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Pipeline for generating stable large genomic deletions in zebrafish, from small domains to whole gene excisions
Here we describe a short feasibility study and methodological framework for the production of stable, CRISPR/Cas9-based, large genomic deletions in zebrafish, ranging from several base pairs (bp) to hundreds of kilobases (kb). Using a cocktail of four single guide RNAs (sgRNAs) targeting a single ge...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664487/ https://www.ncbi.nlm.nih.gov/pubmed/34499171 http://dx.doi.org/10.1093/g3journal/jkab321 |