biochip. This includes the known strawberry allergens Fra a 1, Fra a 3, and Fra a 4, and also other IgE-binding proteins not yet described as allergens in this food, such as gibberellin-regulated proteins, 2S albumin, pectate lyase, and trypsin inhibitors. Proteomics identified homologous sequences of the three strawberry allergens and their isoforms in total protein extract (TPE) but only Fra a 1 and Fra a 4 in the vesicle samples. Label-free quantitative proteomic analysis revealed no significant enrichment of these proteins in strawberry vesicles with respect to TPE. Conclusion: Immunological tests and bioinformatics analysis of proteomics data sets revealed that MVs and NVs isolated from strawberries can carry functional allergens their isoforms as well as proteins potentially allergenic based on their structural features. This should be considered when these new nanomaterials are used for human nutraceutical or biomedical applications. Frontiers Media S.A. 2021-11-26 /pmc/articles/PMC8662998/ /pubmed/34900959 http://dx.doi.org/10.3389/fbioe.2021.760730 Text en Copyright © 2021 Stanly, Kim, Antonucci, Fiume, Guescini, Kim, Ciardiello, Giangrieco, Mari and Pocsfalvi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Stanly, Christopher
Kim, Hyoseon
Antonucci, Giuseppe
Fiume, Immacolata
Guescini, Michele
Kim, Kwang Pyo
Ciardiello, Maria Antonietta
Giangrieco, Ivana
Mari, Adriano
Pocsfalvi, Gabriella
Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting
title Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting
title_full Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting
title_fullStr Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting
title_full_unstemmed Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting
title_short Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting
title_sort crosstalk between the immune system and plant-derived nanovesicles: a study of allergen transporting
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662998/
https://www.ncbi.nlm.nih.gov/pubmed/34900959
http://dx.doi.org/10.3389/fbioe.2021.760730
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