Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system

Human induced pluripotent stem cells (iPSCs) have great promise in regenerative medicine. However, several limitations, including immune-incompatibility, have raised concerns regarding their clinical application. Recent studies have shown that human iPSCs and their derivatives lose their immunogenic...

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Autores principales: Romano, Elena, Trionfini, Piera, Giampietro, Roberta, Benigni, Ariela, Tomasoni, Susanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Lab Resource: Genetically-Modified Single Cell Line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665218/
https://www.ncbi.nlm.nih.gov/pubmed/34688128
http://dx.doi.org/10.1016/j.scr.2021.102580
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author Romano, Elena
Trionfini, Piera
Giampietro, Roberta
Benigni, Ariela
Tomasoni, Susanna
author_facet Romano, Elena
Trionfini, Piera
Giampietro, Roberta
Benigni, Ariela
Tomasoni, Susanna
author_sort Romano, Elena
collection PubMed
description Human induced pluripotent stem cells (iPSCs) have great promise in regenerative medicine. However, several limitations, including immune-incompatibility, have raised concerns regarding their clinical application. Recent studies have shown that human iPSCs and their derivatives lose their immunogenicity when major histocompatibility complex (MHC) class I and II genes are inactivated and CD47 is over-expressed. In this study, we used CRISPR-Cas9 technology to generate an isogenic iPSC line with a homozygous frameshift mutation in the MHC II transactivator (CIITA) gene. The CIITA(-/-) iPSCs exhibit typical morphology of pluripotent cells, normal karyotype, expression of pluripotency markers and differentiation capacity in the three germ layers.
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spelling pubmed-86652182021-12-15 Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system Romano, Elena Trionfini, Piera Giampietro, Roberta Benigni, Ariela Tomasoni, Susanna Stem Cell Res Lab Resource: Genetically-Modified Single Cell Line Human induced pluripotent stem cells (iPSCs) have great promise in regenerative medicine. However, several limitations, including immune-incompatibility, have raised concerns regarding their clinical application. Recent studies have shown that human iPSCs and their derivatives lose their immunogenicity when major histocompatibility complex (MHC) class I and II genes are inactivated and CD47 is over-expressed. In this study, we used CRISPR-Cas9 technology to generate an isogenic iPSC line with a homozygous frameshift mutation in the MHC II transactivator (CIITA) gene. The CIITA(-/-) iPSCs exhibit typical morphology of pluripotent cells, normal karyotype, expression of pluripotency markers and differentiation capacity in the three germ layers. Elsevier 2021-12 /pmc/articles/PMC8665218/ /pubmed/34688128 http://dx.doi.org/10.1016/j.scr.2021.102580 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Lab Resource: Genetically-Modified Single Cell Line
Romano, Elena
Trionfini, Piera
Giampietro, Roberta
Benigni, Ariela
Tomasoni, Susanna
Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
title Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
title_full Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
title_fullStr Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
title_full_unstemmed Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
title_short Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
title_sort generation of a homozygous ciita knockout ips cell line using the crispr-cas9 system
topic Lab Resource: Genetically-Modified Single Cell Line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665218/
https://www.ncbi.nlm.nih.gov/pubmed/34688128
http://dx.doi.org/10.1016/j.scr.2021.102580
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