Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo

To better understand the input-output computations of neuronal populations, we developed ArcLight-ST, a genetically-encoded voltage indicator, to specifically measure subthreshold membrane potentials. We combined two-photon imaging of voltage and calcium, and successfully discriminated subthreshold...

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Detalles Bibliográficos
Autores principales: Bando, Yuki, Wenzel, Michael, Yuste, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664861/
https://www.ncbi.nlm.nih.gov/pubmed/34893595
http://dx.doi.org/10.1038/s41467-021-27444-9
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author Bando, Yuki
Wenzel, Michael
Yuste, Rafael
author_facet Bando, Yuki
Wenzel, Michael
Yuste, Rafael
author_sort Bando, Yuki
collection PubMed
description To better understand the input-output computations of neuronal populations, we developed ArcLight-ST, a genetically-encoded voltage indicator, to specifically measure subthreshold membrane potentials. We combined two-photon imaging of voltage and calcium, and successfully discriminated subthreshold inputs and spikes with cellular resolution in vivo. We demonstrate the utility of the method by mapping epileptic seizures progression through cortical circuits, revealing divergent sub- and suprathreshold dynamics within compartmentalized epileptic micronetworks. Two-photon, two-color imaging of calcium and voltage enables mapping of inputs and outputs in neuronal populations in living animals.
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spelling pubmed-86648612021-12-27 Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo Bando, Yuki Wenzel, Michael Yuste, Rafael Nat Commun Article To better understand the input-output computations of neuronal populations, we developed ArcLight-ST, a genetically-encoded voltage indicator, to specifically measure subthreshold membrane potentials. We combined two-photon imaging of voltage and calcium, and successfully discriminated subthreshold inputs and spikes with cellular resolution in vivo. We demonstrate the utility of the method by mapping epileptic seizures progression through cortical circuits, revealing divergent sub- and suprathreshold dynamics within compartmentalized epileptic micronetworks. Two-photon, two-color imaging of calcium and voltage enables mapping of inputs and outputs in neuronal populations in living animals. Nature Publishing Group UK 2021-12-10 /pmc/articles/PMC8664861/ /pubmed/34893595 http://dx.doi.org/10.1038/s41467-021-27444-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bando, Yuki
Wenzel, Michael
Yuste, Rafael
Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo
title Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo
title_full Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo
title_fullStr Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo
title_full_unstemmed Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo
title_short Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo
title_sort simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664861/
https://www.ncbi.nlm.nih.gov/pubmed/34893595
http://dx.doi.org/10.1038/s41467-021-27444-9
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AT yusterafael simultaneoustwophotonimagingofactionpotentialsandsubthresholdinputsinvivo

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