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Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae

Genome editing using the CRISPR/Cas system has been implemented for various organisms and becomes increasingly popular even in the genetically tractable budding yeast Saccharomyces cerevisiae. Because each CRISPR/Cas system recognizes only the sequences flanked by its unique protospacer adjacent mot...

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Detalles Bibliográficos
Autores principales:	Okada, Satoshi, Doi, Goro, Nakagawa, Shitomi, Kusumoto, Emiko, Ito, Takashi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Investigation
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664446/ https://www.ncbi.nlm.nih.gov/pubmed/34739076 http://dx.doi.org/10.1093/g3journal/jkab304

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