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Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.)

The present study analysed the molecular and agro-morphological diversity in a set of 92 diverse cauliflower genotypes and two each of cabbage and broccoli. Field evaluation of the genotypes was done in randomized block design (RBD) at two locations (i.e. IARI, New Delhi and ICAR-RC-NEH Region, Bara...

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Autores principales: Rakshita, K. N., Singh, Shrawan, Verma, Veerendra Kumar, Sharma, Brij Bihari, Saini, Navinder, Iquebal, Mir Asif, Sharma, Akanksha, Dey, Shyam Sunder, Behera, T. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664203/
https://www.ncbi.nlm.nih.gov/pubmed/34890399
http://dx.doi.org/10.1371/journal.pone.0260246
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author Rakshita, K. N.
Singh, Shrawan
Verma, Veerendra Kumar
Sharma, Brij Bihari
Saini, Navinder
Iquebal, Mir Asif
Sharma, Akanksha
Dey, Shyam Sunder
Behera, T. K.
author_facet Rakshita, K. N.
Singh, Shrawan
Verma, Veerendra Kumar
Sharma, Brij Bihari
Saini, Navinder
Iquebal, Mir Asif
Sharma, Akanksha
Dey, Shyam Sunder
Behera, T. K.
author_sort Rakshita, K. N.
collection PubMed
description The present study analysed the molecular and agro-morphological diversity in a set of 92 diverse cauliflower genotypes and two each of cabbage and broccoli. Field evaluation of the genotypes was done in randomized block design (RBD) at two locations (i.e. IARI, New Delhi and ICAR-RC-NEH Region, Barapani) during Rabi2019-20. Genotypes showed variation for all the eight observed traits at both locations and, the differences in early and snowball groups were distinct. Pusa Meghna, DC-33-8, Pusa Kartiki and CC-14 were earliest for curd initiation. Genotypes showed higher values for curd traits at Delhi. Molecular diversity was detected with 90 polymorphic simple sequence repeats (SSR). Number of alleles ranged from 1 to 9 with mean value of 2.16 and the highest polymorphic information content (PIC) value was observed for primer BoGMS0742 (0.68) with a mean value of 0.18. Cluster analysis using agro-morphological traits substantiated classification of the genotypes for maturity groups. However, SSR analysis revealed four clusters and with a composite pattern of genotype distribution. STRUCTURE analysis also supported the admixture and four subpopulations. The studyindicates for introgression of genetic fragments across the maturity groups, thereby, potential for use in further genetic improvement and heterosis breeding.
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spelling pubmed-86642032021-12-11 Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.) Rakshita, K. N. Singh, Shrawan Verma, Veerendra Kumar Sharma, Brij Bihari Saini, Navinder Iquebal, Mir Asif Sharma, Akanksha Dey, Shyam Sunder Behera, T. K. PLoS One Research Article The present study analysed the molecular and agro-morphological diversity in a set of 92 diverse cauliflower genotypes and two each of cabbage and broccoli. Field evaluation of the genotypes was done in randomized block design (RBD) at two locations (i.e. IARI, New Delhi and ICAR-RC-NEH Region, Barapani) during Rabi2019-20. Genotypes showed variation for all the eight observed traits at both locations and, the differences in early and snowball groups were distinct. Pusa Meghna, DC-33-8, Pusa Kartiki and CC-14 were earliest for curd initiation. Genotypes showed higher values for curd traits at Delhi. Molecular diversity was detected with 90 polymorphic simple sequence repeats (SSR). Number of alleles ranged from 1 to 9 with mean value of 2.16 and the highest polymorphic information content (PIC) value was observed for primer BoGMS0742 (0.68) with a mean value of 0.18. Cluster analysis using agro-morphological traits substantiated classification of the genotypes for maturity groups. However, SSR analysis revealed four clusters and with a composite pattern of genotype distribution. STRUCTURE analysis also supported the admixture and four subpopulations. The studyindicates for introgression of genetic fragments across the maturity groups, thereby, potential for use in further genetic improvement and heterosis breeding. Public Library of Science 2021-12-10 /pmc/articles/PMC8664203/ /pubmed/34890399 http://dx.doi.org/10.1371/journal.pone.0260246 Text en © 2021 Rakshita et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rakshita, K. N.
Singh, Shrawan
Verma, Veerendra Kumar
Sharma, Brij Bihari
Saini, Navinder
Iquebal, Mir Asif
Sharma, Akanksha
Dey, Shyam Sunder
Behera, T. K.
Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.)
title Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.)
title_full Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.)
title_fullStr Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.)
title_full_unstemmed Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.)
title_short Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.)
title_sort agro-morphological and molecular diversity in different maturity groups of indian cauliflower (brassica oleracea var. botrytis l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664203/
https://www.ncbi.nlm.nih.gov/pubmed/34890399
http://dx.doi.org/10.1371/journal.pone.0260246
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