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Anterograde Transneuronal Tracing and Genetic Control with Engineered Yellow Fever Vaccine YFV-17D

Transneuronal viruses are powerful tools for tracing neuronal circuits or delivering genes to specific neurons in the brain. While there are multiple retrograde viruses, few anterograde viruses are available. Further, available anterograde viruses often have limitations such as retrograde transport,...

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Autores principales: Li, Elizabeth, Guo, Jun, Oh, So Jung, Luo, Yi, Oliveros, Heankel Cantu, Du, Wenqin, Arano, Rachel, Kim, Yerim, Chen, Yuh-Tarng, Eitson, Jennifer, Lin, Da-Ting, Li, Ying, Roberts, Todd, Schoggins, John W., Xu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665090/
https://www.ncbi.nlm.nih.gov/pubmed/34824475
http://dx.doi.org/10.1038/s41592-021-01319-9
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author Li, Elizabeth
Guo, Jun
Oh, So Jung
Luo, Yi
Oliveros, Heankel Cantu
Du, Wenqin
Arano, Rachel
Kim, Yerim
Chen, Yuh-Tarng
Eitson, Jennifer
Lin, Da-Ting
Li, Ying
Roberts, Todd
Schoggins, John W.
Xu, Wei
author_facet Li, Elizabeth
Guo, Jun
Oh, So Jung
Luo, Yi
Oliveros, Heankel Cantu
Du, Wenqin
Arano, Rachel
Kim, Yerim
Chen, Yuh-Tarng
Eitson, Jennifer
Lin, Da-Ting
Li, Ying
Roberts, Todd
Schoggins, John W.
Xu, Wei
author_sort Li, Elizabeth
collection PubMed
description Transneuronal viruses are powerful tools for tracing neuronal circuits or delivering genes to specific neurons in the brain. While there are multiple retrograde viruses, few anterograde viruses are available. Further, available anterograde viruses often have limitations such as retrograde transport, high neuronal toxicity, or weak signals. We developed an anterograde viral system based on a live attenuated vaccine of yellow fever – YFV-17D. Replication-deficient or packaging-deficient mutants of YFV-17D can be reconstituted in the brain, leading to efficient synapse-specific and anterograde-only transneuronal spreading, which can be controlled to achieve either monosynaptic or polysynaptic tracing. Moreover, inducible transient replication of YFV-17D mutant is sufficient to induce permanent transneuronal genetic modifications without causing neuronal toxicity. The engineered YFV-17D systems can be used to express fluorescent markers, sensors or effectors in downstream neurons, thus providing versatile tools for mapping and functionally controlling neuronal circuits.
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spelling pubmed-86650902022-05-25 Anterograde Transneuronal Tracing and Genetic Control with Engineered Yellow Fever Vaccine YFV-17D Li, Elizabeth Guo, Jun Oh, So Jung Luo, Yi Oliveros, Heankel Cantu Du, Wenqin Arano, Rachel Kim, Yerim Chen, Yuh-Tarng Eitson, Jennifer Lin, Da-Ting Li, Ying Roberts, Todd Schoggins, John W. Xu, Wei Nat Methods Article Transneuronal viruses are powerful tools for tracing neuronal circuits or delivering genes to specific neurons in the brain. While there are multiple retrograde viruses, few anterograde viruses are available. Further, available anterograde viruses often have limitations such as retrograde transport, high neuronal toxicity, or weak signals. We developed an anterograde viral system based on a live attenuated vaccine of yellow fever – YFV-17D. Replication-deficient or packaging-deficient mutants of YFV-17D can be reconstituted in the brain, leading to efficient synapse-specific and anterograde-only transneuronal spreading, which can be controlled to achieve either monosynaptic or polysynaptic tracing. Moreover, inducible transient replication of YFV-17D mutant is sufficient to induce permanent transneuronal genetic modifications without causing neuronal toxicity. The engineered YFV-17D systems can be used to express fluorescent markers, sensors or effectors in downstream neurons, thus providing versatile tools for mapping and functionally controlling neuronal circuits. 2021-11-25 2021-12 /pmc/articles/PMC8665090/ /pubmed/34824475 http://dx.doi.org/10.1038/s41592-021-01319-9 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:
spellingShingle Article
Li, Elizabeth
Guo, Jun
Oh, So Jung
Luo, Yi
Oliveros, Heankel Cantu
Du, Wenqin
Arano, Rachel
Kim, Yerim
Chen, Yuh-Tarng
Eitson, Jennifer
Lin, Da-Ting
Li, Ying
Roberts, Todd
Schoggins, John W.
Xu, Wei
Anterograde Transneuronal Tracing and Genetic Control with Engineered Yellow Fever Vaccine YFV-17D
title Anterograde Transneuronal Tracing and Genetic Control with Engineered Yellow Fever Vaccine YFV-17D
title_full Anterograde Transneuronal Tracing and Genetic Control with Engineered Yellow Fever Vaccine YFV-17D
title_fullStr Anterograde Transneuronal Tracing and Genetic Control with Engineered Yellow Fever Vaccine YFV-17D
title_full_unstemmed Anterograde Transneuronal Tracing and Genetic Control with Engineered Yellow Fever Vaccine YFV-17D
title_short Anterograde Transneuronal Tracing and Genetic Control with Engineered Yellow Fever Vaccine YFV-17D
title_sort anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine yfv-17d
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665090/
https://www.ncbi.nlm.nih.gov/pubmed/34824475
http://dx.doi.org/10.1038/s41592-021-01319-9
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