Pipeline for generating stable large genomic deletions in zebrafish, from small domains to whole gene excisions

Here we describe a short feasibility study and methodological framework for the production of stable, CRISPR/Cas9-based, large genomic deletions in zebrafish, ranging from several base pairs (bp) to hundreds of kilobases (kb). Using a cocktail of four single guide RNAs (sgRNAs) targeting a single ge...

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Detalles Bibliográficos
Autores principales: Tromp, Alisha, Robinson, Kate, Hall, Thomas E, Mowry, Bryan, Giacomotto, Jean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Investigation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664487/
https://www.ncbi.nlm.nih.gov/pubmed/34499171
http://dx.doi.org/10.1093/g3journal/jkab321

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664487/
https://www.ncbi.nlm.nih.gov/pubmed/34499171
http://dx.doi.org/10.1093/g3journal/jkab321

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