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Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase

Plasmid shuttle vectors capable of replication in both Saccharomyces cerevisiae and Escherichia coli and optimized for controlled modification in vitro and in vivo are a key resource supporting yeast as a premier system for genetics research and synthetic biology. We have engineered a series of yeas...

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Detalles Bibliográficos
Autores principales: Nickerson, Daniel P, Quinn, Monique A, Milnes, Joshua M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664424/
https://www.ncbi.nlm.nih.gov/pubmed/34599813
http://dx.doi.org/10.1093/g3journal/jkab336
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author Nickerson, Daniel P
Quinn, Monique A
Milnes, Joshua M
author_facet Nickerson, Daniel P
Quinn, Monique A
Milnes, Joshua M
author_sort Nickerson, Daniel P
collection PubMed
description Plasmid shuttle vectors capable of replication in both Saccharomyces cerevisiae and Escherichia coli and optimized for controlled modification in vitro and in vivo are a key resource supporting yeast as a premier system for genetics research and synthetic biology. We have engineered a series of yeast shuttle vectors optimized for efficient insertion, removal, and substitution of plasmid yeast replication loci, allowing generation of a complete set of integrating, low copy and high copy plasmids via predictable operations as an alternative to traditional subcloning. We demonstrate the utility of this system through modification of replication loci via Cre recombinase, both in vitro and in vivo, and restriction endonuclease treatments.
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spelling pubmed-86644242021-12-13 Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase Nickerson, Daniel P Quinn, Monique A Milnes, Joshua M G3 (Bethesda) Investigation Plasmid shuttle vectors capable of replication in both Saccharomyces cerevisiae and Escherichia coli and optimized for controlled modification in vitro and in vivo are a key resource supporting yeast as a premier system for genetics research and synthetic biology. We have engineered a series of yeast shuttle vectors optimized for efficient insertion, removal, and substitution of plasmid yeast replication loci, allowing generation of a complete set of integrating, low copy and high copy plasmids via predictable operations as an alternative to traditional subcloning. We demonstrate the utility of this system through modification of replication loci via Cre recombinase, both in vitro and in vivo, and restriction endonuclease treatments. Oxford University Press 2021-10-02 /pmc/articles/PMC8664424/ /pubmed/34599813 http://dx.doi.org/10.1093/g3journal/jkab336 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Nickerson, Daniel P
Quinn, Monique A
Milnes, Joshua M
Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
title Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
title_full Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
title_fullStr Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
title_full_unstemmed Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
title_short Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
title_sort rapid conversion of replicating and integrating saccharomyces cerevisiae plasmid vectors via cre recombinase
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664424/
https://www.ncbi.nlm.nih.gov/pubmed/34599813
http://dx.doi.org/10.1093/g3journal/jkab336
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