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Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study

AIM: The objective of this study was to evaluate the inflammatory process resulting from the use of two cyanoacrylate-based adhesives to stabilize grafts. METHODOLOGY: A total of 45 male Wistar rats were randomly divided into three groups (n = 15/group) treated with ethyl cyanoacrylate glue (TG1), o...

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Autores principales: Paula, Dayrine Silveira de, Lima, Lia Vila Real, Cavalcante, Roberta Barroso, Silva, Paulo Goberlânio de Barros, Alves, Ana Paula Negreiros Nunes, Silva, Bruno Rocha da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665204/
https://www.ncbi.nlm.nih.gov/pubmed/34938051
http://dx.doi.org/10.1016/j.sdentj.2021.05.002
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author Paula, Dayrine Silveira de
Lima, Lia Vila Real
Cavalcante, Roberta Barroso
Silva, Paulo Goberlânio de Barros
Alves, Ana Paula Negreiros Nunes
Silva, Bruno Rocha da
author_facet Paula, Dayrine Silveira de
Lima, Lia Vila Real
Cavalcante, Roberta Barroso
Silva, Paulo Goberlânio de Barros
Alves, Ana Paula Negreiros Nunes
Silva, Bruno Rocha da
author_sort Paula, Dayrine Silveira de
collection PubMed
description AIM: The objective of this study was to evaluate the inflammatory process resulting from the use of two cyanoacrylate-based adhesives to stabilize grafts. METHODOLOGY: A total of 45 male Wistar rats were randomly divided into three groups (n = 15/group) treated with ethyl cyanoacrylate glue (TG1), octyl-2-cyanoacrylate glue (TG2) or suture threads (CG). After de-epithelialization in the anterior gingival region of the mandible, the graft was removed from the donor site (hard palate), taken to the recipient site and stabilized according to the protocol of each group. After 7, 14, and 45 days, the animals were euthanized. The graft area was analysed macroscopically, histologically, histochemically (Masson trichrome), and immunohistochemically positive cell count for TGF-β, α-SMA, RANKL, OPG, FGF, and IL-10. The Kruskal-Wallis/Dunn test (SPSS 20.0, p < 0.05) was used for analysis. RESULTS: There was no difference in the clinical parameters among the three groups, but TG1 showed the lowest mononuclear inflammatory cell count and the highest amount of total collagen. FGF immunoexpression was significantly higher for the CG group, but the TG2 showed a significant reduction in the RANKL/OPG ratio. CONCLUSION: TG1 had a mild inflammatory response and a higher collagen deposition than other glues, and TG2 had a reduction in the RANKL / OPG ratio.
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spelling pubmed-86652042021-12-21 Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study Paula, Dayrine Silveira de Lima, Lia Vila Real Cavalcante, Roberta Barroso Silva, Paulo Goberlânio de Barros Alves, Ana Paula Negreiros Nunes Silva, Bruno Rocha da Saudi Dent J Original Article AIM: The objective of this study was to evaluate the inflammatory process resulting from the use of two cyanoacrylate-based adhesives to stabilize grafts. METHODOLOGY: A total of 45 male Wistar rats were randomly divided into three groups (n = 15/group) treated with ethyl cyanoacrylate glue (TG1), octyl-2-cyanoacrylate glue (TG2) or suture threads (CG). After de-epithelialization in the anterior gingival region of the mandible, the graft was removed from the donor site (hard palate), taken to the recipient site and stabilized according to the protocol of each group. After 7, 14, and 45 days, the animals were euthanized. The graft area was analysed macroscopically, histologically, histochemically (Masson trichrome), and immunohistochemically positive cell count for TGF-β, α-SMA, RANKL, OPG, FGF, and IL-10. The Kruskal-Wallis/Dunn test (SPSS 20.0, p < 0.05) was used for analysis. RESULTS: There was no difference in the clinical parameters among the three groups, but TG1 showed the lowest mononuclear inflammatory cell count and the highest amount of total collagen. FGF immunoexpression was significantly higher for the CG group, but the TG2 showed a significant reduction in the RANKL/OPG ratio. CONCLUSION: TG1 had a mild inflammatory response and a higher collagen deposition than other glues, and TG2 had a reduction in the RANKL / OPG ratio. Elsevier 2021-12 2021-05-08 /pmc/articles/PMC8665204/ /pubmed/34938051 http://dx.doi.org/10.1016/j.sdentj.2021.05.002 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Paula, Dayrine Silveira de
Lima, Lia Vila Real
Cavalcante, Roberta Barroso
Silva, Paulo Goberlânio de Barros
Alves, Ana Paula Negreiros Nunes
Silva, Bruno Rocha da
Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study
title Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study
title_full Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study
title_fullStr Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study
title_full_unstemmed Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study
title_short Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study
title_sort fixation of free gingival grafts with cyanoacrylate glues: a histomorphometric and immunohistochemical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665204/
https://www.ncbi.nlm.nih.gov/pubmed/34938051
http://dx.doi.org/10.1016/j.sdentj.2021.05.002
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